Abstract
To determine the positional-species composition (PSC) of the sunflower oil triacylglycerols (TAGs), these TAGs were separated in a native state by preparative TLC on alumina and subjected to a partial chemical deacylation with the Grignard reagent under anhydrous conditions. The rac-1,2-diacylglycerols (DAGs) formed in this reaction were identified by comparing their TLC mobilities with the respective standard separated from the products of enzymatic hydrolysis of lecithin. Subsequently, the DAGs as their borate complexes were isolated from the reaction mixture by preparative TLC on silica gel. The quantitative fatty acid composition of both the DAGs and the initial TAGs was determined by capillary GLC. The PSC of TAGs calculated from the data obtained using a computer program was consistent with the data on sunflower TAG composition reported by other researchers using an independent method. Thus, the technique developed here makes it possible to obtain reliable data on the concentration of all TAG positional species in the plant tissue.
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REFERENCES
Vereshchagin, A.G., Biokhimiya triglitseridov (Biochemistry of Triglycerides), Moscow: Nauka, 1972.
Litchfield, C., Analysis of Triglycerides, New York: Academic, 1972.
Kuksis, A., Analysis of Positional Isomers of Glycerolipids by Non-Enzymatic Methods, Advances in Lipid Methodology vol. 3, Christie, W.W., Ed., Dundee: The Oily Press, 1996, pp. 1–36.
Kirchner, J.G., Thin-Layer Chromatography, New York: Intersci. Publ., 1967.
Bergel'son, L.D., Dyatlovitskaya, E.V., Molotkovsky, Yu.G., Batrakov, S.G., Barsukov, L.I., and Prokasova, N.V., Preparativnaya biokhimiya lipidov (Preparative Biochemistry of Lipids), Moscow: Nauka, 1981.
Zhukov, A.V., Lebedeva, N.I., and Vereshchagin, A.G., Effect of Helminthosporic Root Rot on the Lipid Content in Wheat Seedlings, Fiziol. Rast. (Moscow), 2001, vol. 48, pp. 132–136 (Russ. J. Plant Physiol., Engl. Transl.).
Stahl, E., Thin-Layer Chromatography. A Laboratory Handbook, New York: Springer-Verlag, 1969.
Taylor, D.C., MacKenzie, S.L., McCurdy, A.R., McVetty, P.B.E., Giblin, E.M., Pass, E.W., Stone, S.J., Scarth, R., Rimmer, S.R., and Pickard, M.D., Stereospecific Analyses of Seed Triacylglycerols from High-Erucic Acid Brassicaceae: Detection of Erucic Acid at the sn-2 Position in Brassica oleracea L. Genotypes, J. Am. Oil Chem. Soc., 1994, vol. 71, pp. 163–167.
Zhukov, A.V. and Vereshchagin, A.G., Contemporary Methods of Extraction, Purification, and Preliminary Fractionation of Plant Polar Lipids, Fiziol. Rast. (Moscow), 1980, vol. 27, pp. 171–187 (Sov. Plant Physiol., Engl. Transl.).
Thomas, A.E.,III., Sharoun, J.E., and Ralston, H., Quantitative Estimation of Isomeric Monoglycerides by TLC, J. Am. Oil Chem. Soc., 1965, vol. 42, pp. 789–792.
Gunstone, F.D. and Qureshi, M.I., Glyceride Studies. Part IV. The Component Glycerides of Ten Seed Oils Containing Linoleic Acid, J. Am. Oil Chem. Soc., 1965, vol. 42, pp. 961–965.
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Pchelkin, V.P., Kuznetsova, E.I., Tsydendambaev, V.D. et al. Determination of the Positional-Species Composition of Plant Reserve Triacylglycerols by Partial Chemical Deacylation. Russian Journal of Plant Physiology 48, 701–707 (2001). https://doi.org/10.1023/A:1016732708350
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DOI: https://doi.org/10.1023/A:1016732708350