Journal of Molecular Biology
Characterization of a Non-long Terminal Repeat Retrotransposon cDNA (L1Tc) fromTrypanosoma cruzi: Homology of the First ORF with the Ape Family of DNA Repair Enzymes
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Variability of the Pr77 sequence of L1Tc retrotransposon among six T. cruzi strains belonging to different discrete typing units (DTUs)
2021, Acta TropicaCitation Excerpt :The sequencing of trypanosomatid genomes has revealed that they all contain a large number of retrotransposons (active or degenerate) that together make up to 5% of the nuclear genome (El-Sayed et al., 2005b). Among trypanosomatids, the best characterized LINE is L1Tc of T. cruzi (Martin et al., 1995; Thomas et al., 2010) which, together with the ingi element of T. brucei (Tbingi) conforms the ingi/L1Tc clade (Bringaud et al., 2006). L1Tc encodes its own retrotransposition machinery: AP endonuclease (Olivares et al., 1997), reverse transcriptase (García-Pérez et al., 2003), RNase H (Olivares et al., 2002) and nucleic acid chaperone protein (Heras et al., 2005).
Retrotransposons in pluripotent cells: Impact and new roles in cellular plasticity
2015, Biochimica et Biophysica Acta - Gene Regulatory MechanismsCitation Excerpt :ORF1p codes for an RNA binding protein with nucleic acid chaperone activity [20–23]. ORF2p encodes a protein with at least two demonstrated enzymatic activities, an Apurinic/Apyrimidinic (AP)-like endonuclease (EN) [24,25] and a reverse transcriptase (RT) [26]. RC-L1s also contain a short 3′UTR, and ends in a poly(A) tail [12,13].
TSIDER1, a short and non-autonomous Salivarian trypanosome-specific retroposon related to the ingi6 subclade
2011, Molecular and Biochemical ParasitologyThe biology and evolution of transposable elements in parasites
2010, Trends in ParasitologyCitation Excerpt :This ORF codes for at least a protein having RT and endonuclease activities (EN) [15]. A list of non-LTR retrotransposons from parasites of mammals and some of their characteristics are presented in Table 1[16,19,25,27,36–48]. Given the crucial functional role that the LTRs play in the mobilization of LTR-retrotransposons, it is likely that non-LTR retrotransposons use a different retrotransposition–integration mechanism.
Mechanisms of DNA repair in Trypanosoma cruzi: What do we know so far?
2020, DNA RepairCitation Excerpt :Studies using a negative dominant form of TcAP1 (TcAP1DN) [11] or human APE1 (hAPE1DN) confirmed that BER and AP endonuclease activity plays relevant roles in the resistance of oxidative damage in these parasites and contributes to parasite survival inside the mammalian and insect host organisms. Interestingly, Martin et al. discovered a fragment of T. cruzi’s retrotransposon with homology with APE-1 [38]. This protein, called NL1Tc, successfully complemented the DNA repair deficiency in mutant bacteria [39] and conferred superior DNA repair capacity to overexpressing T. cruzi clones [40].
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