Abstract
Gene vfr of Pseudomonas chlororaphis 449 previously described only in Pseudomonas aeruginosa was identified, cloned, and sequenced; its localization in the chromosome was determined. Amino acid sequence of the protein encoded by gene vfr in P. chlororaphis 449 was shown to have a 83% identity with the Vfr protein of P. aeruginosa PAO1 and a 63% identity with the CRP protein of Escherichia coli. Amino acid residues that ensure the most important structural properties of the CRP protein, i.e., its binding to cAMP, RNA polymerase, and DNA, were identical or highly conserved in Vfr proteins of P. aeruginosa and P. chlororaphis 449. The cloned vfr gene of P. chlororaphis 449 was complemented partially the mutation at gene crp in cells of E. coli AM306 enhancing ten times synthesis of β-galactosidase dependent on the CRP protein. Unlike P. aeruginosa, the Vfr protein in cells of P. chlororaphis 449 does not participate in the regulation of synthesis of N-acyl-homoserine lactones.
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References
Waters, C. and Bassler, B., Quorum Sensing: Cell-to-Cell Communication in Bacteria, Annu. Rev. Cell Dev. Biol., 2005, vol. 21, pp. 319–346.
Khmel, I.A. and Metlitskaya, A.Z., Quorum Sensing Regulation of Gene Expression: A Promising Target for Drugs against Bacterial Pathogenicity, Mol. Biol. (Moscow), 2006, vol. 40, pp. 195–210.
Khmel, I.A., Quorum Sensing Regulation of Gene Expression: Fundamental and Applied Aspects and the Role in Bacterial Communication, Mikrobiologiya, 2006, vol. 75, pp. 457–464.
Venturi, V., Regulation of Quorum Sensing in Pseudomonas, FEMS Microbiol. Rev., 2006, vol. 30, pp. 274–291.
West, S.E.H., Sample, A.K., and Runyen-Janecky, L.J., The vfr Gene Product, Required for Pseudomonas aeruginosa Exotoxin A and Protease Production, Belongs to the Cyclic AMP Receptor Protein Family, J. Bacteriol., 1994, vol. 176, pp. 7532–7542.
Albus, A.M., Pesci, E.C., Runyen-Janecky, L.J., et al., Vfr Controls Quorum Sensing in Pseudomonas aeruginosa, J. Bacteriol., 1997, vol. 179, pp. 3928–3935.
Beatson, S.A., Whitchurch, C.B., Sargent, J.L., et al., Differential Regulation of Twitching Motility and Elastase Production by Vfr in Pseudomonas aeruginosa, J. Bacteriol., 2002, vol. 184, pp. 3605–3613.
Medina, G., Juarez, K., Valderrama, B., and Soberon-Chavez, G., Mechanism of Pseudomonas aeruginosa Rh1R Transcriptional Regulation of the rhlAB Promoter, J. Bacteriol., 2003, vol. 185, pp. 5976–5983.
Suh, S.-J., Runyen-Janecky, L.J., Maleniak, T.C., et al., Effect of vfr Mutation on Global Gene Expression and Catabolite Repression Control of Pseudomonas aeruginosa, Microbiology, 2002, vol. 148, pp. 1561–1569.
Wood, D., Gong M., Daykin M. et al., N-Acyl-Homoserine Lactone-Mediated Regulation of Phenazine Gene Expression by Pseudomonas aureofaciens 30–84 in the Wheat Rhizosphere, J. Bacteriol., 1997, vol. 179, pp. 7663–7670.
Zhang, Z. and Pierson III, L.S., A Second Quorum-Sensing System Regulates Cell Surface Properties but not Phenazine Antibiotic Production in Pseudomonas aureofaciens, Appl. Environ. Microbiol., 2001, vol. 67, pp. 4305–4315.
Veselova, M.A., Klein, Sh., Bass, I.A., et al., Quorum Sensing Systems of Regulation, Synthesis of Phenazine Antibiotics, and Antifungal Activity in Rhizospheric Bacterium Pseudomonas chlororaphis 449, Russ. J. Genet., 2008, vol. 44, no. 12, pp. 1400–1408.
de Lorenzo, V., Herrero, M., Jakubzik, U., and Timmis, K., Mini-Tn5 Transposon Derivatives for Insertion Mutagenesis, Promoter Probing, and Chromosomal Insertion of Cloned DNA in Gram-Negative Eubacteria, J. Bacteriol., 1990, vol. 172, pp. 6568–6572.
McClean, K., Winson, M., Fish, L., et al., Quorum Sensing and Chromobacterium violaceum, Exploitation of Violacein Production and Inhibition for the Detection of N-Acylhomoserine Lactones, Microbiology, 1997, vol. 143, pp. 3703–3711.
Shaw, P., Ping, G., Daly, S., et al., Detecting and Characterizing N-Acyl-Homoserine Lactone Signal Molecules by Thin-Layer Chromatography, Proc. Natl. Acad. Sci. USA, 1997, vol. 94, pp. 6036–6041.
Hoang, T., Karkhoff-Schweizer, R., Kutchma, A., and Schweizer, H., A Broad-Host-Range Flp-FRT Recombination System for Site-Specific Excision of Chromosomally-Located DNA Sequences, Application for Isolation of Unmarked Pseudomonas aeruginosa Mutants, Gene, 1998, vol. 212, pp. 77–86.
Dykxhoorn, D., St. Pierre, R., and Linn, T., A Set of Compatible tac Promoter Expression Vectors, Gene, 1996, vol. 177, pp. 133–136.
Aiba, H., Fujimoto, S., and Ozaki, N., Molecular Cloning and Nucleotide Sequencing of the Gene for Escherichia coli cAMP Receptor Protein, Nucleic Acids Res., 1982, vol. 10, pp. 1345–1361.
Miller, J.H., Experiments in Molecular Genetics, Cold Spring Harbor: Cold Spring Harbor Lab., 1972.
Maniatis, T., Fritsch, E.F., and Sambrook, J., Molecular Cloning: A Laboratory Manual, Cold Spring Harbor, New York: Cold Spring Harbor Lab., 1989.
Schweizer, H., Allelic Exchange in Pseudomonas aeruginosa Using Novel ColE1-Type Vectors and a Family of Cassettes Containing a Portable oriT and the Counter-Selectable Bacillus subtilis sacB Marker, Mol. Microbiol., 1992, vol. 6, pp. 1195–1204.
Laemmli, U.K., Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4, Nature, 1970, vol. 227, pp. 680–685.
Wolfgang M.C., Lee V.T., Gilmore, M.E., and Lory, S., Coordinate Regulation of Bacterial Virulence Genes by a Novel Adenylate Cyclase-Dependent Signaling Pathway, Dev. Cell., 2003, vol. 4, pp. 253–263.
Kolb, A., Busby, S., Buc, H., et al., Transcriptional Regulation by cAMP and Its Receptor Protein, Annu. Rev. Biochem., 1993, vol. 62, pp. 749–795.
Kanack, K.J., Runyen-Janecky, L.J., Ferrell, E.P., et al., Characterization of DNA-Binding Specificity and Analysis of Binding Sites of the Pseudomonas aeruginosa Global Regulator, Vfr, a Homologue of the Escherichia coli cAMP Receptor Protein, Microbiology, 2006, vol. 152, pp. 3485–3496.
MacGregor, C.H., Arora, S.K., Hager, P.W., et al., The Nucleotide Sequence of the Pseudomonas aeruginosa pyrE-crc-rph Region and the Purification of the crc Gene Product, J. Bacteriol., 1996, vol. 178, pp. 5627–5635.
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Original Russian Text © M.A. Veselova, V.A. Lipasova, M.I. Ovadis, L.S. Chernin, I.A. Khmel’, 2009, published in Genetika, 2009, Vol. 45, No. 9, pp. 1203–1210.
An erratum to this article can be found online at http://dx.doi.org/10.1134/S1022795410020183.
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Veselova, M.A., Lipasova, V.A., Ovadis, M.I. et al. Structure and expression of gene vfr in Pseudomonas chlororaphis 449. Russ J Genet 45, 1055–1061 (2009). https://doi.org/10.1134/S1022795409090051
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DOI: https://doi.org/10.1134/S1022795409090051