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Linkage of a RAPD marker with powdery mildew resistance er-1 gene in Pisum sativum L.

  • Plant Genetics
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Abstract

The aim of this study was to investigate the inheritance of powdery mildew disease and to tag it with a DNA marker to utilize for the marker-assisted selection (MAS) breeding program. The powdery mildew resistant genotype Falloner and susceptible genotype 11760-3ER were selected from 177 genotypes by heavy infestation of germplasm with Erysiphe pisi through artificial inoculation The F1 plants of the cross Fallon/11760-3 indicated the dominance of the susceptible allele, while F2 plants segregated in 3: 1 ratio (susceptible: resistant) that fit for goodness of fitness by χ2 (P > 0.07), indicating monogenic recessive inheritance for powdery mildew resistance in Pisum sativum. A novel RAPD marker OPB18 (5′-CCACAGCAGT-3′) was linked to the er-1 gene with 83% probability with a LOD score of 4.13, and was located at a distance of 11.2 cM from the er-1 gene.

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Authors and Affiliations

  1. Department of Botany, Nuasheen University of Malakand, Khyber Pakhtunkhwa, Pakistan

    M. Nisar

  2. Plant Genetic Resources Program, National Agricultural Research Centre, Islamabad, Pakistan

    A. Ghafoor

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  1. M. Nisar
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  2. A. Ghafoor
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Correspondence to M. Nisar.

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Nisar, M., Ghafoor, A. Linkage of a RAPD marker with powdery mildew resistance er-1 gene in Pisum sativum L.. Russ J Genet 47, 300–304 (2011). https://doi.org/10.1134/S102279541102013X

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  • DOI: https://doi.org/10.1134/S102279541102013X

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