Abstract
The light-induced action of 8-methoxypsoralen (8-MOP) on λ phage and plasmids yields monoadducts and interstrand crosslinks. The survival and clear plaque mutation frequency in the phage photosensitized with 8-MOP and irradiated with UV at wavelength >320 nm are increased when the wild-type host (Escherichia coli uvr +) is subjected to UV irradiation (wavelength = 254 nm) prior to phage inoculation. These phenomena are known as “W reactivation” and “W mutagenesis.” It is shown that 8-MOP monoadducts in λ DNA induce clear mutations in the phage inoculated to UV-irradiated excision repair mutants of E. coli only when the error-prone repair is performed by MucA ′2 B, but not PolV (UmuD ′2 C) polymerase. The efficiency of the SOS repair (W reactivation) of 8-MOP monoadducts in plasmid and λ phage DNA also only increases with the presence of pKM101 plasmid muc + in E. coli uvr −.
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Original Russian Text © G.B. Zavil’gel’skii, V.Yu. Kotova, 2013, published in Genetika, 2013, Vol. 49, No. 12, pp. 1370–1375.
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Zavil’gel’skii, G.B., Kotova, V.Y. SOS repair of 8-methoxypsoralene monoadducts in DNA of lambda bacteriophage and plasmids is mediated by MucA ′2 B, but not UmuD ′2 C (PolV) polymerase. Russ J Genet 49, 1195–1199 (2013). https://doi.org/10.1134/S1022795413120144
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DOI: https://doi.org/10.1134/S1022795413120144