Regular ArticleSingle Amino Acid Changes in the S2 Subunit of the MHV Surface Glycoprotein Confer Resistance to Neutralization by S1 Subunit-Specific Monoclonal Antibody
References (0)
Cited by (33)
Coronavirus pathogenesis
2011, Advances in Virus ResearchCitation Excerpt :Further evidence for cooperation among spike domains, noncontiguous in the primary structure comes from the observation that an E1035D substitution within HR1 of S2 may overcome the Q159L substitution in the RBD, since a spike with both of these substitutions confers hepatotropism upon a recombinant A59 (Navas-Martin et al., 2005). Escape mutants selected by resistance to a monoclonal antibody mapping to the RBD had point mutations in the region of HR2 (Grosse and Siddell, 1994), providing further support for the interaction of these domains. Thus, the high neurovirulence conferred by the JHM.SD spike can be thought of as a perfect storm.
Role of spike protein endodomains in regulating coronavirus entry
2009, Journal of Biological ChemistryCitation Excerpt :SDS gels were transferred to polyvinylidene difluoride membranes that were subsequently blocked for 1 h with 5% nonfat milk powder in TBS-T (25 mm Tris-HCl (pH 7.5), 140 mm NaCl, 2.7 mm KCl, 0.05% Tween 20). S proteins were detected with murine mAb 10G (45) (1:2000 in TBS-T). M proteins were detected with murine mAb J.3.1 (46) (1:500 in TBS-T).