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Effect of kinetin on early stages of chlorophyll biosynthesis in streptomycin-treated barley seedlings

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Abstract

Treatment of barley seeds (Hordeum vulgare L.) with streptomycin, an inhibitor of plastid protein synthesis, resulted in growth of the albino phenotype seedlings with ribosome-deficient undifferentiated plastids and chlorophyll (Chl) level as low as 0.1% of that in control plant leaves. A major effect of the antibiotic was almost complete suppression of the ability of plants to synthesize 5-aminolevulinic acid (ALA) intended for Chl biosynthesis. The activity of synthesis of ALA intended for heme porphyrin biosynthesis in etiolated and greening seedlings and in light-grown albinophenotype plants was insensitive to light and cytokinins. In the upper parts of leaves of streptomycin-treated plants, exhibiting 60% Chl deficit, the cells with three types of chloroplasts could be observed: normally developed chloroplasts, chloroplasts composed of single thylakoids and grana, and completely undifferentiated plastids. In this Chl-deficient tissue, ALA synthesis was found to be stimulated by kinetin but much less than in leaves of the control plants. The endogenous cytokinin content in etiolated and greening seedlings treated with streptomycin was almost the same as it was in untreated control seedlings. The cytokinin level in the white tissue of plants grown in the light was on average twice as high as that in green leaves of the control plants. The capability of kinetin to stimulate the synthesis of ALA used for Chl biosynthesis was found to correlate with the Chl content and organization of the chloroplast internal structure. This correlation confirms the hypothesis that the normally developed internal structure of plastids is essential for the adequate phytohormone response in plants.

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Abbreviations

ALA:

5-aminolevulinic acid

Chl:

chlorophyll

LA:

levulinic acid

References

  1. Khokhlova, V.A., Sveshnikova, I.N., and Kulaeva, O.N., Effects of Phytohormones on Formation of Chloroplast Structure in Isolated Pumpkin Seedlings, Tsitologiya, 1971, vol. 13, pp. 1074–1078.

    CAS  Google Scholar 

  2. Chory, J., Reinecke, D., Sim, S., Washburn, T., and Brenner, M., A Role for Cytokinins in De-Etiolation in Arabidopsis (det Mutants Have an Altered Response to Cytokinins), Plant Physiol., 1994, vol. 104, pp. 339–347.

    PubMed  CAS  Google Scholar 

  3. Serve, B.T., Axelos, M., and Peaud-Lenoel, C., Cytokinins Modulate the Expression of Genes Encoding the Protein of the Light-Harvesting Chlorophyll a/b Complex, Plant. Mol. Biol., 1985, vol. 5, pp. 155–163.

    Article  Google Scholar 

  4. Flores, S. and Tobin, E.M., Benzyladenine Modulation of the Expression of Two Genes for Nuclear-Encoded Chloroplast Proteins in Lemna gibba: Apparent Post-Transcriptional Regulation, Planta, 1986, vol. 168, pp. 340–349.

    Article  CAS  Google Scholar 

  5. Ohya, T. and Suzuki, H., The Effect of Benzyladenine on the Accumulation of Messenger RNAs That Encode the Large and Small Subunits of Rubisco and Light Harvesting Chlorophyll a/b-Protein in Excised Cucumber Cotyledons, Plant Cell Physiol., 1991, vol. 32, pp. 577–580.

    CAS  Google Scholar 

  6. Kusnetsov, V.V., Oelmüller, R., Sarwat, M.I., Porfirova, S.A., Cherepneva, G.N., Herrmann, R.G., and Kulaeva, O.N., Cytokinin, Abscisic Acid and Light Affect Accumulation of Chloroplast Proteins in Lupinus luteus Cotyledons without Notable Effect on Steady-State mRNAs Levels, Planta, 1994, vol. 194, pp. 318–327.

    Article  CAS  Google Scholar 

  7. Sigiura, M., Promotion of Chlorophyll Synthesis by Kinetin, Bot. Mag. (Tokyo), 1963, vol. 76, pp. 309–310.

    Google Scholar 

  8. Averina, N.G. and Shlyk, A.A., Effect of Kinetin on Accumulation and Activity of Protochlorophyllide in Etiolated and Post-Etiolated Barley Leaves, Sov. Plant Physiol., 1972, vol. 19, pp. 487–496.

    CAS  Google Scholar 

  9. Fletcher, R., Teo, S., and Alt, A., Stimulation of Chlorophyll Synthesis in Cucumber Cotyledons by Benzyladenine, Can. J. Bot., 1973, vol. 51, pp. 937–939.

    CAS  Google Scholar 

  10. Kusnetsov, V.V., Herrmann, R.G., Kulaeva, O.N., and Oelmüller, R., Cytokinin Stimulates and Abscisic Acid Inhibits Greening of Etiolated Lupinus luteus Cotyledons by Affecting the Expression of the Light-Sensitive Protochlorophyllide Oxidoreductase, Mol. Gen. Genet., 1998, vol. 259, pp. 21–28.

    PubMed  CAS  Google Scholar 

  11. Masuda, T., Ohta, H., Shioi, Y., Tsuji, H., and Takamiya, K., Stimulation of Glutamyl-tRNA Reductase Activity by Benzyladenine in Greening Cucumber Cotyledons, Plant Cell Physiol., 1995, vol. 36, pp. 1237–1243.

    CAS  Google Scholar 

  12. Vershilovskaya, I.V., Yaronskaya, E.B., Vezitskii, A.Yu., and Averina, N.G., Investigation of Cytokinin Action Mechanisms on the System of Chlorophyll Biosynthesis, Izv. Nat. Akad. Nauk Belarusi, Ser. Biol. nauk, 2004, no. 1, pp. 80–84.

  13. Kulaeva, O.N., Burkhanova, E.A., Karavaiko, N.N., Selivankina, S.Y., Porfirova, S.A., Maslova, G.G., Zemlyachenko, Y.V., and Börner, T., Chloroplasts Affect the Leaf Response to Cytokinin, J. Plant Physiol., 2002, vol. 159, pp. 1309–1316.

    Article  CAS  Google Scholar 

  14. Zubko, M.K. and Day, A., Stable Albinism Induced without Mutagenesis: A Model for Ribosome-Free Plastid Inheritance, Plant J., 1998, vol. 15, pp. 265–271.

    Article  PubMed  CAS  Google Scholar 

  15. Zubko, M.K. and Day, A., Differential Regulation of Genes Transcribed by Nucleus-Encoded Plastid RNA Polymerase, and DNA Amplification, within Ribosome-Deficient Plastids in Stable Phenocopies of Cereal Albino Mutants, Mol. Gen. Genomics, 2002, vol. 267, pp. 27–37.

    Article  CAS  Google Scholar 

  16. Demmig-Adams, B. and Adams, W.W., Chlorophyll and Carotenoid Composition in Leaves of Euonymus kiautschovicus Acclimated to Different Degrees of Light Stress in the Field, Aust. J. Plant Physiol., 1996, vol. 23, pp. 649–659.

    Article  CAS  Google Scholar 

  17. Miller, G.W., Denney, A., Wood, J.K., and Welkie, G.W., Light-Induced delta-Aminolevulinic Acid in Dark-Grown Barley Seedlings, Plant Cell Physiol., 1979, vol. 20, pp. 131–143.

    CAS  Google Scholar 

  18. Mauzerall, D. and Granick, S., The Occurrence and Determination of 5-Aminolevulinic Acid and Porphobilinogen in Urine, J. Biol. Chem., 1956, vol. 219, pp. 435–446.

    PubMed  CAS  Google Scholar 

  19. Lezhneva, L.A., Rassadina, V.V., Yaronskaya, E.B., Radyuk, M.S., Taran, S.F., and Averina, N.G., Activity of the Fe-Branch of Tetrapyrrole Biosynthesis in the Chlorophyll-Deficient Plastome Mutant of Sunflower (Helianthus annuus L.), Russ. J. Plant Physiol., 2001, vol. 48, pp. 57–61.

    Article  CAS  Google Scholar 

  20. Moskaleva, O.V. and Karavaiko, N.N., Dynamics of Endogenous Phytohormones during Development of Maize Seedlings, Sov. Plant Physiol., 1990, vol. 37, pp. 1113–1120.

    Google Scholar 

  21. Kudoyarova, G.R., Veselov, S.Yu., Karavaiko, N.N., Gyuli-zade, V.Z., Cheredova, E.P., Mustafina, A.R., Moshkov, I.E., and Kulaeva, O.N., Immunoenzymatic Test-System for Cytokinin Determination, Sov. Plant Physiol., 1990, vol. 37, pp. 193–199.

    CAS  Google Scholar 

  22. Beale, S.I. and Castelfranco, P.A., The Biosynthesis of δ-Aminolevulinic Acid in Higher Plants: I. Accumulation of δ-Aminolevulinic Acid in Greening Plant Tissues, Plant Physiol., 1974, vol. 53, pp. 291–296.

    Article  PubMed  CAS  Google Scholar 

  23. Yaronskaya, E., Ziemann, V., Walter, G., Averina, N., Börner, T., and Grimm, B., Metabolic Control of the Tetrapyrrole Biosynthetic Pathway for Porphyrin Distribution in the Barley Mutant albostrians, Plant J., 2003, vol. 35, pp. 512–522.

    Article  PubMed  CAS  Google Scholar 

  24. Papenbrock, J. and Grimm, B., Regulatory Network of Tetrapyrrole Biosynthesis — Studies for Intracellular Signaling Involved in Metabolic and Developmental Control of Plastids, Planta, 2001, vol. 213, pp. 667–681.

    Article  PubMed  CAS  Google Scholar 

  25. Surpin, M., Larkin, R.M., and Chory, J., Signal Transduction between the Chloroplast and the Nucleus, Plant Cell, 2002, vol. 14,Suppl., pp. S327–S338.

    PubMed  CAS  Google Scholar 

  26. Bougri, O. and Grimm, B., Members of a Low-Copy Number Gene Family Encoding Glutamyl-tRNA Reductase Are Differentially Expressed in Barley, Plant J., 1996, vol. 9, pp. 867–878.

    Article  PubMed  CAS  Google Scholar 

  27. Tanaka, R., Yoshida, K., Nakayashiki, T., Masuda, T., Tsuji, H., Inokuchi, H., and Tanaka, A., Differential Expression of Two hemA mRNAs Encoding Glutamyl-tRNA Reductase Proteins in Greening Cucumber Seedlings, Plant Physiol., 1996, vol. 110, pp. 1223–1230.

    Article  PubMed  CAS  Google Scholar 

  28. Tanaka, R., Yoshida, K., Nakayashiki, T., Tsuji, H., Inokuchi, H., Okada, K., and Tanaka, A., The Third Member of the hemA Gene Family Encoding Glutamyl-tRNA Reductase Is Primarily Expressed in Roots in Hordeum vulgare, Photosynth. Res., 1997, vol. 53, pp. 161–171.

    Article  CAS  Google Scholar 

  29. Yaronskaya, E.B., Vershilovskaya, I.V., and Averina, N.G., Effect of Kinetin on Activity of Heme Branch of Tetrapyrrole Biosynthesis in Hordeum vulgare L. Seedlings, Izv. Nat. Akad. Nauk Belarusi, Ser. Biol. nauk, 2004, no. 2, pp. 79–82.

  30. Kof, E.M., Kefeli, V.I., Kutachek, M., Eder, I., Chermak, V., and Gostimskii, S.A., The Effects of Mutation-Induced Chlorophyll Deficiency and Light on Phytohormones in Pea Plants, Russ. J. Plant Physiol., 1994, vol. 41, pp. 592–598.

    Google Scholar 

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Original Russian Text © E.B. Yaronskaya, E.R. Griskevich, N.L. Trukhanovets, N.G. Averina, 2007, published in Fiziologiya Rastenii, 2007, Vol. 54, No. 3, pp. 440–448.

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Yaronskaya, E.B., Gritskevich, E.R., Trukhanovets, N.L. et al. Effect of kinetin on early stages of chlorophyll biosynthesis in streptomycin-treated barley seedlings. Russ J Plant Physiol 54, 388–395 (2007). https://doi.org/10.1134/S1021443707030144

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  • DOI: https://doi.org/10.1134/S1021443707030144

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