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New occurrence of microchromosomes B in Moenkhausia sanctaefilomenae (Pisces, Characidae) from the Paraná River of Brazil: analysis of the synaptonemal complex

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Abstract

The Moenkhausia sanctaefilomenae specimens showed a karyotype consisting of 2n = 50 chromosomes with 12 metacentrics, 36 submetacentrics and two subtelocentrics. In addition to the basic karyotype, all the males specimens have cells ranging from zero to two B microchromosomes in mitotic metaphases. These chromosomes were not observed in the female specimens. C-band analysis showed a distribution pattern of characteristic heterochromatin with interstitial and centromeric blocks. However, the B chromosomes were faintly stained with C-banding and were not fluorescent with CMA3 staining. The meiotic studies showed the formation of bivalents in metaphase I and in pachytene under an optical microscope. Through synaptonemal complex analysis with an electron microscope, the pachytene showed 25 bivalents completely paired and a small bivalent corresponding to the B chromosomes. In the same preparation, one of the B chromosomes was observed in a univalent form. On the basis of pairing behavior and morphology it is assumed that B chromosomes of M. sanctaefilomenae show homology between them and their evolutionary aspects are discussed.

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Authors and Affiliations

  1. Departamento de Biologia Celular e Genética, Nupelia, Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais, Universidade Estadual de Maringá, CEP: 87020-900, Maringá, Paraná, Brasil

    Ana Luiza de Brito Portela-Castro

  2. Departamento de Biologia Celular e Genética, Nupelia, Pós-Graduação em Ecologia de Ambientes Aquáticos Continentais, Universidade Estadual de Maringá, CEP: 87020-900, Maringá, Paraná, Brasil

    Horácio Ferreira Júlio Júnior

  3. Departamento de Morfologia/UNESP, Botucatu, CEP: 18618-000, Botucatu, SP, Brasil

    Patrícia Belini Nishiyama

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  1. Ana Luiza de Brito Portela-Castro
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  2. Horácio Ferreira Júlio Júnior
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  3. Patrícia Belini Nishiyama
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Portela-Castro, A.L.d., Júnior, H.F.J. & Nishiyama, P.B. New occurrence of microchromosomes B in Moenkhausia sanctaefilomenae (Pisces, Characidae) from the Paraná River of Brazil: analysis of the synaptonemal complex. Genetica 110, 277–283 (2000). https://doi.org/10.1023/A:1012742717240

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