Accumulation of β-1,3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems toPhytophthora capsici

doi:10.1006/pmpp.1997.0119

Physiological and Molecular Plant Pathology

Volume 51, Issue 5, November 1997, Pages 305-322

https://doi.org/10.1006/pmpp.1997.0119 Get rights and content

Abstract

Pepper (Capsicum annuumL.) plants sprayed with DL-β-amino-n-butyric acid (BABA) were protected againstPhytophthora capsiciinfection. BABA treatment induced the synthesis and accumulation of β-1,3-glucanases and chitinases in the stem tissues of pepper plants. Their accumulation was very pronounced in the stems challenge-inoculated withP. capsiciafter BABA treatment. Several β-1,3-glucanase and chitinase isoforms accumulated in BABA treatedP. capsici. When analysed by immunoblot of the denatured proteins, the 20 kDa β-1,3-glucanase and 32 kDa chitinase were found in pepper stems treated with BABA and/or infected byP. capsici. BABA treatment did not stimulate capsidiol production in pepper stems, but prior treatment led to high accumulation inP. capsici-infected ones. Unlike capsidiol production, BABA treatment triggered a dramatic increase in the endogenous levels of salicylic acid (SA) in pepper stems. The increase in endogenous SA was much pronounced inP. capsiciinfected stems after BABA treatment. In conclusion, the induction of resistance toP. capsiciin pepper plants by BABA treatment positively correlated with the accumulation of certain β-1,3-glucanase and chitinase isoforms, and SA. These results suggest strongly that SA may act as an endogenous signal responsible for activating particular components of resistance toP. capsiciand the induction of pathogenesis-related proteins such as β-1,3-glucanase and chitinase.

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Regular articleAccumulation of β-1,3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems toPhytophthora capsici☆

Abstract

Analytical Biochemistry

Physiological and Molecular Plant Pathology

Analytical Biochemistry

Physiological and Molecular Plant Pathology

Physiological and Molecular Plant Pathology

Plant Science

Physiological and Molecular Plant Pathology

Analytical Biochemistry

Virology

Resistance to disease in the hybridNicotiana glutinosa×Nicotiana debneyi

Physiological and Molecular Plant Pathology

Light-induced extracellular accumulation of b (pathogenesis-related) proteins inNicotiana

Canadian Journal of Botany

Induction of hydrolases as a defense reaction against pathogens

Cellular and Molecular Biology of Plant Stress

Hydrolytic enzymes in plant disease resistance

Chitinase in bean leaves: induction by ethylene, purification, properties, and possible function

Planta

β-Aminobutyric acid induces the accumulation of pathogenesis-related proteins in tomato (Lycopersicon esculentumPhytophthora infestans.

Plant Physiology

Disc electrophoresis-II method and application to human serum proteins

Annals of the New York Academy of Science

Requirement of salicylic acid for the induction of systemic acquired resistance

Science

Effects of age-related resistance and metalaxyl for capsidiol production in pepper plants infected withPhytophthora capsici

Capsidiol production in pepper plants associated with age-related resistance toPhytophthora capsici.

Korean Journal of Plant Pathology

Effect of metalaxyl on capsidiol production in stems of pepper plants infected withPhytophthora capsici.

Plant Disease

Soluble proteins, esterases and superoxide dismutase in stem tissue of pepper plants in relation to age-related resistance toPhytophthora capsici.

Journal of Phytopathology

Identification of several pathogenesis-related proteins in tomato leaves inoculated withCladosporium fulvumFulvia fulva

Plant Physiology

Biological function of pathogenesis-related proteins: four PR proteins of tobacco have β-1,3-glucanase activity

The EMBO Journal

β-1,3-Endoglucanases from soybean releases elicitor-active carbohydrates from fungus cell walls

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Regular article
Accumulation of β-1,3-glucanase and chitinase isoforms, and salicylic acid in the DL-β-amino-n-butyric acid-induced resistance response of pepper stems toPhytophthora capsici☆