Biochemical and Biophysical Research Communications
Direct reciprocal effects of resistin and adiponectin on vascular endothelial cells: a new insight into adipocytokine–endothelial cell interactions
Section snippets
Materials and methods
Materials. Human recombinant resistin and mouse recombinant globular domain of adiponectin/Acrp30 were purchased from WAKO (Japan). Pitavastatin was kindly provided by Kowa (Japan).
Cell culture. Human aortic endothelial cells (HAECs, purchased from Bio-Whittaker) were cultured in endothelial cell growth medium-2 (EGM-2, Bio-Whittaker) at 37 °C in a humidified atmosphere of 5% CO2. Bovine aortic endothelial cells (BAECs, purchased from Bio-Whittaker) were cultured in Dulbecco’s modified Eagle’s
Resistin induces expression of adhesion molecules and pentraxin 3 in HAECs
To determine whether resistin directly affects vascular endothelial cell functions, we stimulated HAECs with human recombinant resistin and examined gene expression of adhesion molecules and of the inflammatory marker pentraxin 3 (PTX3), which shares high homology with C-reactive protein (CRP). Northern blot analysis revealed that resistin (30 nM) increased VCAM-1, ICAM-1, and PTX3 mRNA expression by 2 h, reaching a maximum at 4 or 8 h after resistin stimulation in HAECs (Fig. 1A). Resistin
Discussion
In this study, we have demonstrated that resistin induced mRNA expression of adhesion molecules and PTX3 in HAECs. Induction of VCAM-1 and ICAM-1 was confirmed also at protein level. Interestingly, pitavastatin partially prevented VCAM-1 induction by resistin. More importantly, adiponectin dramatically inhibited VCAM-1 induction by resistin in HAECs.
While we were preparing this manuscript, Verma et al. [10] also reported that resistin upregulated VCAM-1, endothelin-1, and monocyte
Acknowledgements
We thank Chie Fujinami for technical assistance. This study was supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science and Culture, Japan.
References (22)
- et al.
Disruption of adiponectin causes insulin resistance and neointimal formation
J. Biol. Chem.
(2002) - et al.
Globular adiponectin protected ob/ob mice from diabetes and ApoE-deficient mice from atherosclerosis
J. Biol. Chem.
(2003) - et al.
Generation of a dominant-negative mutant of endothelial PAS domain protein 1 by deletion of a potent C-terminal transactivation domain
J. Biol. Chem.
(1999) - et al.
Resistin is expressed in human macrophages and directly regulated by PPAR gamma activators
Biochem. Biophys. Res. Commun.
(2003) - et al.
Interleukin-1-inducible genes in endothelial cells. Cloning of a new gene related to C-reactive protein and serum amyloid P component
J. Biol. Chem.
(1992) Beyond lipid lowering: the role of statins in vascular protection
Int. J. Cardiol.
(2002)- et al.
Fibrate and statin synergistically increase the transcriptional activities of PPARalpha/RXRalpha and decrease the transactivation of NFkappaB
Biochem. Biophys. Res. Commun.
(2002) Adiponectin and resistin—new hormones of white adipose tissue
Med. Sci. Monit.
(2003)- et al.
Diet-induced insulin resistance in mice lacking adiponectin/ACRP30
Nat. Med.
(2002) - et al.
The fat-derived hormone adiponectin reverses insulin resistance associated with both lipoatrophy and obesity
Nat. Med.
(2001)