Regular ArticleA Unique Conformation at the Carboxyl Terminus of the Small Hepatitis Delta Antigen Revealed by a Specific Monoclonal Antibody
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A screening assay for the identification of host cell requirements and antiviral targets for hepatitis D virus infection
2017, Antiviral ResearchCitation Excerpt :Before, we had been using a polyclonal anti-HDAg antibody (Pereira et al., 2015) like most other HDV studies (Lin et al., 1999; Wu et al., 1992). Some studies reported the application of murine monoclonal anti-HDAg antibodies that they generated in their laboratories (Hwang and Lai, 1993; Yan et al., 2012), but these have not been made commercially available to our knowledge. A monoclonal antibody offers the advantages of low background and – in hybridoma form – unlimited supply for future studies.
Histone H1e interacts with small hepatitis delta antigen and affects hepatitis delta virus replication
2008, VirologyCitation Excerpt :Protein samples were separated by SDS-polyacrylamide gel electrophoresis (10% polyacrylamide) and electrotransferred to nitrocellulose membranes. The membranes were first incubated with 5% nonfat milk and then with a human anti-HDAg antibody or an N-terminus specific monoclonal mouse anti-HDAg antibody, a byproduct from the study of Hwang and Lai (1993). The horseradish peroxidase-conjugated rabbit anti-human (Promega) or goat anti-mouse (Promega) immunoglobulin antibody was used as the second antibody.
Characterization of a strain-specific monoclonal antibody to hepatitis delta virus antigen
2000, Journal of Virological MethodsCell cycle arrest mediated by hepatitis delta antigen
1999, FEBS Letters