Abstract
The contents of dopamine, serotonin, and noradrenaline in rat fetuses developing under the conditions of their deficiency induced by administration of α-methyl-para-tyrosine to females during 11th to 16th or 20th day of pregnancy and in fetuses, whose mothers were given saline at the same time, were determined using HPLC with subsequent electrochemical detection. Administration of α-methyl-para-tyrosine led to decreased levels of dopamine and noradrenaline in the areas of migration of GnRH-neurons in fetuses on days 17 and 21 of prenatal development. The concentration of serotonin remained unchanged, except in the head nasal area in males on day 21. The areas of interaction between the brain catecholaminergic systems and migrating and differentiating GnRH-neurons were determined by double immunohistochemical labeling. Close topographical location of GnRH-immunoreactive neurons and tyrosine hydroxylase-immunoreactive in the area of nucleus accumbens on days 17 and 20, as well as in the median eminence on day 20. The GnRH concentration in the caudal areas of migration of GnRH-neurons under the normal conditions and in the case of catecholamine deficiency was determined using radioimmunoassay. After administration of α-methyl-para-tyrosine the GnRH concentration in the anterior hypothalamus decreased in females. The data obtained suggest the involvement of catecholamines in the regulation of development of GnRH-Neurons during prenatal development. In addition, the adequacy and efficiency of the used model of catecholamine deficiency for studying the development of such neurons was confirmed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
REFERENCES
Adamskaya, E.I., Kuznetsova, T.A., Shishkina, I.V., et al., Interaction between Regulatory Effects of Serotonin and Testosterone on Development of the LHRH-producing System in the Rat Brain during Prenatal Period, Ontogenez, 1998, vol. 29, no.1, pp. 47–51.
Bernabe, J., Proshlyakova, E.V., Sapronova, A.Y., et al., Pharmacological Model of Catecholamine Depletion in the Hypothalamus of Fetal and Neonatal Rats and Its Application, Cell. Mol. Biol., 1996, vol. 16, no.6, pp. 617–624.
Bless, E.P., Westaway, W.A., Schwarting, G.A., and Tobet, S.A., Effects of Gamma-Aminobutyric Acid (A) Receptor Manipulation on Migrating Gonadotropin-Releasing Hormone Neurons Through the Entire Migratory Route in vivo and in vitro, Endocrinology, 2000, vol. 141, no.3, pp. 1254–1262.
Brodie, B.B., Costa, E., Dlabac, A., et al., Application of Steady-State Kinetics of the Estimation of Synthesis and Turnover Time of Tissue Catecholamines, J. Pharm. Exp. Ter., 1966, vol. 154, pp. 493–498.
Caldani, M., Batailler, M., Thiery, J.C., and Dubois, M.P., LHRH-Ir Structures in the Sheep Brain, J. Histochem., 1988, vol. 89, pp. 129–139.
Daikoku-Ishido, H., Okamura, Y., Yanaihara, M., and Daikoku, S., Development of the Hypothalamic Luteinizing Hormone-Releasing Hormone-Containing Neuron System in the Rat: In vivo and Transplantation Studies, Devel. Biol., 1990, vol. 140, pp. 347–387.
Fueshko, S.M., Key, S., and Wray, S., GABA Inhibits Migration of Luteinizing Hormone-Releasing Hormone Neurons in Embryonic Olfactory Explants, J. Neurosci., 1998, vol. 18, no.7, pp. 2560–2569.
Hokfelt, T., Martensson, R., and Bjorklund, A., Distribution Maps of Tyrosine Hydroxylase-Immunoreactive Neurons in the Rat Brain, Handbook of Chemical Anatomy, Classical Transmitters in the CNS, New York: Elsevier, 1984, vol. 1.
Horvath, T.L., Naftolin, F., and Leranth, C., Luteinizing Hormone-Releasing Hormone and Gamma-Aminobutyric Acid Neurons in the Medial Preoptic Area Are Synaptic Targets of Dopamine Axons Originating in Anterior Periventricular Areas, J. Neuroendocrinol., 1993, vol. 5, no.1, pp. 71–79.
Hosny, S. and Jennes, L., Identification of 1B Adrenergic Receptors in Gonadotropin Releasing Hormone Neurons of the Female Rat, Neuroendocrinology, 1998, vol. 10, pp. 687–692.
Izvolskaya, M.S., Voronova, S.N., Makarenko, I.G., et al., Effects of Catecholamines on Migration and Differentiation of GRH-Neurons in Rats, Ontogenez, 2004, vol. 35, no.1, pp. 23–32.
Jeffcoate, S.L., Flaser, H.M., Holland, D.T., and Gunn, A., Radioimmunoassay of Luteinizing Hormone-Releasing Hormone (LH-RH) in Serum from Man, Sheep and Rat, Acta Endocrinol. (Copenh.), 1974, vol. 75, pp. 625–635.
Jennes, L., Beckman, W.C., Stumpf, W.E., and Grzanna, R., Anatomical Relations of Serotoninergic and Noradrenalinergic Projections with the GnRH System in Septum and Hypothalamus, Exp. Brain Res., 1982, vol. 46, pp. 331–338.
Jennes, L., Stumpf, W.E., and Tappaz, M.L., Anatomical Relationships of Dopaminergic and GABAergic System with the GnRH-System in the Septo-Preoptic Area, Exp. Brain Res., 1983, vol. 50, pp. 90–91.
Moore, J.P. and Wray, S., Luteinizing Hormone Releasing Hormone (LHRH) Biosynthesis and Secretion in Embryonic LHRH, Endocrinology, 2000, vol. 141, no.12, pp. 4486–4495.
Pares-Herbute, N., Tapia-Arancibia, L., and Astier, H., Ontogeny of the Metencephalic, Mesencephalic and Diencephalic Content of Catecholamines as Measured by High Performance Liquid Chromatography with Electrochemical Detection, Int. J. Devel. Neurosci., 1989, vol. 7, no.1, pp. 73–79.
Pol, O., Campmany, L., and Armario, A., Inhibition of Catecholamine Synthesis with Alpha-Methyl-P-Tyrosine Apparently Increases Brain Serotoninergic Activity in the Rat: No Influence of Previous Chronic Immobilization Stress, Pharmacol. Biochem. Behav., 1995, vol. 52, no.1, pp. 107–112.
Pronina, T., Ugrumov, M., Adamskaya, E., et al., Influence of Serotonin on Differentiation and Migration of Gonadotropin-Releasing Hormone Neurons in Rat Fetuses, J. Neuroendocrinol., 2003a, vol. 15, pp. 549–558.
Pronina, T., Ugrumov, M., Calas, A., et al., Influence of Monoamines on Differentiating Gonadotropin-Releasing Hormone Neurons in Fetal Mice, J. Neuroendocrinol., 2003b, vol. 15, pp. 925–932.
Rhode, W., Ohkawa, T., Gotz, F., et al., Sex Specific Effects on the Fetal Neuroendocrine System during Acute Stress in Late Pregnancy of Rat and the Influence of a Simultaneous Treatment by Tyrosine, Exp. Clin. Endocrinol., 1983, vol. 94, nos.1–2, pp. 23–42.
Samsonova, V.M. and Babichev, V.N., Effects of Thyroid Hormones on Functional Activity of the Hypothalamo-Hypophysial Axis in Female Rats, Probl. Endokrinol. (Moscow), 1977, vol. 23, no.5, pp. 53–62.
Schwanzel-Fukuda, M. and Pfaff, D.W., Origin of Luteinizing Hormone-Releasing Hormone Neurons, Nature, 1989, vol. 338, no.6211, pp. 161–164.
Shi, S.R., Cote, R.G., and Taylor, C.R., Antigenal Retrieval Techniques: Current Perspectives, J. Hist. Cytochem., 2001, vol. 49, no.8, pp. 931–937.
Simonian, S.X. and Herbison, A.E., Regulation of Gonadotropin-Releasing Hormone (GnRH) Gene Expression During GnRH Neuron Migration in the Mouse, Neuroendcrinology, 2001, vol. 73, no.3, pp. 149–156.
Specht, L.A., Pickel, V.M., Joh, T.H., and Reis, D.J., Light-Microscopic Immunocytochemical Localization of Tyrosine Hydroxylase in Prenatal Rat Brain. 1. Early Ontogeny, J. Comp. Neurol., 1981, vol. 20, no.2, pp. 233–253.
Werner, M., Von Wasilevsky, R., and Komminoth, P., Antigen Retrieval, Signal Amplification and Intensification in Immunocytochemistry, Histochem. Cell Biol., 1996, vol. 105, no.4, pp. 253–260.
Wray, S. and Hoffman, G., Catecholamine Innervation of LH-RH Neurons: A Developmental Study, Brain Res., 1986, vol. 399, pp. 327–331.
Wray, S., Grant, P., and Gainer, H., Evidence That Cells Expressing Luteinizing Hormone-Releasing Hormone MRNA in the Mouse Are Derived from Progenitor Cells in the Olfactory Placode, Proc. Natl. Acad. Sci. USA, 1989, vol. 86, no.20, pp. 8132–8136.
Author information
Authors and Affiliations
Additional information
__________
Translated from Ontogenez, Vol. 36, No. 6, 2005, pp. 440–448.
Original Russian Text Copyright © 2005 by Izvolskaya, Adamskaya, Voronova, Duittoz, Tillet.
Rights and permissions
About this article
Cite this article
Izvolskaya, M.S., Adamskaya, E.I., Voronova, S.N. et al. Catecholamines in Regulation of Development of GnRH Neurons of Rat Fetuses. Russ J Dev Biol 36, 369–376 (2005). https://doi.org/10.1007/s11174-005-0053-3
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/s11174-005-0053-3