Antioxidant Treatment Promotes Prostate Epithelial Proliferation in Nkx3.1 Mutant Mice
Figure 1
Nkx3.1−/− mouse prostate shows dysregulation of oxidative stress genes and increased oxidative stress levels.
(A) Quantitative reverse transcriptase-PCR analysis of RNA from 10–11-week and 16–17-week-old Nkx3.1+/+ and Nkx3.1−/− mouse anterior prostate for the expression of Gpx2, Prdx6, and Qsox1. Expression levels are relative to 18s rRNA. (10–11 weeks: n = 4 Nkx3.1+/+, n = 2 Nkx3.1−/−; 16–17 weeks: n = 3 Nkx3.1+/+, n = 5 Nkx3.1−/−) (B) ChIP-seq screen shots from Integrative Genomics Viewer (IGV) displays direct binding of Nkx3.1 to the gene loci of Gpx2, Prdx6 and Qsox1 in mouse prostate, (C) and to GPX2, PRDX6 and QSOX1 in the human prostate cancer cell line LNCaP. (D) ChIP-qPCR analysis for Nkx3.1 binding sites in GPX2, PRDX6, and QSOX1. Results are presented for each binding site primer set with anti-NKX3.1 antibody and IgG control. Immunoprecipitated DNA was normalized to 1% input. (E) Percent positive stained anterior prostate epithelial cells from immunohistochemical staining for 8-OHdG in one-year-old Nkx3.1+/+ and Nkx3.1−/− anterior prostate. (n = 5 in each group) Student's t-Test * = p≤0.05.