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The rice OsGAE1 is a novel gibberellin-regulated gene and involved in rice growth

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Abstract

Gibberellins (GAs) are a class of phytohormones that regulate many aspects of plant growth and development processes including stem elongation, flowering, and seed germination. A novel GA-enhanced gene, designated as OsGAE1, was identified using microarray analysis of GA-regulated genes. OsGAE1 expressed in a dose- and time course-dependent manner with minimum expression at 1 µM GA3 and maximum expression at 50 µM GA3 starting from 30 min and peaked at 24 h after GA3 treatment. OsGAE1 expression was up-regulated by GA3 at transcript level while no significant effect was observed for other hormones. OsGAE1 was expressed in Escherichia coli with N-terminal His6 tag and the recombinant protein migrated at 38 kDa, slightly larger than the predicted 29 kDa, during SDS-PAGE. Anti-OsGAE1 antibodies immunoreacted with a protein of 40 kDa in rice leaf sheath. OsGAE1 expressed mainly in growing leaf sheath and callus compared to leaf and root. In situ hybridization and OsGAE1 promoter analysis revealed that OsGAE1 expressed in shoot apex meristem and young primary leaves. Northern blot, Western blot, and GUS activities revealed that OsGAE1 is up-regulated by GA3. Transgenic rice expressing OsGAE1 in antisense orientation exhibited severely affected vegetative and reproductive growth. The transgenic plants were 55–70% short compared to control. These results suggest that OsGAE1 is differentially expressed in rice␣leaf sheath in relation to GA3 and it encodes a functional protein which is involved in GA-regulated growth and development of rice.

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Acknowledgments

The authors are grateful to Dr T. Asano for his valuable discussion. We are also thankful to Dr M. Nakamura and Dr H. Ichikawa for their valuable help. Special thanks are extended to Mr H. Takasaki for his help in MS/MS analysis. We are grateful Dr E.E. Hood for providing Agrobacterium strain EHA105. Special thanks are due to Dr Y. Ohashi for letting us use her microscopic facilities. The full-length cDNA was provided by NIAS DNA Bank. This work was supported by the Program of BRAIN and Rice Genome Project from the Ministry of Agriculture, Forestry and Fisheries, Japan.

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Authors and Affiliations

  1. Department of Molecular Biology, National Institute of Agrobiological Sciences, 2-1-2 Kannondai, Tsukuba, 305-8602, Japan

    Asad Jan & Setsuko Komatsu

  2. Institute of Applied Biochemistry, School of Life and Environmental Sciences, University of Tsukuba, Tsukuba , 305-8572, Japan

    Asad Jan & Hiroshi Matsumoto

  3. Nagoya University, Nagoya, 464-8601, Japan

    Hidemi Kitano

  4. National Institute of Crop Science, 2-1-18 Kannondai, Tsukuba, 305-8518, Japan

    Setsuko Komatsu

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  1. Asad Jan
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  2. Hidemi Kitano
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Correspondence to Setsuko Komatsu.

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Jan, A., Kitano, H., Matsumoto, H. et al. The rice OsGAE1 is a novel gibberellin-regulated gene and involved in rice growth. Plant Mol Biol 62, 439–452 (2006). https://doi.org/10.1007/s11103-006-9030-1

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  • DOI: https://doi.org/10.1007/s11103-006-9030-1

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