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Morphology and Phytohormone Content in Transgenic Tobacco Plants Expressing Bacterial Thermostable Cellulase

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Abstract

Expression of the bacterial gene for thermostable β-1,4-glucanase (cellulase) from Clostridium thermocellum in transgenic tobacco plants was shown to produce significant changes in tobacco plant structure and activities. The transgenic plants differed in their growth rate and morphology, and their hormonal status was affected. Thus, the transgenic plants expressing the gene for thermostable bacterial cellulase are a convenient model to study the role of β-1,4-glucanases in plant physiological processes.

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REFERENCES

  1. Bayer, E.A., Shimon, L.J.W., Shoham, Y., and Lamed, R., Cellulosomes: Structure and Ultrastructure, J. Struct. Biol., 1998, vol. 124, pp. 221-234.

    PubMed  Google Scholar 

  2. Beguin, P., Molecular Biology of Cellulose Degradation, Annu. Rev. Microbiol., 1990, vol. 44, pp. 219-248.

    PubMed  Google Scholar 

  3. Del Campillo, E. and Bennett, A.B., Pedicel Break-strength and Cellulase Gene Expression during Tomato Flower Abscission, Plant Physiol., 1996, vol. 1113, pp. 813-820.

    Google Scholar 

  4. Del Campillo, E., Multiple Endo-1,4-β-D-Glucanase (Cellulase) Genes in Arabidopsis, Curr. Top. Dev. Biol., 1999, vol. 46, pp. 39-61.

    Google Scholar 

  5. Hobson, G.E., Cellulase Activity during the Maturation and Ripening of Tomato Fruit, J. Food Sci., 1968, vol. 33, pp. 588-592.

    Google Scholar 

  6. Brummell, D.A., Catala, C., Lashbrook, C.C., and Bennett, A.B., A Membrane-Anchored E-Type Endo-1,4-β-Glucanase Is Localized on Golgi and Plasma Membranes of Higher Plants, Proc. Natl. Acad. Sci. USA, 1997, vol. 94, pp. 4794-4799.

    PubMed  Google Scholar 

  7. Piruzian, E.S., Goldenkova, I.V., Musiichuk, K.A., Abdeev, R.M., Volkova, L.V., and Kobets, N.S., A New Reporter System for Studying Plant Gene Expression Based on Lichenase High Thermostability, Fiziol. Rast. (Moscow), 2000, vol. 47, pp. 382-389 (Russ. J. Plant Physiol., Engl. Transl.).

    Google Scholar 

  8. Movsesian, N.R., Alizade, Kh., Goldenkova, I.V., Musiichuk, K.A., Popov, Yu.G., and Piruzian, E.S., Transgenic Tobacco Plants Expressing Bacterial Genes of Thermostable Glucanase, Genetika, 2001, vol. 37, pp. 745-752.

    PubMed  Google Scholar 

  9. Gudliemli, G. and Beguin, P., Cellulase and Hemicellulase Genes of Clostridium thermocellum from Five Independent Collections Contain Few Overlaps and Are Widely Scattered across the Chromosome, FEMS Microbiol. Lett., 1998, vol. 161, pp. 209-215.

    PubMed  Google Scholar 

  10. Bayer, E.A., Shimon, L.J., Shoham, Y., and Lamed, R., Cellulosomes: Structure and Ultrastructure, J. Struct. Biol., 1998, vol. 124, pp. 221-234.

    PubMed  Google Scholar 

  11. Hall, J., Hazlewood, G.P., Barker, P.J., and Gilbert, H.J., Conserved Reiterated in Clostridium thermocellum Endoglucanase Are Not Essential for Catalytic Activity, Gene, 1988, vol. 69, pp. 29-38.

    PubMed  Google Scholar 

  12. Abdeev, R.M., Goldenkova, I.V., Musiichuk, K.A., and Piruzian, E.S., Expression of Thermostable Bacterial Cellulase in Transgenic Tobacco Plants, Genetika, 2003, vol. 39, pp. 376-382.

    PubMed  Google Scholar 

  13. Kudoyarova, G.R., Veselov, S.Yu., Karavaiko, N.N., Gyulizade, V.Z., Cheredova, E.P., Mustafina, A.R., Moshkov, I.E., and Kulaeva, O.N., Immunoenzyme Test-System for the Determination of Cytokinins, Fiziol. Rast. (Moscow), 1990, vol. 37, pp. 193-199 (Sov. Plant Physiol., Engl. Transl.).

    Google Scholar 

  14. Kholodar', V.A., Shevtsov, S.V., and Chekurov, V.M., Immunoenzyme Assay in the Study of Photoregulated Gibberellin Level in Wheat Etioplasts, Fiziol. Rast. (Moscow), 1995, vol. 42, pp. 647-651 (Russ. J. Plant Physiol., Engl. Transl.).

    Google Scholar 

  15. Abdeev, R.M., Goldenkova, I.V., Musiichuk, K.A., and Piruzian, E.S., Study of the Properties of Thermostable Cellulase CelE from Clostridium thermocellum for the Purpose of Its Expression in Plants, Biokhimiya, 2001, vol. 66, pp. 991-998.

    Google Scholar 

  16. Bowler, C. and Chua, N., Emerging Themes of Plant Signal Transduction, Plant Cell, 1994, vol. 6, pp. 1529-1541.

    Article  PubMed  Google Scholar 

  17. Davies, P.J., tiPlant Hormones: Physiology, Biochemistry, and Molecular Biology, Dordrecht: Kluwer, 1995, pp. 572-597.

  18. Matsumoto, T., Sakai, F., and Hayashi, T., A Xyloglucan-Specific Endo-1,4βGlucanase Isolated from Auxin-Treated Pea Stems, Plant Physiol., 1997, vol. 114, pp. 661-667.

    PubMed  Google Scholar 

  19. Polevoi, V.V., tiRol' auksina v sistemakh regulyatsii u rastenii, 44-e Timiryazevskoe chtenie (Role of Auxin in the Systems of Plant Regulation, the 44th Timiryazev Lecture), Leningrad: Nauka, 1986.

  20. Tret'yakov, N.N., tiFiziologiya i biokhimiya sel'skokhozyaistvennykh rastenii (Crop Physiology and Biochemistry), Moscow: Kolos, 1998.

  21. Hare, P.D. and van Staden, J., The Molecular Basis of Cytokinin Action, Plant Growth Regul., 1997, vol. 23, pp. 41-78.

    Google Scholar 

  22. Zabotina, O.A., Gur'yanov, O.P., Malikov, R.G., Ayupova, D.A., Beldman, G., Varagen, A.D.G., and Lozovaya, V.V., The Isolation and Biological Activity of Oligosaccharins from the Pea Shoots, Fiziol. Rast. (Moscow), 1995, vol. 42, pp. 416-422 (Russ. J. Plant Physiol., Engl. Transl.).

    Google Scholar 

  23. Albersheim, P. and Darvill, A.G., Oligosaccharins: Novel Molecules That Can Regulate Growth, Development, Reproduction, and Defense against Disease in Plants, Sci. Am., 1985, vol. 253, pp. 58-64.

    PubMed  Google Scholar 

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Authors and Affiliations

  1. Vavilov Institute of General Genetics, Russian Academy of Sciences, ul. Gubkina 3, Moscow, 117809, Russia

    R. M. Abdeev, K. A. Musiichuk, I. V. Goldenkova, D. V. Sotchenkov, G. R. Salekhi Dzhuzani & E. S. Piruzian

  2. Timiryazev Institute of Plant Physiology, Russian Academy of Sciences, Botanicheskaya ul. 35, Moscow, 127276, Russia

    A. K. Alyavina & N. V. Zagoskina

Authors
  1. R. M. Abdeev
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  2. K. A. Musiichuk
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  3. I. V. Goldenkova
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  4. D. V. Sotchenkov
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  5. G. R. Salekhi Dzhuzani
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  6. A. K. Alyavina
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  7. N. V. Zagoskina
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  8. E. S. Piruzian
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Abdeev, R.M., Musiichuk, K.A., Goldenkova, I.V. et al. Morphology and Phytohormone Content in Transgenic Tobacco Plants Expressing Bacterial Thermostable Cellulase. Russian Journal of Plant Physiology 51, 642–647 (2004). https://doi.org/10.1023/B:RUPP.0000040751.62093.43

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  • DOI: https://doi.org/10.1023/B:RUPP.0000040751.62093.43

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