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QTL mapping for some grain traits in bread wheat (Triticum aestivum L.)

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Abstract

Grain traits are important agronomic attributes with the market value as well as milling yield of bread wheat. In the present study, quantitative trait loci (QTL) regulating grain traits in wheat were identified. Data for grain area size (GAS), grain width (GWid), factor form density (FFD), grain length-width ratio (GLWR), thousand grain weight (TGW), grain perimeter length (GPL) and grain length (GL) were recorded on a recombinant inbred line derived from the cross of NW1014 × HUW468 at Meerut and Varanasi locations. A linkage map of 55 simple sequence repeat markers for 8 wheat chromosomes was used for QTL analysis by Composite interval mapping. Eighteen QTLs distributed on 8 chromosomes were identified for seven grain traits. Of these, five QTLs for GLWR were found on chromosomes 1A, 6A, 2B, and 7B, three QTLs for GPL were located on chromosomes 4A, 5A and 7B and three QTLs for GAS were mapped on 5D and 7D. Two QTLs were identified on chromosomes 4A and 5A for GL and two QTLs for GWid were identified on chromosomes 7D and 6A. Similarly, two QTLs for FFD were found on chromosomes 1A and 5D. A solitary QTL for TGW was identified on chromosome 2B. For several traits, QTLs were also co-localized on chromosomes 2B, 4A, 5A, 6A, 5D, 7B and 7D. The QTLs detected in the present study may be validated for specific crosses and then used for marker-assisted selection to improve grain quality in bread wheat.

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Acknowledgements

The authors like to thank The Head, Department of Genetics and Plant Breeding, CCS University (Meerut, India) for providing facilities. PKG and HSB were each awarded the position of INSA Senior Scientist. PKG was also awarded a National Academy of Sciences India (NASI) Senior Scientist Platinum Jubilee Fellowship during the tenure of this research work. SK and VJ each were awarded a JRF/SRF in research projects sanctioned by the Department of Biotechnology, Government of India, New Delhi. Primer aliquots for 17 SSRs provided by Dr. M.S Röder, Leibniz Institute of Plant Genetics and Crop Plant Research, Gatersleben, Germany, is gratefully acknowledged.

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  1. Molecular Biology Laboratory, Department of Genetics and Plant Breeding, Ch. Charan Singh University, Meerut, U.P., India

    Supriya Kumari, Vandana Jaiswal, Harindra Singh Balyan & Pushpendra Kumar Gupta

  2. School of Life Science, Jawaharlal Nehru University, New Delhi, India

    Vandana Jaiswal

  3. Department of Genetics and Plant Breeding, Institute of Agricultural Sciences, Banaras Hindu University, Varanasi, U.P., India

    Vinod Kumar Mishra

  4. International Institute of Tropical Agriculture (IITA), Ibadan, PMB 5320, Nigeria

    Rajneesh Paliwal

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Kumari, S., Jaiswal, V., Mishra, V.K. et al. QTL mapping for some grain traits in bread wheat (Triticum aestivum L.). Physiol Mol Biol Plants 24, 909–920 (2018). https://doi.org/10.1007/s12298-018-0552-1

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