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Wound and ethylene induction of the ACC oxidase melon gene CM-ACO1 occurs via two direct and independent transduction pathways

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Abstract

The enzyme ACC oxidase catalyses the last step of ethylene biosynthesis in plants. Expression of the melon ACC oxidase gene, CM-ACO1, is rapidly induced (within 10 min) by ethylene treatment or upon wounding in leaves. The inhibitor of ethylene action, 1-methylcyclopropene (1-MCP), inhibited the accumulation of ethylene-induced CM-ACO1 mRNA transcripts, while wound-induced expression of the gene was not affected. The 5′-untranslated region of the CM-ACO1 gene was fused to the β-glucuronidase (GUS) reporter gene and the corresponding transgenic tobacco plants were analysed. Two separate regions of the CM-ACO1 promoter activated GUS expression in response to ethylene treatment and wounding. These results suggest that induction of CM-ACO1 gene expression occurs via two separate signal transduction pathways in response to wounding and ethylene treatment.

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Authors and Affiliations

  1. Laboratoire Ethylène et Mécanismes Moléculaires de la Maturation des Fruits UA-INRA, Ecole Nationale Supérieure Agronomique de Toulouse, 145 Avenue de Muret, 31076, Toulouse cedex 3, France

    Thomas Bouquin, Eric Lasserre, Jérôme Pradier, Jean-Claude Pech & Claudine Balagué

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  1. Thomas Bouquin
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  2. Eric Lasserre
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  3. Jérôme Pradier
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  4. Jean-Claude Pech
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  5. Claudine Balagué
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Bouquin, T., Lasserre, E., Pradier, J. et al. Wound and ethylene induction of the ACC oxidase melon gene CM-ACO1 occurs via two direct and independent transduction pathways. Plant Mol Biol 35, 1029–1035 (1997). https://doi.org/10.1023/A:1005902226054

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