Elsevier

Virology

Volume 240, Issue 2, 15 January 1998, Pages 175-182
Virology

Regular Article
Identification and Characterization of a Protein Kinase-Interacting Protein Encoded by theAutographa californicaNuclear Polyhedrosis Virus

https://doi.org/10.1006/viro.1997.8944Get rights and content
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Abstract

We have used a yeast two-hybrid system to identify a baculoviral gene encoding a protein kinase-interacting protein (PKIP). Thepkipgene is located at 15.8–16.2 m.u (map units) and represents ORF 24 in the sequence of978944of theAutographa californicanuclear polyhedrosis virus. PKIP is a 19.2-kDa protein and appears to stimulate the activity of the viral protein kinase-1in vitro.Northern blotting analysis revealed thatpkipis a late gene. Attempts to plaque purify apkipvirus were unsuccessful.

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1

Present address: Department of Pharmacology, University of Pennsylvania, School of Medicine, Philadelphia, PA 19104-6084.

2

To whom reprint requests should be addressed.