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Gene yddG of Escherichia coli encoding the putative exporter of aromatic amino acids: Constitutive transcription and dependence of the expression on the cell growth rate

  • Genetics of Microorganisms
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Abstract

Gene yddG of Escherichia coli encodes a protein of the inner membrane. Data obtained earlier demonstrated that under conditions of aromatic amino acids overproduction YddG promotes their export from E. coli cells. In this work, a method of primer extension was used to localize the P yddG promoter, which corresponds to E. coli promoters recognized by RNA polymerase in complex with σ70 or σS subunits. By constructing a gene of the hybrid protein YddG’-LacZ at the intrinsic site of gene yddG location in the E. coli chromosome and analyzing the activity of β-galactosidase in cells growing on laboratory media LB and M9, the constitutive type of yddG expression at a low level was demonstrated (the activity was about 3 to 4% of the LacZ level under induction of the lac operon in E. coli wild-type cells). The expression of yddG had a twofold increase under conditions of retarded cell growth upon the stress caused by the high NaCl content (0.6 M) or by the presence of phenylalanine excess quantities (>1 mM) in the culture medium.

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References

  1. Eggeling, L. and Sahm, H., New Ubiquitous Translocators: Amino Acid Export by Corynebacterium and Escherichia coli, Arch. Microbiol., 2003, vol. 180, no. 3, pp. 155–160.

    Article  PubMed  CAS  Google Scholar 

  2. Zakataeva, N.P., Kutukova, E.A., Gronskii, S.V., et al., Export of Metabolites by the Proteins of the DMT and RhtB Families and Its Possible Role in Intercellular Communication, Mikrobiologiya, 2006, vol. 75, no. 4, pp. 509–520.

    CAS  Google Scholar 

  3. Van Dyk, T.K., Templeton, L.J., Cantera, K.A., et al., Characterization of the Escherichia coli AaeAB Efflux Pump: A Metabolic Relief Valve?, J. Bacteriol., 2004, vol. 186, no. 21, pp. 7196–7204.

    Article  PubMed  Google Scholar 

  4. Livshits, V.A., Zakataeva, N.P., Aleshin, V.V., and Vitushkina, M.V., Identification and Characterization of the New Gene RhtA Involved in Threonine and Homoserine Efflux in Escherichia coli, Res. Microbiol., 2003, vol. 154, pp. 123–135.

    Article  PubMed  CAS  Google Scholar 

  5. Kutukova, E.A., Livshits, V.A., Altman, I.P., et al., The yeaS (leuE) Gene of Escherichia coli Encodes an Exporter of Leucine, and the Lrp Protein Regulates Its Expression, FEBS Lett., 2005, vol. 579, pp. 4629–4634.

    Article  PubMed  CAS  Google Scholar 

  6. Daßler, T., Maier, T., Winterhalter, C., and Böck, A., Identification of a Major Facilitator Protein from Escherichia coli Involved in Efflux of Metabolites of the Cysteine Pathway, Mol. Microbiol., 2000, vol. 36, no. 5, pp. 1101–1112.

    Article  PubMed  Google Scholar 

  7. Franke, I., Resch, A., Daßler, T., et al., YfiK from Escherichia coli Promotes Export of o-Acetylserine and Cysteine, J. Bacteriol., 2003, vol. 185, no. 4, pp. 1161–1166.

    Article  PubMed  CAS  Google Scholar 

  8. Nandieni, R. and Gowrishankar, J., Evidence for an Arginine Exporter Encoded by yggA (argO) That Is Regulated by the LysR-Type Transcriptional Regulator ArgP in Escherichia coli, J. Bacteriol., 2004, vol. 186, no. 11, pp. 3539–3546.

    Article  Google Scholar 

  9. Brinkman, A., Ettema, T., de Vos, W., and van der Oost, J., The Lrp Family of Transcriptional Regulators, Mol. Microbiol., vol. 48, no. 2, pp. 287–294.

  10. DeLisa, M.P., Wu, C-F., Wang, L., et al., DNA Microarray-Based Identification of Genes Controlled by Autoinducer 2-Stimulated Quorum Sensing in Escherichia coli, J. Bacteriol., 2001, vol. 183, no. 18, pp. 5239–5247.

    Article  PubMed  CAS  Google Scholar 

  11. Doroshenko, V., Airich, L., Vitushkina, M., et al., YddG from Escherichia coli Promotes Export of Aromatic Amino Acids, FEMS Microbiol. Lett., 2007, vol. 275, no. 2, pp. 312–318.

    Article  PubMed  CAS  Google Scholar 

  12. Pittard, J., Camakaris, H., and Yang, J., The TyrR Regulon, Mol. Microbiol., 2005, vol. 55, no. 1, pp. 16–26.

    Article  PubMed  CAS  Google Scholar 

  13. Jensen, K.F., The Escherichia coli K-12 “Wild Types” W3110 and MG1655 Have an rph Frameshift Mutation That Leads to Pyrimidine Starvation due to Low pyrE Expression Levels, J. Bacteriol., 1993, vol. 175, pp. 3401–3407.

    PubMed  CAS  Google Scholar 

  14. Sambrook, J. and Russell, D.W., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Lab., 2001, 3rd ed.

    Google Scholar 

  15. Datsenko, K.A. and Wanner, B.L., One-Step Inactivation of Chromosomal Genes in Escherichia coli K-12 Using PCR Products, Proc. Natl. Acad. Sci. USA, 2000, vol. 97, pp. 6640–6645.

    Article  PubMed  CAS  Google Scholar 

  16. Miller, J.H., Experiments in Molecular Genetics, Cold Spring Harbor: Cold Spring Harbor Laboratories, 1972.

    Google Scholar 

  17. Link, A.J., Dereth, P., and Church, G.M., Methods for Generating Precise Deletions and Insertions in the Genome of Wild-Type Escherichia coli: Application to Open Reading Frame Characterization, J. Bacteriol., 1997, vol. 179, no. 30, pp. 6228–6237.

    PubMed  CAS  Google Scholar 

  18. Ovcharova, I.V., Eremina, S.Yu., and Mironov, A.S., Determination of Functional Role of Nucleotide Composition in the Transcription Start Region of the Escherichia coli udp Gene, Russ. J. Genet., 2003, vol. 39, no. 3, pp. 326–334.

    Article  CAS  Google Scholar 

  19. Typas, A. and Hengge, R., Role of the Spacer between the −35 and −10 Regions in σS Promoter Selectivity in Escherichia coli, Mol. Microbiol., 2006, vol. 59, no. 3, pp. 1037–1051.

    Article  PubMed  CAS  Google Scholar 

  20. Xiong, X.F. and Reznikoff, W.S., Transcriptional Slippage during the Transcription Initiation Process at a Mutant lac Promoter in vivo, J. Mol. Biol., 1993, vol. 231, no. 3, pp. 569–580.

    Article  PubMed  CAS  Google Scholar 

  21. Lewin, B., Genes VIII, Upper Saddle River: Prentice Hall, 2004.

    Google Scholar 

  22. Yang, J., Yoshito, O., Camakaris, H., et al., folA, a New Member of the TyrR Regulon in Escherichia coli K-12, J. Bacteriol., 2007, vol. 189, no. 16, pp. 6080–6084.

    Article  PubMed  CAS  Google Scholar 

  23. Polen, T., Krämer, M., Bongaerts, J., et al., The Global Gene Expression Response of Escherichia coli to L-Phenylalanine, J. Biotech., 2005, vol. 115, pp. 221–237.

    Article  CAS  Google Scholar 

  24. Csonka, L.N and Epstein, W, Osmoregulation, Escherichia coli and Salmonella: Cellular and Molecular Biology, Neidhardt, F.C., Curtiss III, R., Ingraham, J.L., et al., Eds., Washington DC: ASM Press, 1996, pp. 1210–1224.

    Google Scholar 

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Authors and Affiliations

  1. Ajinomoto-Genetika Research Institute, Moscow, 113545, Russia

    I. S. Tsyrenzhapova, V. G. Doroshenko, L. G. Airich & S. V. Mashko

  2. State Research Institute of Genetics and Selection of Industrial Microorganisms, Moscow, 113545, Russia

    A. S. Mironov

Authors
  1. I. S. Tsyrenzhapova
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  2. V. G. Doroshenko
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  3. L. G. Airich
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  4. A. S. Mironov
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  5. S. V. Mashko
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Correspondence to I. S. Tsyrenzhapova.

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Original Russian Text © I.S. Tsyrenzhapova, V.G. Doroshenko, L.G. Airich, A.S. Mironov, S.V. Mashko, 2009, published in Genetika, 2009, Vol. 45, No. 5, pp. 601–609.

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Tsyrenzhapova, I.S., Doroshenko, V.G., Airich, L.G. et al. Gene yddG of Escherichia coli encoding the putative exporter of aromatic amino acids: Constitutive transcription and dependence of the expression on the cell growth rate. Russ J Genet 45, 525–532 (2009). https://doi.org/10.1134/S1022795409050032

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  • DOI: https://doi.org/10.1134/S1022795409050032

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