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Induction, regeneration, and biolistic sensitivities of different genotypes of common wheat (Triticum aestivum L.)

  • Plant Genetics
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Abstract

The induction, regeneration, and biolistic sensitivities of different genotypes of common wheat (Triticum aestivum L.) have been determined in order to develop an efficient system for transformation of Russian cultivars of spring wheat. Short-term (two days) cold treatment (4°C) has been demonstrated to distinctly increase the frequency of morphogenetic callus induction. The optimal phytohormonal composition of the nutrient medium ensuring an in vitro regeneration rate of the common wheat cultivar Lada as high as 90% has been determined. The optimal temporal parameters of genetic transformation of wheat plants (10–14 days of culturing after initiation of a morphogenetic callus) have been determined for two transformation methods: biolistic without precipitated DNA and transformation with the plasmid psGFP-BAR. Analysis of the transient expression of the gfp gene has confirmed that 14 days of culturing is the optimal duration.

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, Moscow, 119991, Russia

    V. S. Fadeev & H. R. Shimshilashvili

  2. Center of Bioengineering, Russian Academy of Sciences, Moscow, 119312, Russia

    V. S. Fadeev & A. K. Gaponenko

  3. Kol’tsov Institute of Developmental Biology, Russian Academy of Sciences, Moscow, 117808, Russia

    A. K. Gaponenko

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  1. V. S. Fadeev
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  2. H. R. Shimshilashvili
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  3. A. K. Gaponenko
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Correspondence to V. S. Fadeev.

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Original Russian Text © V.S. Fadeev, H.R. Shimshilashvili, A.K. Gaponenko, 2008, published in Genetika, 2008, Vol. 44, No. 9, pp. 1257–1267.

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Fadeev, V.S., Shimshilashvili, H.R. & Gaponenko, A.K. Induction, regeneration, and biolistic sensitivities of different genotypes of common wheat (Triticum aestivum L.). Russ J Genet 44, 1096–1104 (2008). https://doi.org/10.1134/S1022795408090135

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  • DOI: https://doi.org/10.1134/S1022795408090135

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