Abstract
Triploidy interferes with gametogenesis in all fish species tested so far. In fish it results in complete female sterility however, males are still able to develop testis. The reason why sterility levels in triploid fishes differ among species and between sexes is unclear. In the present study the reproductive capacity of triploid males of tench was studied. Flow cytometry revealed sperm cells of triploids to be largely aneuploid with high mosaic DNA, oscillating from haploid DNA to diploid DNA content. Analysis of variance showed an insignificant influence of ploidy level on the percentage of motile spermatozoa, as well as on spermatozoa velocity. Experimental crosses between normal diploid female and triploid males resulted in the appearance of triploid progeny, which exhibited genotypes composed of microsatellite alleles inherited from the founder female and additional allele derived from the donor male. We can conclude that the triploid males analysed in the present study were capable to fertilize eggs derived from diploid females.
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References
Benfey TJ (1999) The physiology and behaviour of triploid fishes. Rev Fish Sci 7:39–67
Benfey T, Solar I, de Jong G, Donaldson E (1986) Flow-cytometric confirmation of aneuploid in sperm from triploid rainbow trout. Trans Am Fish Soc 115:838–840
Collares-Pereira MJ, Madeira JM, Rab P (1995) Spontaneous triploidy in the stone loach Noemacheilus barbatulus (Balitoridae). Copeia 2:483–484
Devlin RH, Nagahama Y (2002) Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences. Aquaculture 208:191–364
Flajšhans M, Linhart O, Kvasnička P (1993) Genetic studies of tench (Tinca tinca L.): induced triploidy and tetraploidy and first performance data. Aquaculture 113:301–312
Flajšhans M, Rodina M, Halačka K, Vetešnik L, Gela D, Luskova V, Lusk S (2008) Characteristics of sperm of polyploid prussian carp carassius gibelio. J Fish Biol 73:323–328
Kohlmann K, Kersten P (2006) PMicrosatellite loci in tench: isolation and variability in a test population. Aquac Int 14:3–7
Lincoln RF (1981) The growth of female and diploid and triploid plaice (Pleuronectes platessa) × flounder (Platichthys flesus) hybrid over one spawning season. Aquaculture 25:259–268
Linhart O, Gela D, Flajšhans M, Duda P, Rodina M, Novak V (2000) Alcalase enzyme treatment for elimination of egg stickiness in tench, Tinca tinca L. Aquaculture 191:303–308
Linhart O, Rodina M, Flajšhans M, Mavrodiev N, Nebesarova J, Gela D, Kocour M (2006) Studies on sperm of diploid and triploid tench (Tinca tinca L.). Aquac Int 14:9–25
Meirmans PG, Van Tienderen PH (2004) GENOTYPE and GENODIVE: two programs for the analysis of genetic diversity of asexual organisms. Mol Ecol Notes 4:792–794
Morishima K, Horie S, Yamaha E, Arai K (2002) A cryptic clonal line of the loach Misgurnus anguillicaudatus (Teleostei: Cobitidae) evidenced by induced gynogenesis, interspecific hybridization, microsatellite genotyping and multilocus DNA fingerprinting. Zool Sci 19:565–575
Otto SP, Whitton J (2000) Polyploid incidence and evolution. Annu Rev Genet 34:401–437
Rodina M, Cosson J, Gela D, Linhart O (2004) Kurokura solution as immobilization solution for spermatozoa of tench (Tinca tinca L.). Aquac Int 12:119–131
Shen J, Fan Z, Wang G (1983) Karyotype studies of male triploid crucian carp (Fangzheng crucian carp) in Heilongjian. Acta Genet Sci 10:133–136
Stanley J, Allen SK Jr, Hidu H (1981) Polyploidy induced in the American oyster, Crassostrea virginica with cytochalasin B. Aquaculture 23:1–10
Ueda T, Sawada M, Kobayashi J (1987) Cytogenetical characteristics of embryos between diploid female and triploid male in rainbow trout. Jpn J Genet 62:461–465
Vindelov LL, Christensen IJ (1990) Review of techniques and results obtained in one laboratory by an integrated system of methods designed for routine clinical flow cytometric DNA analysis. Cytometry 11:753–770
Yamaha E, Murakami M, Hada K, Otani S, Fujimoto T, Tanaka M, Sakao S, Kimura S, Sato S, Arai K (2003) Recovery of fertility in male hybrids of a cross between goldfish and common carp by transplantation of PGC (primordial germ cell)-containing graft. Genetica 119:121–131
Acknowledgments
This study was financial supported by USB RIFCH No: MSM 6007665809 and GACR no. 524/06/0817. Authors give also thanks to two anonymous referees as well as to the Guest Editor for valuable comments on an earlier version of manuscript.
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Hulak, M., Kaspar, V., Psenicka, M. et al. Does triploidization produce functional sterility of triploid males of tench Tinca tinca (L.)?. Rev Fish Biol Fisheries 20, 307–315 (2010). https://doi.org/10.1007/s11160-009-9139-9
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DOI: https://doi.org/10.1007/s11160-009-9139-9