Skip to main content
Log in

Isolation and characterization of 15 microsatellite loci for Ophicephalus argus cantor

  • Short Communications
  • Published:
Russian Journal of Genetics Aims and scope Submit manuscript

Abstract

The isolation and development of 15 polymorphic dinucleotide microsatellite loci were described for Ophicephalus argus from the Huaihe River. All loci were polymorphic in the 30 individuals tested. The number of alleles per variable locus ranged from nine to seventeen, with a mean of 12.00. These novel microsatellite loci showed high level of polymorphism. Observed and expected heterozygosities ranged from 0.793 to 0.929 and from 0.841 to 0.952, respectively. Two loci were found deviated from HWE in the sampled population after Bonferroni correction. These microsatellite loci will be useful for revealing population structure, genetic diversity, and phylogeography of Ophicephalus argus.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

References

  1. Chu, X.L. and Chen, Y.R., Fishes of Yunnan Province II, Beijing: Science Press, 1990, pp. 264–266.

    Google Scholar 

  2. Ishimatsu, A. and Itazawa, Y., Difference in blood oxygen levels in the outflow vessels of the heart of an airbreathing fish, Channa argus: do separate blood streams exist in a Teleostean heart?, J. Comp. Physiol., 1983, vol. 149, pp. 435–440.

    Article  Google Scholar 

  3. Nelson, J.S., Fishes of the World, Hoboken: Wiley, 2006, 4th ed.

    Google Scholar 

  4. Wu, L.F., Zhang, D.M., Huang, Q., and Liu, C.L., The food analysis, population resources and utilization of snakehead fish (Ophiocephelus argus) in Huanghua Lake., J. Jilin Agric. Univ., 2000, vol. 22, no. 3, pp. 104–106.

    Google Scholar 

  5. Yu, H.Y., Ou, Y.S., Wu, X.P., et al., Studies on age and growth of Ophiocephalus argus in Poyang Lake, Life Sci. Res., 2008, vol. 12, no. 1, pp. 91–94.

    Google Scholar 

  6. Xiao, M.S., Cui, F., Kang, J., and Zhang, X.H., Genetic structure and variation of wild Ophiocephalus argus Cantor from Huaihe River based on mtDNA D-loop sequences, J. Huazhong Normal Univ. (Nat. Sci.), 2013, vol. 47, no. 1, pp. 82–90.

    CAS  Google Scholar 

  7. Wu, X.W., Economy Fauna of China, Beijing: Science Press, 1979.

    Google Scholar 

  8. Lu, J.Q. and Li, Z.H., Studies on the skeletal system of the Ophiocephalus argus Cantor, J. Henan Normal Univ. (Nat. Sci.), 1989, vol. 3, pp. 53–63.

    Google Scholar 

  9. Li, S.W. and Yang, H.Q., Status and prospect of Ophiocephalus argus Cantor culture in China, Mod. Fish. Inform., 1998, vol. 13, no. 11, pp. 6–8.

    Google Scholar 

  10. Li, J.W., Biological characteristics and artificial culture techniques of Ophiocephalus argus, China Fish., 1999, vol. 10, pp. 28–31.

    Google Scholar 

  11. Zhang, X.Q., Gong, S.Y., Liu, J., et al., Studies on age and growth of Channa argus in the Wanghu Lake, Acta Hydrobiol. Sin., 1999, vol. 23, no. 6, pp. 600–603.

    Google Scholar 

  12. Qiao, X.T., Zhang, M.T., Song, X.J., et al., Study on proteinase activity of Ophiocephalus argus, Acta Sci. Nat. Univ. Nankaiensis (Nat. Sci. Ed.), 1999, vol. 32, no. 2, pp. 121–123.

    Google Scholar 

  13. Qin, W., Ni, J.G., Zhang, X.B., and Zhou, J.F., Comparative studies on chromosomes in the snakehead fish Channa argus and Ophiocephalidae, Reservoir Fish., 2004, vol. 24, no. 5, pp. 14–16.

    Google Scholar 

  14. Yin, W.L. and Dai, J.H., Studies on the isozymes of different tissues in Ophiocephalus argus, J. Hubei Normal Univ. (Nat. Sci.), 2000, vol. 20, no. 3, pp. 17–20.

    Google Scholar 

  15. Li, X., Ou, Y.S., Wu, X.P., et al., Studies on the EST and LDH isozymes in different tissues of Ophiocephalus argus in Poyang Lake, Hubei Agric. Sci., 2010, vol. 49, no. 1, pp. 153–155.

    Google Scholar 

  16. Wright, J. and Bentzen, P., Microsatellites: genetic markers for the future, Fish Biol. Fish., 1994, vol. 4, no. 3, pp. 384–388.

    Article  Google Scholar 

  17. Refseth, U.H., Fangan, B.M., and Jakobsen, K.S., Hybridization capture of microsatellites directly from genomic DNA, Electrophoresis, 1997, vol. 18, pp. 1519–1523.

    Article  CAS  PubMed  Google Scholar 

  18. Glenn, T.C. and Schable, N.A., Isolating microsatellite DNA loci, Methods Enzymol., 2005, vol. 395, pp. 202–222.

    Article  CAS  PubMed  Google Scholar 

  19. Zane, L., Bargelloni, L., and Patarnello, T., Strategies for microsatellite isolation: a review, Mol. Ecol., 2002, vol. 11, pp. 11–16.

    Article  Google Scholar 

  20. Clarke, K.R. and Gorley, R.N., PRIMER v5: User Manual/Tutorial, Plymouth: PRIMER-E, 2001.

    Google Scholar 

  21. Schuelke, M., An economic method for the fluorescent labeling of PCR fragments, Nat. Biotechnol., 2000, vol. 18, pp. 233–234.

    Article  CAS  PubMed  Google Scholar 

  22. Sambrook, J. and Russel, D.W., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor: Cold Spring Harbor Lab. Press, 2001, 3rd ed.

    Google Scholar 

  23. Marshall, T.C., Slate, J., Kruuk, L.E.B., and Pemberton, J.M., Statistical confidence for likelihood-based paternity inference in natural populations, Mol. Ecol., 1998, vol. 7, pp. 639–665.

    Article  CAS  PubMed  Google Scholar 

  24. Raymond, M. and Rousset, F., Genepop (version 3.4): population genetics software for exact tests and ecumenicism, J. Hered., 1995, vol. 86, pp. 248–249. http://wbiomed.curtin.edu.au/genepop/.html

    Google Scholar 

  25. Rivera, M.A.J., Gralmm, G.C., and Roderick, G., Isolation and characterization of nine microsatellite loci from the Hawaiian Grouper Epinephelus quernus (Serraindae) for population genetic analyses, Mar. Biotechnol., 2003, vol. 5, pp. l–26.

    Article  Google Scholar 

  26. van Oosterhout, C., Hutchinson, W.F., Wills, D.P.M., and Shipley, P., Micro-checker: software for identifying and correcting genotyping errors in microsatellite data, Mol. Ecol. Notes, 2004, vol. 4, pp. 535–538.

    Article  CAS  Google Scholar 

  27. Sun, Y., Lin, H.D., Tang, W.Q., et al., Polymorphic microsatellite loci isolated from the Squalidus argentatus using PCR-based isolation of microsatellite frrays (PIMA), Int. J. Mol. Sci., 2011, vol. 12, no. 9, pp. 5666–5671.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to M. Xiao.

Additional information

The article is published in the original.

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Xiao, M., Xia, H. & Bao, F. Isolation and characterization of 15 microsatellite loci for Ophicephalus argus cantor. Russ J Genet 51, 1044–1047 (2015). https://doi.org/10.1134/S1022795415090136

Download citation

  • Received:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1134/S1022795415090136

Keywords

Navigation