Abstract
The mutant line of Arabidopsis thaliana obtained by insertion mutagenesis of fro gene (at5g67590) encoding the 18-kD Fe-S subunit of mitochondrial respiratory chain complex I was characterized. The homozygous plants carrying the insert were selected by PCR genotyping. The absence in these plants of transcripts corresponding in size to the fro gene was confirmed by gene expression analysis. The homozygous plants obtained were characterized by several phenotypic features, such as late seed germination, retarded growth and development, the increased number and deep-green color of leaves. At variance with earlier data obtained for splicing-defective mutants of the Arabidopsis fro gene, the plants obtained in this study did not exhibit a lower resistance to abiotic stresses. Despite complete inactivation of the fro gene, the mutant plants were similar to the wild-type plants in terms of biomass growth and productivity. It is concluded that regulatory systems for gene expression in mutant Arabidopsis plants with impaired respiratory complex I are able to largely compensate for the respiratory deficiency at the phenotypic level.
Similar content being viewed by others
Abbreviations
- CMS:
-
cytoplasmic male sterility
- ETC:
-
electron transport chain
- PCR:
-
polymerase chain reaction
- RT-PCR:
-
reverse transcription PCR
References
Newton, K.J., Gabay-Laughnan, S., and de Paepe, R., Mitochondrial Mutations in Plants, Advances in Photosynthesis and Respiration, vol. 17, Plant Mitochondria: From Genome to Function, Day, D.A., Millar, H.A., and Whelan, J., Eds., Boston: Kluwer, 2004, pp. 121–142.
Marienfeld, J.R. and Newton, K.J., The Maize NCS2 Abnormal Growth Mutant Has a Chimeric nad4-nad7 Mitochondrial Gene and Is Associated with Reduced Complex I Function, Genetics, 1994, vol. 138, pp. 855–863.
Gutierres, S., Sabar, M., Lelandais, C., Chetrit, P., Diolez, P., Degand, H., Boutry, M., Vedel, F., de Kouchkovsky, Y., and de Paepe, R., Lack of Mitochondrial and Nuclear-Encoded Subunits of Complex I and Alteration of the Respiratory Chain in Nicotiana sylvestris Mitochondrial Deletion Mutants, Proc. Natl. Acad. Sci. USA, 1997, vol. 94, pp. 3436–3441.
Brangeon, J., Sabar, M., Gutierres, S., Combettes, B., Bove, J., Gendy, C., Chetrit, P., des Francs-Small, C.C., Pla, M., Vedel, F., and de Paepe, R., Defective Splicing of the First nad4 Intron Is Associated with Lack of Several Complex I Subunits in the Nicotiana sylvestris NMS1 Nuclear Mutant, Plant J., 2000, vol. 21, pp. 269–280.
Priault, P., Fresneau, C., Noctor, G., de Paepe, R., Cornic, G., and Streb, P., The Mitochondrial CMSII Mutation of Nicotiana sylvestris Impairs Adjustment of Photosynthetic Carbon Assimilation to Higher Growth Irradiance, J. Exp. Bot., 2006, vol. 57, pp. 2075–2085.
Vidal, G., Ribas-Carbo, M., Garmier, M., Dubertret, G., Rasmusson, A.G., Mathieu, C., Foyer, C.H., and de Paepe, R., Lack of Respiratory Chain Complex I Impairs Alternative Oxidase Engagement and Modulates Redox Signaling during Elicitor-Induced Cell Death in Tobacco, Plant Cell, 2007, vol. 19, pp. 640–655.
Dutilleul, C., Garmier, M., Noctor, G., Mathieu, C., Chetrit, P., Foyer, C.H., and de Paepe, R., Leaf Mitochondria Modulate Whole Cell Redox Homeostasis, Set Antioxidant Capacity, and Determine Stress Resistance, Plant Cell, 2003, vol. 15, pp. 1212–1226.
De Longevialle, A.F., Meyer, E.H., Andres, C., Taylor, N.L., Lurin, C., Millar, A.H., and Small, I.D., The Pentatricopeptide Repeat Gene OTP43 Is Required for Trans-Splicing of the Mitochondrial nad1 Intron 1 in Arabidopsis thaliana, Plant Cell, 2007, vol. 19, pp. 3256–3265.
Lee, B.H., Lee, H., Xiong, L., and Zhu, J.K., A Mitochondrial Complex I Defect Impairs Cold-Regulated Nuclear Gene Expression, Plant Cell, 2002, vol. 14, pp. 1235–1251.
Sessions, A., Burke, E., Presting, G., Aux, G., McElver, J., Patton, D., Dietrich, B., Ho, P., Bacwaden, J., Ko, C., Clarke, J.D., Cotton, D., Bullis, D., Snell, J., Miguel, T., Hutchison, D., Kimmerly, B., Mitzel, T., Katagiri, F., Glazebrook, J., Law, M., and Goff, S.A., A High-Throughput Arabidopsis Reverse Genetics System, Plant Cell, 2002, vol. 14, pp. 2985–2994.
Murashige, T. and Skoog, F., Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Culture, Physiol. Plant., 1962, vol. 15, pp. 473–497.
Doyle, J.J. and Doyle, J.L., A Rapid Isolation Program for Small Quantities of Fresh Leaf Tissue, Phytochem. Bull., 1987, vol. 19, pp. 11–15.
Garnik, E.Yu., Tarasenko, V.I., Kobzev, V.F., and Konstantinov, Yu.M., Differential Expression of Maize Mitochondrial Genes as Dependent on Mitochondria Redox State, Russ. J. Plant Physiol., 2006, vol. 53, pp. 463–468.
Katyshev, A.I., Konstantinov, Yu.M., and Kobzev, V.F., Characterization of Mn- and Cu/Zn-Containing Superoxide Dismutase Gene Transcripts in Larix gmelinii, Mol. Biol., 2006, vol. 40, pp. 327–329.
Dufour, E., Boulay, J., Rincheval, V., and Sainsard-Chanet, A., A Causal Link between Respiration and Senescence in Podospora anserine, Proc. Natl. Acad. Sci. USA, 2000, vol. 97, pp. 4138–4143.
Rea, S.L., Metabolism in the Caenorhabditis elegans Mit Mutants, Exp. Gerontol., 2005, vol. 40, pp. 841–849.
Lorin, S., Dufour, E., and Sainsard-Chanet, A., Mitochondrial Metabolism and Aging in the Filamentous Fungus Podospora anserine, Biochim. Biophys. Acta, 2006, vol. 1757, pp. 604–610.
Heazlewood, J.L., Howell, K.A., and Millar, A.H., Mitochondrial Complex I from Arabidopsis and Rice: Orthologs of Mammalian and Fungal Components Coupled with Plant-Specific Subunits, Biochim. Biophys. Acta, 2003, vol. 1604, pp. 159–169.
Remacle, C., Barbieri, M.R., Cardol, P., and Hamel, P.P., Eukaryotic Complex I: Functional Diversity and Experimental Systems to Unravel the Assembly Process, Mol. Genet. Genom., 2008, vol. 280, pp. 93–110.
Lister, R., Chew, O., Lee, M., Heazlewood, J.L., Clifton, R., and Parker, K.L., A Transcriptomic and Proteomic Characterization of the Arabidopsis Mitochondrial Protein Import Apparatus and Its Response to Mitochondrial Dysfunction, Plant Physiol., 2004, vol. 134, pp. 777–789.
Clifton, R., Lister, R., Parker, K.L., Sappl, P.G., Elhafez, D., Millar, A.H., Day, D.A., and Whelan, J., Stress-Induced Co-Expression of Alternative Respiratory Chain Components in Arabidopsis thaliana, Plant Mol. Biol., 2005, vol. 58, pp. 193–212.
Karpova, O.V., Kuzmin, E.V., Elthon, T.E., and Newton, K.J., Differential Expression of Alternative Oxidase Genes in Maize Mitochondrial Mutants, Plant Cell, 2002, vol. 14, pp. 3271–3284.
Tarasenko, V.I., Garnik, E.Yu., Shmakov, V.N., and Konstantinov, Yu.M., Induction of Arabidopsis gdh2 Gene Expression during Changes in Redox State of the Mitochondrial Respiration Chain, Biochimiya, 2009, vol. 74, pp. 47–53.
Author information
Authors and Affiliations
Corresponding author
Additional information
Original Russian Text © V.I. Tarasenko, E.Yu. Garnik, Yu.M. Konstantinov, 2010, published in Fiziologiya Rastenii, 2010, Vol. 57, No.3, pp. 415–424.
Rights and permissions
About this article
Cite this article
Tarasenko, V.I., Garnik, E.Y. & Konstantinov, Y.M. Characterization of Arabidopsis mutant with inactivated gene coding for Fe-S subunit of mitochondrial respiratory chain complex I. Russ J Plant Physiol 57, 392–400 (2010). https://doi.org/10.1134/S1021443710030118
Received:
Published:
Issue Date:
DOI: https://doi.org/10.1134/S1021443710030118