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Mitochondrial malate dehydrogenases in Brassica napus: altered protein patterns in different nuclear mitochondrial combinations

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Abstract

Two-dimensional analyses of mitochondrial proteins of Brassica napus revealed a set of differences in patterns of mitochondrial matrix proteins isolated from different nuclear backgrounds. One of these varying proteins was identified as mitochondrial malate dehydrogenase (mMDH; EC 1.1.1.37) by homology analyses of the partial amino acid sequence. Immunological detection identified additional mMDH subunits and detected different patterns of mMDH subunits in two distinct mitochondria types although they were isolated from plants with the same nuclear genotype. These differences are also reflected in isozym patterns, whereas Southern analyses showed no alteration in genome structure. Therefore mitochondria type-specific mMDH modifications are possible.

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Author notes

  1. Uwe Witt & Renate Lührs

    Present address: MPI, Karl Liebknechtstrasse 25, 14476, Golm, Germany

Authors and Affiliations

  1. Institut für Allgemeine Botanik, Universität Hamburg, Ohnhorststrasse 18, 22609, Hamburg, Germany

    Uwe Witt, Renate Lührs, Kerstin Lembke, Marlies Grüneberg-Seiler & Wolfgang Abel

  2. Institut für Zellbiochemie und klinische Neuro-biologie, Universitätskrankenhaus Eppendorf, Martinistrasse 52, 20251, Hamburg, Germany

    Friedrich Buck

Authors
  1. Uwe Witt
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  2. Renate Lührs
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  5. Marlies Grüneberg-Seiler
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Witt, U., Lührs, R., Buck, F. et al. Mitochondrial malate dehydrogenases in Brassica napus: altered protein patterns in different nuclear mitochondrial combinations. Plant Mol Biol 35, 1015–1021 (1997). https://doi.org/10.1023/A:1005969620157

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