Regular ArticleExpression and Purification of a Spider Silk Protein: A New Strategy for Producing Repetitive Proteins
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Novel insights into construct toxicity, strain optimization, and primary sequence design for producing recombinant silk fibroin and elastin-like peptide in E. coli
2023, Metabolic Engineering CommunicationsSynthetic biology-guided design and biosynthesis of protein polymers for delivery
2023, Advanced Drug Delivery ReviewsSecretory production of spider silk proteins in metabolically engineered Corynebacterium glutamicum for spinning into tough fibers
2022, Metabolic EngineeringCitation Excerpt :Many attempts have been made to produce recombinant spidroins in a variety of heterologous hosts (Chung et al., 2012; Whittall et al., 2021). The traditional approach is to accumulate recombinant silk proteins in the cytosol of bacterial or insect cells (Foong et al., 2020; Huemmerich et al., 2004; Lewis et al., 1996; Schmuck et al., 2021) or in the endoplasmic reticulum of plant leaves (Scheller et al., 2001). A major limitation of this approach is the confined cell volume leading to the difficulty in maintaining solubility of the overexpressed silk proteins, the restriction on protein yield, and extensive cell lysis and purification steps to harvest the target proteins (Huemmerich et al., 2004; Winkler et al., 2000; Wei et al., 2020; Zhang et al., 2008).
Large scale production of synthetic spider silk proteins in Escherichia coli
2021, Protein Expression and PurificationCitation Excerpt :As spiders cannot be farmed like silkworms because of their territorial and aggressive nature [5,6], the production of recombinant spider silks is the only feasible solution for large-scale production. With new advances in genetic engineering techniques, various heterologous hosts, including microbial systems, have been explored to produce recombinant spider silks [7], but large-scale production is still challenging as producing enough spider silk is the biggest hurdle to commercial use [8,9]. Orb-weaving spiders produce eight different silk proteins.
Host Systems for the Production of Recombinant Spider Silk
2021, Trends in Biotechnology
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To whom correspondence should be addressed at the Department of Molecular Biology, Box 3944, University of Wyoming, Laramie, WY 82071-3944. Fax: (307) 766-5098; E-mail: [email protected].
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Current address: Cardiovascular Research Institute, Box 0130, University of California, San Francisco, CA 94143-0130.