Abstract
Seed development is a complex process governed by highly coordinated changes in the expression of a large protein set. DIGE (Difference Gel Electrophoresis)-based proteomics was used to study developing Chinese fir seeds. 153 spots were obtained by using the analysis of DeCyder software (v. 6.5). Cluster analysis showed that they could be joined into three main groups. Eleven spots, more actively expressed at early cotyledonary stage of developing seeds, were identified by LC/MS/MS (tandem MS). Ten spots were identified by searching NCBInr or EST databases. They included two legumin-like storage proteins, LEA protein, small heat-shock protein, PR10-1.13, a protein similar to eukaryotic translation initiation factor, a protein similar to maternal effect embryo arrest 51, ORF115, a protein similar to monodehydroascorbate reductase, and unknown proteins. The potential function of these proteins during the precotyledonary stage of seed development was discussed.
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Abbreviations
- 2-DE:
-
two-dimensional gel electrophoresis
- CHAPS:
-
3-[(3-cholamidopropyl)dimethylammonio]propanesulfonate
- DIGE:
-
difference gel electrophoresis
- LCMS/MS:
-
liquid chromatography mass spectrometry/mass spectrometry
- IAA:
-
iodoacetamide
- IEF:
-
isoelectric focusing
References
Huang, Z., Xu, Z., Boyd, S., and Willams, D., Chemical Composition of Decomposing Stumps in Successive Rotation of Chinese Fir (Cunninghamia lanceolata (Lamb.) Hook.) Plantations, Chinese Sci. Bull., 2005, vol. 50, pp. 2581–2586.
Goldberg, R.B., de Paiva, G., and Yadegari, R., Plant Embryogenesis: Zygote to Seed, Science, 1994, vol. 266, pp. 605–614.
Dodeman, V.L., Ducreux, G., and Kreis, M., Zygotic Embryogenesis versus Somatic Embryogenesis, J. Exp. Bot., 1997, vol. 48, pp. 1493–1509.
Dos Santos, A.L., Wiethölter, N., El Gueddari, N.E., and Moerschbacher, B.M., Protein Expression during Seed Development in Araucaria angustifolia: Transient Accumulation of Class IV Chitinases and Arabinogalactan Proteins, Physiol. Plant., 2006, vol. 127, pp. 138–148.
Sallandrouze, A., Faurobert, M., and El Maâtaoui, M., Characterization of the Developmental Stages of Cypress Zygotic Embryos by Two-Dimensional Electrophoresis and by Cytochemistry, Physiol. Plant., 2002, vol. 114, pp. 608–618.
Lippert, D., Zhuang, J., Ralph, S., Ellis, D.E., and Gilbert, M., Proteome Analysis of Early Somatic Embryogenesis in Picea glauca, Proteomics, 2005, vol. 5, pp. 461–473.
Klimaszewska, K., Morencya, F., Jones-Overtona, C., and Cookeb, J., Accumulation Pattern and Identification of Seed Storage Proteins in Zygotic Embryos of Pinus strobus and in Somatic Embryos from Different Maturation Treatments, Physiol. Plant., 2004, vol. 121, pp. 682–690.
Jiang, S., Anatomical Observation of Flowering and Seed Development of Cunninghamia lanceolata, J. Nanjing Forestry University (Natural Sciences Edition), 1980, vol. 1, pp. 109–115.
Jiang, S. and Ye, J.G., Research on Embryonic Development of Cunninghamia lanceolata and Cryptomeria janponica, J. Nanjing Forestry University (Natural Sciences Edition), 1984, vol. 1, pp. 124–130.
Cairney, J. and Pullman, G.S., The Cellular and Molecular Biology of Conifer Embryogenesis, New Phytol., 2007, vol. 176, pp. 511–536.
Jain, S.M., Gupta, P.K., and Newton, R.J., Somatic Embryogenesis in Woody Plants, Dordrecht: Kluwer, 2000.
Von Arnold, S., Sabala, I., Bozhkov, P., Dyachok, J., and Filonova, L., Developmental Pathways of Somatic Embryogenesis, Plant Cell, Tissue Organ Cult., 2000, vol. 69, pp. 233–249.
West, M.A.L. and Harada, J.J., Embryogenesis in Higher Plants: An Overview, Plant Cell, 1993, vol. 5, pp. 1361–1369.
Cuming, A.C., LEA Proteins, Dordrecht: Kluwer, 1999.
Stasolla, C. and Yeung, E.C., Ascorbic Acid Metabolism during White Spruce Somatic Embryo Maturation and Germination, Physiol. Plant., 2001, vol. 111, pp. 196–205.
Kawahara, R., Sumabori, S., Fukuda, H., and Komamine, A., A Gene Expressed Preferentially in the Globular Stage of Somatic Embryogenesis Encodes Elongation-Factor 1 in Carrot, Eur. J. Biochem., 1992, vol. 209, pp. 157–162.
Huh, J.H., Bauer, M.J., Hsieh, T.F., and Fischer, R., Endosperm Gene Imprinting and Seed Development, Curr. Opin. Genet. Dev., 2007, vol. 17, pp. 480–485.
Grimanelli, D.L., Perotti, E., Ramirez, J., and Leblanca, O., Timing of the Maternal-to-Zygotic Transition during Early Seed Development in Maize, Plant Cell, 2005, vol. 17, pp. 1061–1072.
Graves, P.R. and Haystead, T.A.J., Molecular Biologist’s Guide to Proteomics, Microbiol. Mol. Biol. Res., 2002, vol. 66, pp. 39–63.
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Published in Russian in Fiziologiya Rastenii, 2010, Vol. 57, No.4, pp. 601–608.
This text was submitted by the authors in English.
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Zhen, Y., Ji, S.S., Zhao, Z.Z. et al. Proteomic analysis of seed development in Chinese fir (Cunninghamia lanceolata). Russ J Plant Physiol 57, 560–567 (2010). https://doi.org/10.1134/S1021443710040151
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DOI: https://doi.org/10.1134/S1021443710040151