Baculoviral Display of the Green Fluorescent Protein and Rubella Virus Envelope Proteins

doi:10.1006/bbrc.1997.7372

Biochemical and Biophysical Research Communications

Volume 238, Issue 3, 29 September 1997, Pages 717-722

https://doi.org/10.1006/bbrc.1997.7372 Get rights and content

Abstract

The ability to display heterologous proteins and peptides on the surface of different types of bacteriophage has proven extremely useful in protein structure/function studies. To display such proteins in a eucaryotic environment, we have produced a vector allowing for fusion of proteins to the amino-terminus of theAutographa californicanuclear polyhedrosis virus (AcNPV) major envelope glycoprotein, gp64. Such fusion proteins incorporate into the baculoviral virion and display the FLAG epitope tag. We have further produced recombinant baculoviruses displaying the green fluorescent protein (GFP) and the rubella virus envelope proteins, E1 and E2. The incorporation of the GFPgp64, E1gp64, and E2gp64 fusion proteins into the baculovirus particle was demonstrated by western blot analysis of purified budded virus. This is the first report of the display of the GFP protein or the individual rubella virus spike proteins on the surface of an enveloped virus. Such a eucaryotic viral display system may be useful for the display of proteins dependent on glycosylation for activity and for targeting of recombinant baculoviruses to novel host cell types as a gene transfer vehicle.

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Regular ArticleBaculoviral Display of the Green Fluorescent Protein and Rubella Virus Envelope Proteins

Abstract

Trends Biotechnol.

Curr. Opin. Struct. Biol.

J. Mol. Biol.

Virology

Virology

FEBS Lett.

Adv. Virus Res.

Virology

Virology

Curr. Opin. Struct. Biol.

Gene

Annu. Rev. Immunol.

Baculovirus Expression Vectors, a Laboratory Manual

J. Virol.

J. Virol.

Eur. J. Biochem.

J. Virol.

J. Virol.

Bio/Technology

Science

Regular Article
Baculoviral Display of the Green Fluorescent Protein and Rubella Virus Envelope Proteins