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Proteomic analysis of seed development in Chinese fir (Cunninghamia lanceolata)

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Abstract

Seed development is a complex process governed by highly coordinated changes in the expression of a large protein set. DIGE (Difference Gel Electrophoresis)-based proteomics was used to study developing Chinese fir seeds. 153 spots were obtained by using the analysis of DeCyder software (v. 6.5). Cluster analysis showed that they could be joined into three main groups. Eleven spots, more actively expressed at early cotyledonary stage of developing seeds, were identified by LC/MS/MS (tandem MS). Ten spots were identified by searching NCBInr or EST databases. They included two legumin-like storage proteins, LEA protein, small heat-shock protein, PR10-1.13, a protein similar to eukaryotic translation initiation factor, a protein similar to maternal effect embryo arrest 51, ORF115, a protein similar to monodehydroascorbate reductase, and unknown proteins. The potential function of these proteins during the precotyledonary stage of seed development was discussed.

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Abbreviations

2-DE:

two-dimensional gel electrophoresis

CHAPS:

3-[(3-cholamidopropyl)dimethylammonio]propanesulfonate

DIGE:

difference gel electrophoresis

LCMS/MS:

liquid chromatography mass spectrometry/mass spectrometry

IAA:

iodoacetamide

IEF:

isoelectric focusing

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Correspondence to Sen Shi Ji.

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Published in Russian in Fiziologiya Rastenii, 2010, Vol. 57, No.4, pp. 601–608.

This text was submitted by the authors in English.

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Zhen, Y., Ji, S.S., Zhao, Z.Z. et al. Proteomic analysis of seed development in Chinese fir (Cunninghamia lanceolata). Russ J Plant Physiol 57, 560–567 (2010). https://doi.org/10.1134/S1021443710040151

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