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Different responses of two ecotypes of C3–C4 xero-halophyte Bassia sedoides to osmotic and ionic factors of salt stress

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Abstract

The effects of low and moderate salinity (100 and 200 mM NaCl, respectively) and iso-osmotic stress generated by polyethylene glycol PEG (1) (–0.3 MPa) and PEG (2) (–0.6 MPa) on maximum quantum yield of photosystem II (PSII), growth, photosynthesis, transpiration, dark respiration, water use efficiency (WUE), water content, chlorophyll, proline, Na+ and K+ concentrations were investigated in shoots of two ecotypes С3–С4 xero-halophyte Bassia sedoides (Pall.) Aschers. Plants were grown from seeds of two Southern Urals populations (Makan and Podolsk) differing in their bioproductivity. Aboveground biomass of the Makan plants was approximately 10-fold higher than that of the Podolsk ecotype. The plants of both ecotypes were sensitive to water deficit. They showed similar decrease in biomass, water content, net photosynthesis and transpiration intensity under both low and moderate osmotic stress (PEG). However, the content of сhlorophyll and free proline in shoots of the Podolsk plants increased under moderate osmotic stress (PEG(2)). Under salinity the differences between transpiration, F v/F m, WUE, water content, chlorophyll and proline concentrations in shoots of two ecotypes were no found. But, the Podolsk plants showed decrease in the growth parameters (1.5-fold), increase in the dark respiration intensity (2-fold) and the Na+/K+ ratio (1.2-fold) under moderate salinity (200 mM NaCl). Thus, the reduction of bioproductivity of the Podolsk ecotype under salinity was the result of ionic rather than osmotic factor of salinity. In the Podolsk plants the additional transpiration costs and consumption of assimilates (correspondingly) increased with the toxic sodium ion accumulation under salinity. This led to decrease in the growth parameters. Thus, two B. sedoides ecotypes have different adaptive strategies of tolerance to the ionic factor of salt stress at the level of the physiological processes associated with the dark CO2 gas exchange. Moreover, in less tolerant and productive Podolsk ecotype the increase in proline content in shoots characterized comparatively low adaptation to osmotic factor, and the increase in dark respiration and the Na+/K+ ratio pointed to relatively low resistance to ion factor of salinity as compared with the Makan ecotype.

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Abbreviations

F v/F m :

maximum quantum yield of PSII

F 0 :

minimal fluorescence from dark-adapted shoots

F m :

maximal fluorescence from dark-adapted shoots

PSI and PSII:

photosystem I and II.

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Authors and Affiliations

  1. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, ul. Botanicheskaya 35, Moscow, 127276, Russia

    Z. F. Rakhmankulova, E. V. Shuyskaya & P. Yu. Voronin

  2. Institute of Regional Researches, Academy of Sciences of the Republic Bashkortostan, ul. K. Tsetkin 2, Sibai, Bashkortostan, 453830, Russia

    Ya. T. Suyundukov

  3. Bashkir State University, ul. Zaki Validi 32, Ufa, Bashkortostan, 450074, Russia

    I. Yu. Usmanov

Authors
  1. Z. F. Rakhmankulova
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  2. E. V. Shuyskaya
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  3. Ya. T. Suyundukov
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  4. I. Yu. Usmanov
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  5. P. Yu. Voronin
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Correspondence to Z. F. Rakhmankulova.

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Published in Russian in Fiziologiya Rastenii, 2016, Vol. 63, No. 3, pp. 372–381.

The article was translated by the authors.

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Rakhmankulova, Z.F., Shuyskaya, E.V., Suyundukov, Y.T. et al. Different responses of two ecotypes of C3–C4 xero-halophyte Bassia sedoides to osmotic and ionic factors of salt stress. Russ J Plant Physiol 63, 349–357 (2016). https://doi.org/10.1134/S1021443716030122

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  • DOI: https://doi.org/10.1134/S1021443716030122

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