Quantitative Study of Changes in Intestinal Morphology and Mucus Gel on Total Parenteral Nutrition in Rats

doi:10.1006/jsre.2000.5937

Journal of Surgical Research

Volume 94, Issue 2, December 2000, Pages 99-106

https://doi.org/10.1006/jsre.2000.5937 Get rights and content

Abstract

Quantification of changes in gastrointestinal morphology and mucus gel has been difficult to study. In the present study, we investigated changes in rat intestine under total parenteral nutrition (TPN) using fluoresceinated lectin staining and image analysis. Wistar rats (n = 34) were divided into two groups: one group received TPN for 2 weeks, and a control group received standard rat chow and water ad libitum for the same period. A 1-cm segment of distal ileum was removed and cut into cross sections. Sections were stained with hematoxylin and eosin, and to stain the mucus, periodic acid–Schiff (PAS), alcian blue (AB), and fluoresceinated lectin, that is, FITC-labeled Ulex europaeus agglutinin I (FITC-UEA-I), were used. Light microscope images were stored in a personal computer and analyzed using image analysis. We measured perimeter length, mucosal thickness, villus area, villus surface area index, mucus stain-positive area, mucosal area ratio, and mucosal surface area ratio. Perimeter length, mucosal thickness, villus area, and villus surface area index in the TPN group were significantly less than those in the control group (P < 0.001 for each parameter). In all mucus stainings, the stain-positive area in the TPN group was significantly less than that in the control group. However, there were no significant differences in mucosal area or mucosal surface area ratios between the two groups. The FITC-UEA-I-positive area was significantly greater than the PAS- or and AB-positive area. There were significant positive correlations between the FITC-UEA-I-positive area and both the PAS-positive and AB-positive areas. TPN for 2 weeks promoted intestinal atrophy and decreased absolute quantity of mucus gel. We successfully introduced the FITC-UEA-I staining method to evaluate changes in mucus gel.

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Regular ArticleQuantitative Study of Changes in Intestinal Morphology and Mucus Gel on Total Parenteral Nutrition in Rats

Abstract

Jpn. J. Pharmacol.

Adv. Carbohydr. Chem. Biochem.

Biochim. Biophys. Acta

Exp. Cell Res.

Total parenteral nutrition promotes bacterial translocation from the gut

Surgery

Fiber: Effect on bacterial translocation and intestinal mucin content

World J. Surf.

The search for appropriate measurements of proliferative and morphological status in studies on intestinal adaptation

Role of bacterial adherence and the mucus barrier on bacterial translocation: Effect of protein malnutrition and endotoxin in rats

Ann. Surg.

Studies on the localization and the measurement of macromolecular glycoproteins of the glandular stomach in rats

Jpn. J. Gastroenterol.

Study of mucous barrier as a defensive factor of gastric mucosa

Jpn. J. Gastroenterol.

Pathogenesis of peptic ulcer and changes of protective mechanism of the gastric and duodenal mucosa

Jpn. J. Gastroenterol.

The study of the image analysis of the gastric mucus: Including phospholipid as the mucosal barrier

Stomach Intestine

Mucosal architecture and epithelial cell production rate in the small intestine of the albino rat

J. Anat.

The life history of cells in renewing systems

Am. J. Anat.

Effects of fasting on villi along the small intestine: A stereological approach to the problem of quantifying villus ‘shape’

Experientia

Effects of fasting on mucosal dimensions in the duodenum, jejunum and ileum of the rat

J. Anat.

The effect of starvation and refeeding on cell population kinetics in the rat small bowel mucosa

J. Anat.

The number of villi in rat's jejunum and ileum: Effect of normal growth, partial enterectomy, and tube feeding

J. Anat.

The effect of growth and fasting on the number of villi and crypts in the small intestine of albino rat

J. Anat.

Mechanisms of adaptation in rat small intestine: Regional differences in quantitative morphology during normal growth and experimental hypertrophy

J. Anat.

Regular Article
Quantitative Study of Changes in Intestinal Morphology and Mucus Gel on Total Parenteral Nutrition in Rats