Regular ArticleStructure and Function Analysis of the Calcium-Related Gene spray in Neurospora crassa
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Phenotypic abnormalities of fr, sp, and och-1 single mutants are suppressed by loss of putative GPI-phospholipase A2 in Neurospora crassa
2017, MycoscienceCitation Excerpt :This GPI anchor remodeling process is thought to be important for efficient endoplasmic reticulum-to-Golgi transport of GPI proteins. The sp is a filamentous fungus-specific gene found in N. crassa that encodes the putative calcium ion channel protein SPRAY and has seven transmembrane domains, localizes in an organelle membrane, and may contribute to the distribution of calcium ions via calcineurin (Bok et al. 2001). In S. cerevisiae, Flc, a SPRAY-like protein, is located on the endoplasmic reticulum and is involved in hypotonic stress-induced calcium signaling and cell wall maintenance (Rigamonti et al. 2015).
Hypotonic stress-induced calcium signaling in Saccharomyces cerevisiae involves TRP-like transporters on the endoplasmic reticulum membrane
2015, Cell CalciumCitation Excerpt :This gene family, has representatives in several fungal species, formerly identified as members of a calcium-related spray proteins family. The paradigm of this family is the N. crassa spray protein [71], an ER-localized calcium channel regulating thigmotropism, i.e. the ability of this organism to respond to a topographical stimulus by altering the axis of growth of the hyphae [72]. Sequence alignment reveals that fungal spray proteins are more similar to the TRPML (for mucolipin) branch of TRP channels, actually the channels most closely related to TRPP class of calcium transporters.
Regulation of vectorial supply of vesicles to the hyphal tip determines thigmotropism in Neurospora crassa
2014, Fungal BiologyCitation Excerpt :The reduction in the thigmotropic response of the spray deletion strain (Fig 2) therefore supports the hypothesis that N. crassa thigmotropism is also calcium-regulated. The spray deletion mutant has also been shown to be sensitive to calcineurin inhibitors (Bok et al. 2001). It is hypothesized that the stretch-activated PLC and its role in calcium homoeostasis at the hyphal tip may therefore influence the thigmotropic response of N. crassa through a calcineurin-related mechanism.
Key differences between lateral and apical branching in hyphae of Neurospora crassa
2004, Fungal Genetics and BiologyA mutation in the Neurospora crassa actin gene results in multiple defects in tip growth and branching
2004, Fungal Genetics and Biology