Regular Article
POSSIBLE PROTECTIVE EFFECT OF MELATONIN AND/OR DESFERRIOXAMINE AGAINST STREPTOZOTOCIN-INDUCED HYPERGLYCAEMIA IN MICE

https://doi.org/10.1006/phrs.1999.0614Get rights and content

Abstract

There is a clear link between diabetes and oxidative stress. Hyperglycaemia leads to free radical generation and alteration of endogenous antioxidants. The present study is an attempt to evaluate the possible protective effect of melatonin (MLT) and/or desferrioxamine (DF) against streptozotocin (STZ)-induced hyperglycaemia in mice. Serum lipid profile, pancreatic tissue contents of glutathione (GSH) and malondialdehyde (MDA) were determined. MLT and/or DF were given p.o. in doses of 5 mg kg−1day−1and 250 mg kg−1day−1, respectively for 15 consecutive days prior to STZ treatment (60 mg kg−1day−1i.p.) for 3 consecutive days. Results revealed that STZ induced a marked increase in serum glucose, serum triglycerides (TG), cholesterol (CHO) andLDL-cholesterol . On the contrary HDL-cholesterol was markedly decreased in STZ-treated group. Moreover, STZ induced a significant decrease in the pancreatic content of GSH with concomitant increase in MDA content. Administration of MLT or (MLT+DF) prior to STZ treatment revealed a marked decrease in serum glucose level by 35.6 and 31.6%, respectively as compared to STZ-treated group. Furthermore, MLT pretreatment of STZ-induced hyperglycemic mice, has not only normalized GSH content of pancreatic tissues but also increased its level more than that of control animals by 110%. On the contrary, MDA content of pancreatic tissues was markedly decreased even lower than normal control group. MLT also, induced a marked protection in terms of decreasing serum CHO, LDL, TG by 21.8, 83.8 and 82.2%, respectively, while HDL was increase by 56% as compared to STZ treated group. DF was found to be less effective than MLT in the protection against STZ-induced hyperglycemia. In conclusion, these data suggest that MLT protects against the damaging consequences induced by hyperglycemia either systemically or in the pancreatic tissues.

References (26)

  • YH Abdel-Wahab et al.

    Glycation of insulin in the islets of Langerhans of normal and diabetic animals

    Diabetes

    (1996)
  • D Giugliano et al.

    Oxidative stress and diabetic vascular complications

    Diabetes Care

    (1996)
  • Cited by (55)

    • Combined treatment with melatonin and insulin improves glycemic control, white adipose tissue metabolism and reproductive axis of diabetic male rats

      2018, Life Sciences
      Citation Excerpt :

      Some studies have demonstrated that melatonin reduces hyperglycemia and hyperlipidemia in STZ-induced diabetic animals and the hiperinsulinemia in Otsuka Long-Evans Tokushima Fatty (OLEFT) animals [12]. Additionally, melatonin treatment of diabetic animals reduced glycemia in comparison to non-treated animals [13]. On the other hand, another study demonstrated that diabetic animals treated with melatonin via intraperitoneal injections did not present reduced glycemic levels [14].

    • Effect of Melatonin as an Antioxidant in the Liver

      2018, The Liver: Oxidative Stress and Dietary Antioxidants
    • Attenuation of acute and chronic liver injury by melatonin in rats

      2013, Journal of Taibah University for Science
      Citation Excerpt :

      Antioxidants can help to minimize the oxidative stress, in cirrhotic livers [24]. Melatonin (N-acetyl-5-metyoxytryptamine) is a powerful endogenous antioxidant, plays very important roles in regulation of circadian rhythms, sleep, immune system activity, and elimination of oxygen free radicals [25,26], and exerts its antioxidant effects mainly through G protein dependent receptors which lead to the induction of antioxidant enzyme synthesis [27]. In this way, melatonin neutralizes the effects of both oxygen- and nitrogen-based reactive molecules [28].

    View all citing articles on Scopus

    Unspecified

    View full text