Cyclin D2 ArrestsXenopusEarly Embryonic Cell Cycles

doi:10.1006/excr.1997.3800

Experimental Cell Research

Volume 237, Issue 2, 15 December 1997, Pages 338-346

https://doi.org/10.1006/excr.1997.3800 Get rights and content

Abstract

Xenopuscyclin D2 mRNA is a member of the class of maternal RNAs. It is rare and stable during early embryonic development. To investigate the potential role of cyclin D2 during early embryonic cell cycles, cyclin D2 was injected into one blastomere of a two-cell embryo. This injection induced a cell cycle arrest in the injected blastomere. To analyze more precisely the mechanism of this arrest, we took advantage of cycling egg extracts that recapitulate major events of the cell cycle when supplemented with demembranated sperm heads. WhenXenopuscyclin D2 is added to egg extracts, the first round of DNA replication occurs as in control extracts. However,Xenopuscyclin D2 blocks subsequent rounds of DNA replication and the oscillations of histone H1 kinase activity associated with cdc2 kinase, indicating that the cell cycle is arrested after the first S-phase. The block induced byXenopuscyclin D2 is not due to a lack of the mitotic cyclin B2 that accumulates normally. RadiolabeledXenopuscyclin D2 enters nuclei after completion of the first S-phase and remains stable over the entire period of the arrest. These features suggest thatXenopuscyclin D2 could play an original role during early development, controlling the G2-phase and/or the G2/M transition.

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¹: Present address: Imperial Cancer Research Fund, Clare Hall Laboratories, Blanche Lane, South Mimms, Potters Bar, Herts EN6 3LD, England.

²: To whom correspondence and reprint requests should be addressed. Fax: (33) 1 44 27 34 72. E-mail: [email protected].

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Regular ArticleCyclin D2 ArrestsXenopusEarly Embryonic Cell Cycles

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Regular Article
Cyclin D2 ArrestsXenopusEarly Embryonic Cell Cycles