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Hsp 12 is a LEA-like protein in Saccharomyces cerevisiae

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Abstract

LEA group I, II and III antibodies all recognised soluble proteins present in an extract of yeast (Saccharomyces cerevisiae). The smaller protein of the two recognised by the group I antibody displayed identical migration on SDS-PAGE to the pea seed LEA group I protein against which the antibody was raised. However, the antibody failed to recognise the predominant protein present after heating the extract at 80 °C for 10 min. This predominant protein, which also displayed identical migration on SDS-PAGE, was purified from the supernatant of the extract heated at 80 °C for 10 min. Peptide sequencing after CNBr cleavage identified the isolated protein as the heat shock protein HSP 12. Despite a previous report that HSP 12 is a heat shock protein, HSP 12 was found to increase in yeast grown at 37 °C compared with growth at 30 °C . However, increased amounts of HSP 12 were present in yeast after entry into stationary phase; this was enhanced by growth in the osmolytes NaCl and mannitol.

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Authors and Affiliations

  1. Department of Biochemistry, University of Cape Town, Private Bag, Rondebosch, 7701, South Africa

    Linda Mtwisha, Wolf Brandt, Sue McCready & George G. Lindsey

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  1. Linda Mtwisha
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  2. Wolf Brandt
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  3. Sue McCready
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  4. George G. Lindsey
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Mtwisha, L., Brandt, W., McCready, S. et al. Hsp 12 is a LEA-like protein in Saccharomyces cerevisiae. Plant Mol Biol 37, 513–521 (1998). https://doi.org/10.1023/A:1005904219201

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