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Molecular characteristics of the SLA-2 gene from Chinese Hebao pigs

  • Animal Genetics
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Abstract

To study the molecular characteristics of swine leukocyte antigen (SLA) class I from the Hebao pig, a rare inbreed in China, a pair of primers was designed to amplify the SLA-2 gene (SLA-2-HB) and then the molecular characteristics of the gene were analyzed by computer. After cloning, sequencing and computer analysis, four SLA-2-HB alleles were found, all of 1119 bp. Sites 3–1097 were an open reading frame encoding 364 amino acids with two sets of intra-chain disulfide bonds comprising four cysteines situated in sites 125, 188, 227 and 283. By alignment of SLA-2-HB sequences with other SLA-2 alleles in the DNA Data Bank of Japan/European Molecular Biology Laboratory/GenBank database, nine key variable amino acid sites were found in the extracellular domain of the SLA-2-HB alleles at sites 23(F), 24(I), 43(A), 44(K), 50(Q), 73(N), 95(I), 114(R) and 216(S), which could be used to differentiate other SLA-2 alleles. The amino acid identities between SLA-2-HB and other SLA-2, SLA-3 and SLA-1 alleles were 87.1–97.0%, 85.0–93.9% and 83.3–88.6%, respectively. The phylogenetic tree of SLA-2-HB showed that it was relatively independent of the other SLA-2 genes. Furthermore, the SLA-2-HB alleles were similar to HLA-B15 and HLA-A2 functional domains and preserved some functional sites of HLA-A2. It was concluded that SLA-2-HB is an allele of SLA-2 and that the Hebao pig might have evolved independently in China.

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Authors and Affiliations

  1. Department of Biochemistry and Molecular Biology, College of Bioengineering, Dalian University, Dalian, Liaoning, 116622, China

    F. -Sh. Gao & J. Bai

  2. Institute of Animal Husbandry of Liaoning Province, Liaoyang, Liaoning, 111000, China

    X. -H. Zhang, W. -J. Zhang, D. Guo & Sh. Zhang

Authors
  1. F. -Sh. Gao
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  2. J. Bai
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  3. X. -H. Zhang
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  4. W. -J. Zhang
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  5. D. Guo
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  6. Sh. Zhang
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Correspondence to F. -Sh. Gao.

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Gao, F.S., Bai, J., Zhang, X.H. et al. Molecular characteristics of the SLA-2 gene from Chinese Hebao pigs. Russ J Genet 48, 224–229 (2012). https://doi.org/10.1134/S1022795412010073

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  • DOI: https://doi.org/10.1134/S1022795412010073

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