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Influence of culture medium osmolality on maturation and ovulation of common frog oocytes stimulated in vitro by pituitary extract or progesterone

  • Gametogenesis
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Abstract

The influence of diluted Ringer solution on ovulation and maturation of common frog oocytes stimulated in vitro by homologous pituitary extract (0.005 pit/ml) or progesterone (1 μg/ml) was studied. During hibernation, the dilution of Ringer solution led to a decreased percentage of oocytes ovulated and matured under the influence of both inducers. As the season of reproduction approached, the dependence of oocyte maturation and ovulation on the Ringer solution dilution was reduced. Possible causes of different dependence of the ovulation of amphibian and sturgeon oocytes stimulated by gonadotropic hormones or progesterone on the culture medium osmolality is discussed.

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References

  • Behrman, H.R and Romero, R.J., Prostaglandins, Prostaglandin-Like Products in Reproduction: Eicosanoids, Peroxide, and Oxygenous Radicals, Reproductive Endocrinology, Jaffe, Y., Ed., Philadelphia: W.B. Saunders Co, 1991, pp. 238–272.

    Google Scholar 

  • Burgers, A.C. and Li, C.H., Amphibian Ovulation in vitro Induced by Mammalian Pituitary Hormones and Progesterone, Endocrinology, 1960, vol. 66, pp. 255–259.

    Article  CAS  Google Scholar 

  • Chang, K.J., Kim, J.W., Lee, J., et al., Prostaglandin Production and Ovulation during Exposure of Amphibian Ovarian Follicles to Gonadotropin or Phorbol Ester in vitro, Gen. Comp. Endocrinol., 1995, vol. 100, pp. 257–266.

    Article  PubMed  CAS  Google Scholar 

  • Chang, K.J., Kim, J.W., Im, W.B., et al., Differential Effects of Gonadotropin and Orthovanadate on Oocyte Maturation, Ovulation, and Prostaglandin Synthesis by Rana Ovarian Follicles in vitro, J. Exp. Zool., 1997, vol. 277, pp. 155–165.

    Article  PubMed  CAS  Google Scholar 

  • Goncharov, B.F. and Skoblina, M.N., Sturgeon Artificial Reproduction and in vitro Ovarian Follicles Manipulation, 1st Internat. Workshop on Biology, Conservation and Sustainable Development of Sturgeons in Southern Europe, Granada, 2005, p. 29.

  • Goncharov, B.F., Villot, P., and Menn, F., Morphological and Physiological Characteristics of Siberian Sturgeon Ovarian Follicles and Their Significance for Prognostication of Artificial Reproduction Success, Ontogenez, 1999, vol. 30, no. 1, pp. 51–60.

    Google Scholar 

  • Inazu, N. and Fujii, T., Ovarian Carbonyl Reductase Activity in Rats at Persist Oestrous, J. Reprod. Fertil., 1996, vol. 108, no. 1, pp. 123–130.

    Article  PubMed  CAS  Google Scholar 

  • Lundgren, D.W., Moore, R.M., Collins, P.L., et al., Hypotonic Stress Increases Cyclooxygenase-2 Expression and Prostaglandin Release from Amnion-Derived WISH Cells, J. Biol. Chem., 1997, vol. 272, no. 32, pp. 20118–20124.

    Article  PubMed  CAS  Google Scholar 

  • Masui, Y. and Clarke, H.J., Oocyte Maturation, Int. Rev. Cytol., 1979, vol. 57, pp. 185–282.

    Article  PubMed  CAS  Google Scholar 

  • Murdoch, W.J. and Farris, M.L., Prostaglandin E2-9-Ketoreductase Activity of Preovulatory Ovine Follicles, J. Anim. Sci., 1988, vol. 66, no. 11, pp. 2924–2929.

    PubMed  CAS  Google Scholar 

  • Schlegel, W., Kruger, S., and Korte, K., Purification of Prostaglandin E2-9-Oxoreductase from Human Decidua Vera, FEBS Lett., 1984, vol. 171, no. 1, pp. 141–144.

    Article  PubMed  CAS  Google Scholar 

  • Schuetz, A.W. and Glad, R., In vitro Production of Meiosis Inducing Substance (MIS) by Isolated Amphibian (Rana pipiens) Follicle Cells, Devel. Growth Differ., 1985, vol. 27, pp. 201–211.

    Article  CAS  Google Scholar 

  • Schuetz, A.W., Hormonal Dissociation of Ovulation and Maturation of Oocytes: Ovulation of Immature Amphibian Oocytes by Prostaglandin, Gamete Res., 1986, vol. 15, pp. 99–113.

    Article  CAS  Google Scholar 

  • Skoblina, M.N., Kondrat’eva, O.T., Nikiforova, G.P., et al., Effects of Chloride Channels and Media with Reduced Chlorine Ion Concentration on Ovulation of Common Frog Oocytes, Ontogenez, 1996, vol. 27, pp. 434–439.

    PubMed  CAS  Google Scholar 

  • Skoblina, M.N., Kondrat’eva, O.T., Nikiforova, G.P., et al., Role of Eicosanoids and Progesterone in Ovulation of Common Frog Oocytes, Ontogenez, 1997, vol. 28, pp. 211–216.

    PubMed  CAS  Google Scholar 

  • Snyder, B.W. and Schuetz, A.W., In vitro Evidence of Steroidogenesis in Amphibian (Rana pipiens) Ovarian Follicle and Its Relationship to Meiotic Maturation and Ovulation, J. Exp. Zool., 1973, vol. 183, pp. 333–342.

    Article  PubMed  CAS  Google Scholar 

  • Wright, P.A., Induction of Ovulation in Rana pipiens with Steroids, Gen. Comp. Endocrinol., 1961, vol. 1, pp. 20–23.

    Article  PubMed  CAS  Google Scholar 

  • Zhang, F., Warskulat, U., Wettstein, M., et al., Hyperosmolarity Stimulates Prostaglandin Synthesis and Cyclooxygenase-2 Expression in Activated Rat Liver Macrophages, Biochem. J., 1995, vol. 312, pp. 135–143.

    PubMed  CAS  Google Scholar 

  • Zhang Ming-Zhi, Hao Chuan-Ming, Breyer, M.D., et al., Mineralocorticoid Regulation of Cyclooxygenase-2 Expression in Rat Renal Medulla, Am. J. Renal Physiol., 2002, vol. 283, pp. F509–F516.

    CAS  Google Scholar 

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Original Russian Text © M.N. Skoblina, 2006, published in Ontogenez, 2006, Vol. 37, No. 6, pp. 457–464.

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Skoblina, M.N. Influence of culture medium osmolality on maturation and ovulation of common frog oocytes stimulated in vitro by pituitary extract or progesterone. Russ J Dev Biol 37, 382–388 (2006). https://doi.org/10.1134/S1062360406060075

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  • DOI: https://doi.org/10.1134/S1062360406060075

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