Abstract
A total of 40 introgression lines of common wheat (2 n = 42) Triticum aestivum L × T. timopheevii Zhuk., resistant to leaf rust and partly to powdery mildew, were examined. Based on cytological analysis of meiosis in pollen mother cells (PMC), hybrid lines were subdivided into two groups characterized by either stable or unstable meiosis. In cytologically stable lines, chromosome configuration at the MI stage of meiosis was mostly bivalent (21II) with small proportion of defect cells (almost 10%), which at most contained two univalents (20II + 2I). Cytologically unstable group was comprised of the lines, containing high proportions of cells with abnormal chromosome pairing in meiotic PMC, as well as the cells with multivalents, and the lines containing aneuploid plants. Localization of the T. timopheevii fragments performed with the use of SSR markers showed that the lines with unstable meiosis were characterized by higher numbers of introgressions compared to stable lines. The influence of certain chromosomes of T. timopheevii on chromosome pairing stability was also demonstrated. In cytologically unstable lines, the increased frequency of 2A substitutions along with the high frequency of introgression of T. timopheevii genetic material into chromosome 7A was observed. Multivalents were scored in all cases of introgression in chromosome 7A. It was suggested that the reason for the genome instability in hybrid forms lied in insufficient compensating ability of certain T. timopheevii chromosomes and/or their parts, involved into recombination processes.
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Haijar, R. and Hodgkin, T., The Use of Wild Relatives in Crop Improvement: A Survey of Developments over the Last 20 Years, Euphytica, 2007, vol. 156, pp. 1–13.
Friede, B., Jang, J., Raupp, W.J., et al., Characterization of Wheat-Alien Translocations Conferring Resistance to Diseases and Pests: Current Status, Euphytica, 1996, vol. 91, pp. 59–87.
McIntosh, R.A., Yamazaki, Y., Devos, K.M., et al., Catalogue of Gene Symbols for Wheat, Proseedings of the 10th International Wheat Genetics Symposium, Pogna, N.E., Romano, M., Pogna, E.A. and Galterio, G., Eds., Rome: Inst. Sperimentale per la Cerelicoltura, 2003, vol. 4, pp. 1–34.
Budashkina, E.B., Cytogenetic Study of introgression Disease Resistant Common Wheat Lines, Tag. Ber. Akad. Landwirtschaft Wissenschaft DDR, 1988, vol. 206, pp. 209–221.
Jiang, J. and Gill, B.S., Different Species-Specific Chromosome Translocation in Triticum timopheevii and T. turgidum Support Diphyletic Origin of Polyploid Wheats, Chromosome, 1994, res. 2, pp. 59–64.
Feldman, M., Origin of Cultivated Wheat, Bonjean A.P., Angus W.J., Eds., in The World Wheat Book, Paris: Lavoisier, 2001.
Feldman, M., Identification of Unpaired Chromosomes in F1 Hybrids Involving T. aestivum and T. timophevii, Can. J. Genet. Cytol., 1966, vol. 8, pp. 144–151.
Shkutina, F.M., Kalinina, N.P., and Usova, T.K., Role of a Soft Wheat Variety in Introgression of Alien Genetic Material in Its Genome and the Stabilization Rate of a Hybrid Form, Genetika (Moscow), 1988, vol. 24, no. 1, pp. 98–109.
Gupta, P.K., Balyan, H.S., Sharma, P.C., and Ramesh, B., Microsatellites in Plants: A New Class of Molecular Markers, Curr. Sci., 1996, vol. 70, pp. 45–54.
Röder, M.S., Plaschke, J., König, S.U., et al., Abundance, Variability and Chromosome Location of Microsatellites in Wheat, Mol. Gen. Genet., 1995, vol. 246, pp. 327–333.
Khlestkina, E.K., Röder, M.S., Efremova, T.T., et al., The Genetic Diversity of Old and Modern Siberian Varieties of Common Spring Wheat as Determined by Microsatellite Markers, Plant Breed., 2004, vol. 123, pp. 122–127.
Leonova, I.N., Laikova, L.I., Popova, O.M., et al., Detection of Quantitative Trait Loci for Leaf Rust Resistance in Wheat — T. timopheevii/T. tauschii Introgression Lines, Euphytica, 2007, pp. 79–86.
Kalinina, N.P., Cherkasova, M.V., and Budashkina, E.B., Malat Dehydrogenase as a Genetic Marker in the Analysis of Interspecies Wheat Hybrids (T. aestivum × T. timopheevii), Genetika (Moscow), 1987, vol. 23, no. 7, pp. 1240–1246.
Badaeva, E.D., Budashkina, E.B., Badaev, N.S., et al., General Features of Chromosome Substitutions in T. aestivum × T. timopheevii Hybrids, Theor. Appl. Genet., 1991, vol. 82, pp. 227–232.
Leonova, I.N., Roder, M.S., Budashkina, E.B., et al., Molecular Analysis of Leaf-Rust-Resistance Introgression Lines Obtained by Crossing of Hexarloid Wheat Triticum aestivum with Tetraploid Wheat Triticum timopheevii, Russ. J. Genet., 2002, vol. 38, no. 12, pp. 1397–1403.
Kimber, G. and Huls, M.M., The Analysis of Chromosome Pairing in Hybrids and the Evolution of Wheat, Proc. 5th Int. Wheat Genet. Symp., Ramanujam, S., Ed., New Dehli, 1978, pp. 408–419.
Plaschke, J. and Roder, M.S., Detection of Diversity in Closely Related Bread Wheat Using Microsatellite Markers, Theor. Appl. Genet., 1995, vol. 91, pp. 1001–1007.
Röder, M.S., Korzun, V., Wendehake, K., et al., A Microsatellite Map of Wheat, Genetics, 1998, vol. 146, pp. 2007–2023.
Pestsova, E., Ganal, M.W., and Röder, M.S., Isolation and Mapping of Microsatellite Markers Specific for the D Genome of Bread Wheat, Genome, 2000, vol. 43, pp. 689–697.
Salina, E.A., Leonova, I.N., Efremova, T.T., and Roeder, M.S., Wheat Genome Structure: Translocations during the Course Polyploidization, Funct. Integr. Genomics, 2006, vol. 6, pp. 71–80.
Vasil’eva, L.A., Biologicheskaya statistika: Uchebn. posobie po kursu lektsii “Biometriya” (Biological Statistics: Biometry Handbook), Novosibirsk: ITsiG, NGU, 2000.
Badaeva, E.D., Prokofieva, Z.D., Bilinskaya, E.N., et al., Cytogenetic Analysis of Hybrids Resistant to Yellow Rust and Powdery Mildew Obtained by Crossing Common Wheat (Triticum aestivum L., AABBDD) with Wheats of the Timopheevi Group (AtAtGG), Russ. J. Genet., vol. 2000, vol. 36, no. 12, pp. 1401–1410.
Silkova, O.G., Dobrovolskaya, O.B., Dubovets, N.I., et al., Production of Wheat-Rye Substitution Lines and Identification of Chromosome Composition of Karyotypes Using S-Banding, GISH, and SSR Markers, Russ. J. Genet., 2006, vol. 42, no. 6, pp. 645–653.
Gaidalenok, R.F. and Maistrenko, O.I., The Study of MI Meiosis in F1 Hybrids from Crosses between Chinese Spring Monosomics and Cultivars without Chromosome Pairing Abnormalities, in Tsitogeneticheskie issledovaniya aneuploidov myagkoi pshenitsy (Cytogenetic Studies of Common Wheat Aneuploids), Novosibirsk: Izd. ITsiG, 1973, pp. 77–94.
Rodriguez, S., Perera, E., Maestra, B., et al., Chromosome Structure of Triticum timopheevii Relative to T. turgidum, Genome, 2000, vol. 43, pp. 923–930.
Tao, W., Liu, D., and Liu, J., Genetic Mapping of the Powdery Mildew Resistance Gene Pm6 in Wheat by RFLP Analysis, Theor. Appl. Genet., 2000, vol. 100, pp. 564–568.
Jäve, K., Peusha, H.O., Tsymbalova, J., et al., Chromosomal Location of a Triticum timopheevii—Derived Powdery Mildew Resistance Gene Transferred to Common Wheat, Genome, 2000, vol. 43, no. 2, pp. 377–381.
leaf-Guedira, G.L., Singh, S., and Fritz, A.K., Performance and Mapping of Leaf Rust Resistance Transferred to Wheat from Triticum timopheevii subsp. armeniacum, Phytopathology, 2003, vol. 93, pp. 784–789.
Leonova, I., Börner, A., Budashkina, E., et al., Identification of Microsatellite Markers for a Leaf Rust Resistance Gene Introgressed into Common Wheat from T. timopheevii, Plant Breed., 2004, vol. 123, pp. 93–95.
Leonova, I.N., Laikova, L.I., Popova, O.M., et al., Detection of Quantitative Trait Loci for Leaf Rust Resistance in Wheat-T. timopheevii/T. tauschii Introgression Lines, Euphytica, 2007, vol. 155, pp. 79–86.
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Original Russian Text © E.I. Gordeeva, I.N. Leonova, N.P. Kalinina, E.A. Salina, E.B. Budashkina, 2009, published in Genetika, 2009, Vol. 45, No. 12, pp. 1616–1626.
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Gordeeva, E.I., Leonova, I.N., Kalinina, N.P. et al. Comparative cytological and molecular analysis of common wheat introgression lines containing genetic material of Triticum timopheevii Zhuk. Russ J Genet 45, 1428–1437 (2009). https://doi.org/10.1134/S1022795409120047
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DOI: https://doi.org/10.1134/S1022795409120047