Abstract
This paper reports for the first time about the detection and identification of ginsenoside malonyl-Rg1 (the rare 20(S)-protopanaxatriol-type ginsenoside) in the biomass of plant cell suspension culture of Japanese ginseng (Panax japonicus C.A. Mey. var. repens). Ginsenosides were analyzed by means of high-performance liquid chromatography/electrospray ionization mass spectrometry (HPLC-ESI-MS) in positive-ion mode. Malonyl-Rg1 was identified as a result of interpretation of MS spectra obtained upon fragmentation of protonated molecular ion ([M + H]+) of this compound in an ionization source. Chromatographic analysis and MS spectra showed that the cells of P. japonicus var. repens cultivated in vitro contain several isomers of malonyl-Rg1. Thus, we ascertained for the first time that, in addition to malonyl ginsenosides of 20(S)-protopanaxadiol group, the plant cell culture of ginseng P. japonicus var. repens can accumulate glycosides of 20(S)-protopanaxatriol group acylated with a malonic acid residue. The obtained results showed that, in the cells of ginseng cultivated in vitro for a long time (for 10 years and more), the assortment of secondary metabolites (ginsenosides) may be as wide as in intact plants.
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Original Russian Text © D.V. Kochkin, B.A. Galishev, E.S. Glagoleva, M.V. Titova, A.M. Nosov, 2017, published in Fiziologiya Rastenii, 2017, Vol. 64, No. 5, pp. 337–345.
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Kochkin, D.V., Galishev, B.A., Glagoleva, E.S. et al. Rare triterpene glycoside of ginseng (ginsenoside malonyl-Rg1) detected in plant cell suspension culture of Panax japonicus var. repens . Russ J Plant Physiol 64, 649–656 (2017). https://doi.org/10.1134/S102144371705003X
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DOI: https://doi.org/10.1134/S102144371705003X