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Formation of the vascular system of developing bean (Phaseolus limensis L.) seeds according to nuclear magnetic resonance microtomography

  • Plant Developmental Biology
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Abstract

1H magnetic resonance microtomography imaging was applied to study vascular systems in developing bean (Phaseolus limensis L.) seeds. Using the gradient echo method, we recorded 2D tomographic sections in the sagittal and axial planes of the fruits sampled from a vegetating plant on days 10, 17, 24, and 31 after fertilization. Any vascular connection between the tissues of maternal plant (bean pod and seed coat) and the embryo were undetectable. The embryo has an autonomous branched network of procambial strands in the cotyledons, converging to the embryonic axis. The bean pods are covered with a network of vascular bundles; large vascular strands run along the dorsal and ventral sutures. The seed coat vascular bundles are formed in the process of seed ripening and are represented by a developed vascular system multiply branching in the middle part of the ground parenchyma at the stage of physiological maturity. They are connected with the source of assimilates via the lateral pod veins and a large vascular bundle, entering the seed below the hilum via the placenta. Assimilates enter the external part of the seed coat, which contains no vascular bundles, via the funiculus vascular bundles and hilum tissue.

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Authors and Affiliations

  1. Siberian State Technological University, pr. Mira 82, Krasnoyarsk, 660049, Russia

    I. S. Vinogradova

  2. Kirenskii Institute of Physics, Krasnoyarsk Scientific Center, Siberian Branch, Russian Academy of Sciences, Akademgorodok, 660036, Russia

    O. V. Falaleev

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  1. I. S. Vinogradova
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  2. O. V. Falaleev
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Correspondence to I. S. Vinogradova.

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Original Russian Text © I.S. Vinogradova, O.V. Falaleev, 2012, published in Ontogenez, 2012, Vol. 43, No. 1, pp. 28–38.

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Vinogradova, I.S., Falaleev, O.V. Formation of the vascular system of developing bean (Phaseolus limensis L.) seeds according to nuclear magnetic resonance microtomography. Russ J Dev Biol 43, 25–34 (2012). https://doi.org/10.1134/S1062360412010079

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  • DOI: https://doi.org/10.1134/S1062360412010079

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