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Characterization of the Arabidopsis lecRK-a genes: members of a superfamily encoding putative receptors with an extracellular domain homologous to legume lectins

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Abstract

An Arabidopsis cDNA clone that defines a new class of plant serine/threonine receptor kinases was found to be a member of a family of four clustered genes (lecRK-a1–a4) which have been cloned, sequenced and mapped on chromosome 3. This family belongs to a large superfamily encoding putative receptors with an extracellular domain homologous to legume lectins and appears to be conserved at least among dicots. In the Columbia ecotype only the lecRK-a1 and perhaps the lecRK-a3 gene is functional, since lecRK-a2 is disrupted by a Ty-copia retroelement and lecRK-a4 contains a frameshift mutation. Structural analysis of the lecRK-a1 and lecRK-a3 deduced amino-acid sequences suggests that the lectin domain is unlikely to be involved in binding monosaccharides but could interact with complex glycans and/or with hydrophobic ligands. Immunodetection of lecRK gene products in plasma membranes purified by free-flow electrophoresis showed that the lecRK-a proteins are probably highly glycosylated integral plasma membrane components.

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Authors and Affiliations

  1. Laboratoire de Biologie Moléculaire des Relations, Plantes-Microorganismes, UMR 215 CNRS-INRA, BP27, 31326, Castanet Tolosan cedex, France

    Christine Hervé, Jérome Serres, Patrick Dabos & Bernard Lescure

  2. Centre de Biologie et Physiologie Végétale, UMR 5546 CNRS-UPS, Université Paul Sabatier, 118 route de Narbonne, 31062, Toulouse cedex

    Hervé Canut

  3. Faculté des Sciences Pharmaceutiques, Institut de Pharmacologie et de Biologie Structurale, UPR-CNRS 9062, 205 route de Narbonne, 31077, Toulouse cedex, France

    Annick Barre & Pierre Rougé

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  1. Christine Hervé
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  2. Jérome Serres
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  7. Bernard Lescure
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Hervé, C., Serres, J., Dabos, P. et al. Characterization of the Arabidopsis lecRK-a genes: members of a superfamily encoding putative receptors with an extracellular domain homologous to legume lectins. Plant Mol Biol 39, 671–682 (1999). https://doi.org/10.1023/A:1006136701595

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