Abstract
Using five microsatellite loci, genotyping and genetic diversity estimates were obtained for nine samples representing seven common carp breeds most widespread in Russia. For comparison, the samples of Amur wild common carp (Cyprinus carpio haematopterus) and a sample of European Hungarian carp were used. In the samples examined (n = 148) a total of 78 alleles were revealed. The highest mean allele number per locus (7.3) was identified in Amur wild common carp, while the lowest number was found in Cherepets carps (4.0). In different breeds, the observed heterozygosities varied from 0.819 (Altai carp) to 0.651 (Cherepets scaly carp). Three out of five microsatellite loci (MFW-24, MFW-28, and MFW-19) revealed a high level of population differentiation. In the dendrogram of genetic differences, all breeds clustered into two groups. One of these groups was composed of the two strains of Ropsha carp, Stavropol carp, Amur wild common carp, and the two samples of Cherepets carp. The second cluster included Altai carp (Priobskii and Chumysh populations), two Angelinskii carp breeds (mirror and scaly), and Hungarian carp. The pairs of breeds/populations/strains, having common origin, were differentiated. Specifically, these were two populations of Altai carp, two strains of Ropsha carp, as well as the breeds of Angelinskii and Cherepets carps. The reasons for genetic differentiation of Russian common carp breeds, as well as the concordance of the evolutionary histories of these breeds, some of which originated from the European breeds, while the others contain substantial admixture of the Amur wild common carp, are discussed.
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References
Ellegren, H., Microsatellites: Simple Sequences with Complex Evolution, Nat. Rev. Genet., 2004, vol. 5, pp. 435–445.
Chistiakov, D.A., Hellemans, B., and Volckaert, F.A.M., Microsatellites and Their Genomic Distribution, Evolution, Function and Applications: A Review with Special Reference to Fish Genetics, Aquaculture, 2006, vol. 255, pp. 1–29.
Shimoda, N., Knapic, E.W., Ziniti, J., et al., Zebrafish Genetic Map with 2000 Microsatellite Markers, Genomics, 1999, vol. 58, pp. 219–232.
Zardoya, R. and Doadrio, I., Molecular Evidence on the Evolutionary and Biogeographical Patterns of European Cyprinids, J. Mol. Evol., 1999, vol. 49, pp. 227–237.
Balon, E.K., Origin and Domestication of the Wild Carp, Cyprinus carpio—from Roman Gourmets to the Swimming Flomers, Aquaculture, 1995, vol. 129, pp. 3–48.
Orban, L. and Wu, Q., Cyprinids, in Genome Mapping and Genomics in Fishes and Aquatic Animals, Berlin: Springer-Verlag, 2008, vol. 2, pp. 45–83.
Crooijmans, R.P., Bierbooms, V.A.F., Komen, J., et al., Microsatellite Markers in Common Carp (Cyprinus carpio L.), Anim. Genet., 1997, vol. 28, pp. 129–134.
Aliah, R.S., Tagaki, M., Teoh, C.T., and Taniguchi, N., Isolation and Inheritance of Microsatellite Markers in the Common Carp Cyprinus carpio, Fish Sci., 1999, vol. 65, pp. 235–239.
Tanck, M.W.T., Baars, H.C.A., Kohlmann, K., et al., Genetic Characterization of Wild Dutch Common Carp (Cyprinus carpio L.), Aq. Res., 2001, vol. 31, no. 10, pp. 779–783.
David, L., Rajasekaran, P., Fang, J., et al., Polymorphism in Ornamental and Common Carp Strains (Cyprinus carpio L.) as Revealed by RFLP Analysis and a New Set of Microsatellite Markers, Mol. Gen. Genet., 2001, vol. 266, pp. 353–362.
Kohlmann, K., Gross, R., Murakaeva, A., and Kersten, P., Genetic Variability and Structure of Common Carp (Cyprinus carpio) Populations throughout the Distribution Rang Inferred from Allozyme, Microsatellite and Mitochondrial DNA Markers, Aq. Liv. Res., 2003, vol. 16, pp. 421–431.
Bartfay, R., Egedi, S., Yue, G., et al., Genetic Analysis of Two Common Carp Broodstocks by RAPD and Microsatellite Markers, Aquaculture, 2003, vol. 219, pp. 157–167.
Zhou, J., Wu, Q., Wang, Z., and Ye, Y., Genetic Variation Analysis within and among Six Varieties of Common Carp (Cyprinus carpio L.) in China Using Micro-satellite Markers, Russ. J. Genet., 2004, vol. 40, pp. 1389–1393.
Jewel, M.A., Rahman, M.M., and Islam, M.N., Study of Genetic Variation in Different Hatchery Populations of Common Carp (Cyprinus caprio) of Mimensight District in Bangladesh Using Microsatellite DNA Markers, J. Bio-Sci., 2006, vol. 14, pp. 113–120.
Mondol, S.K., Islam, S., and Alam, S., Characterization of Different Strains of Common Carp (Cyprinus carpio L.) (Cyprinidae, Cyprinoformes) in Bangladesh Using Microsatellite DNA Markers, Gen. Mol. Biol., 2006, vol. 29, 4, pp. 626–633.
Thai, B.T., Burridge, C.P., and Austin, C.M., Genetic Diversity of Common Carp (Cyprinus carpio L.) in Vietnam Using Four Microsatellite Loci, Aquaculture, 2007, vol. 269, no. 1–4, pp. 174–186.
Yue, G.H. and Orban, L., Polymorphic Microsatellites from Silver Crucian Carp (Carassius auratus gibelo Bloch) and Crossamplification in Common Carp (Cyprinus carpio L.), Mol. Ecol. Not., 2002, vol. 2, pp. 534–536.
Yue, G.H., Ho, M.Y., Orban, L., and Komen, J., Microsatellites within Genes EST’s of Common Carp and Their Applicability in Silver Crucian Carp, Aquaculture, 2004, vol. 234, pp. 85–98.
Ludanny, R.I., Chrisanfova, G.G., Vasil’ev, V.A., et al., Genetic Diversity and Differentiation of Russian Common Carp (Cyprinus carpio L.) Breeds Inferred from RAPD Markers, Russ. J. Genet., 2006, vol. 42, no. 8, pp. 928–935.
Lewis, P.Q. and Zaykin, D., Genetic Data Analysis: Computer Program for the Analysis of Allelic Data. Version 1.0 (d12), Free Program Distributed by Authors, 2000, http://lewis.eeb.uconn.edu/lewishome/soft-ware.htlm/
Nei, M., Estimation of Average Heterozygosity and Genetic Distance from a Small Number of Individuals, Genetics, 1978, vol. 89, pp. 583–590.
Goldstein, D.B., Linares, A.R., Cavalli-Sforza, L.L., and Feldman, M.W., An Evaluation of Genetic Distances for Use with Microsatellite Loci, Genetics, 1994, vol. 139, pp. 463–471.
Katalog porod, krossov i odomashnennykh form ryb Rossii i SNG (Catalog of Breeds, Crosses, and Domesticated Fish Forms of Russia and CIS), Bogeruk, A.K., Ed., Moscow: Ministerstvo sel’skogo khozaystva, 2001.
Kirpitchnikov, V.S., Genetics and Breeding of Common Carp (Cyprinus carpio L.), Paris: INRA, 1999.
Semenova, S.K., Chrisanfova, G.G., Ludanny, R.I., et al., Patent no. 2294633.
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Original Russian Text © R.I. Ludanny, G.G. Chrisanfova, V.K. Prizenko, A.K. Bogeruk, S.K. Semyenova, 2010, published in Genetika, 2010, Vol. 46, No. 5, pp. 652–658.
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Ludanny, R.I., Chrisanfova, G.G., Prizenko, V.K. et al. Polymorphism of microsatellite markers in Russian common carp (Cyprinus carpio L.) breeds. Russ J Genet 46, 572–577 (2010). https://doi.org/10.1134/S1022795410050108
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DOI: https://doi.org/10.1134/S1022795410050108