Elsevier

Virology

Volume 239, Issue 1, 8 December 1997, Pages 186-197
Virology

Regular Article
Two Domains of the AL1 Protein Mediate Geminivirus Origin Recognition

https://doi.org/10.1006/viro.1997.8869Get rights and content
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Abstract

The geminiviruses tomato golden mosaic virus (TGMV) and bean golden mosaic virus (BGMV) have bipartite genomes. Their A and B DNA components containcis-acting sequences that function as origins of replication, while their A components encode thetrans-acting replication proteins—AL1 and AL3. Earlier experiments demonstrated that virus-specific interactions between thecis- andtrans-acting functions are required for TGMV and BGMV replication and that the AL1 proteins of the two viruses specifically bind their respective origins. In the current study, characterization of AL1 and AL3 proteins produced from plant expression cassettes in transient replication assays revealed that interaction between AL1 and the origin is responsible for virus-specific replication. The AL3 protein does not contribute to specificity but can be preferred by its cognate AL1 protein when replication is impaired. Analysis of chimeric proteins showed that two regions of AL1 act as specificity determinants during replication. The first domain is located between amino acids 1 and 116 and recognizes the AL1 origin binding site. The second region, which is between amino acids 121 and 209, is not dependent on the known AL1 DNA binding site. Analysis of wild type and chimeric proteins in transient transcription assays showed that AL1 also represses its own promoter in a virus-specific manner. Transcriptional specificity is conferred primarily by AL1 amino acids 1–93 with amino acids 121–209 making a smaller contribution. Together, these results demonstrated that the virus-specific interactions of AL1 during replication and transcription are complex, involving at least two discreet domains of the protein.

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