Regular Article
Postharvest Control of Litchi Fruit Rot by Bacillus subtilis

https://doi.org/10.1006/fstl.2001.0758Get rights and content

Abstract

The litchi (Litchi chinensis Sonn.) fruit are extremely susceptible to postharvest moulds. The main pathogen of litchi fruit was isolated and identified as Peronophythora litchi. Of antagonists tested, Bacillus subtilis was the most effective against Peronophythora litchi. The relative antifungal activity of Bacillus subtilis reached a maximum after 48 h culture, and then decreased gradually. Purification of the crude extract of Bacillus subtilis followed by a Sepharose Toyopearl HW-50 gel filtration confirmed that the antifungal compound of Bacillus subtilis had a molecular weight of about 1.2 kD. Both the antagonist and its extract were effective in controlling artificially wound-inoculated fruit pathogens, but the use of the extract had better effect than that of the antagonist. Furthermore, application of the extract of Bacillus subtilis exhibited good control of decay for a storage period of 30 days at 5 °C and quality maintenance of litchi fruit in terms of eating scores and contents of total soluble solids, titratable acidity and ascorbic acid. No off-flavor of the treated fruit was tasted.

References (49)

  • G. ARRAS et al.

    Biocontrol by yeasts of blue mould of citrus fruits and the mode of action of an isolate of Pichia guilliermondii

    Journal of Horticultural Science and Biotechnology

    (1998)
  • R. BARKAII-GOLAN et al.

    Postharvest heat treatments of fresh fruits and vegetables for decay control

    Plant Disease

    (1991)
  • F. BESSON et al.

    Characterization of iturin A in antibiotics from various strains of Bacillus subtilis

    Journal of Antibiotics

    (1976)
  • T. BOTHA et al.

    Swamme geassosieer met na-oesbederf by lietsjies en die beheer van na-oessiektes en verbruining met swamdoders en poli-etileenverpakking

    South African Litchi Growers' Association Yearbook 1988

    (1988)
  • T.Y. CHUANG et al.

    Biological control of mango anthracnose

    Plant Protection Bulletin (Taipei)

    (1997)
  • L.M. OATES et al.

    Postharvest disease control in lychees using sulfur dioxide treatment

    (1993)
  • S. DROBY et al.

    Antagonistic microorganisms as biological control agents of postharvest diseases of fruits and vegetables

    Post-Harvest News Information

    (1991)
  • R.C. GUELDNER et al.

    Isolation and identification of iturines as antifungal peptides in biological control of peach-brown rot withBacillus subtilis

    Journal of Agricultural and Food Chemistry

    (1988)
  • Y HUANG et al.

    Postharvest biological control of Penicillium digitatum decay on citrus fruit by Bacillus pumilus

    Annual Applied Biology

    (1992)
  • P.Y. HUANG et al.

    Control of rotting and browning of litchi fruit after harvest at ambient temperatures in China

    Tropical Agriculture

    (1990)
  • W.J. JANISIEWICZ

    Biocontrol of postharvest diseases on apples with antagonist mixture

    Phytopathology

    (1988)
  • W.J. JANISIEWICZ et al.

    Biological control of blue-mold and gray-mold on apple and pear with Pseudomonas cepacia

    Phytopathology

    (1988)
  • P. JEFFRIES et al.

    The biological control of postharvest diseases of fruit

    Postharvest News Information

    (1990)
  • Y.M. JIANG

    The use of microbial metabolites against post-harvest diseases of longan fruit

    International Journal of Food Science and Technology

    (1977)
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