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CENTRIN2 Interacts with the Arabidopsis Homolog of the Human XPC Protein (AtRAD4) and Contributes to Efficient Synthesis-dependent Repair of Bulky DNA Lesions

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Abstract

Arabidopsis thaliana CENTRIN2 (AtCEN2) has been shown to modulate Nucleotide Excision Repair (NER) and Homologous Recombination (HR). The present study provides evidence that AtCEN2 interacts with the Arabidopsis homolog of human XPC, AtRAD4 and that the distal EF-hand Ca2+ binding domain is essential for this interaction. In addition, the synthesis-dependent repair efficiency of bulky DNA lesions was enhanced in cell extracts prepared from Arabidopsis plants overexpressing the full length AtCEN2 but not in those overexpressing a truncated AtCEN2 form, suggesting a role for the distal EF-hand Ca2+ binding domain in the early step of the NER process. Upon UV-C treatment the AtCEN2 protein was shown to be increased in concentration and to be localised in the nucleus rapidly. Taken together these data suggest that AtCEN2 is a part of the AtRAD4 recognition complex and that this interaction is required for efficient NER. In addition, NER and HR appear to be differentially modulated upon exposure of plants to DNA damaging agents. This suggests in plants, that processing of bulky DNA lesions highly depends on the excision repair efficiency, especially the recognition step, thus influencing the recombinational repair pathway.

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Abbreviations

BER:

Base Excision Repair

DSB:

Double Strand Break

GFP:

Green Fluorescent Protein

HR:

Homologous Recombination

ICL:

Inter/Intra Crosslink

NHEJ:

Non Homologous End Joining

NER:

Nucleotide Excision Repair

MMR:

Mismatch Repair

XP:

Xeroderma Pigmentosum

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Authors and Affiliations

  1. Plant Biochemistry Physiology Group, Federal Institute of Technology (ETH) Zurich, Institute of Plant Sciences, Experimental Station Eschikon 33, CH-8315, Lindau, Switzerland

    Lu Liang

  2. Friedrich Miescher Institute, Maulbeerstrasse 66, CH 4058, Basel, Switzerland

    Véronique Kalck, Barbara Hohn & Jean Molinier

  3. Swiss Federal Institute for Environmental Science & Technology (EAWAG), Limnogical Research, Ueberlandstrasse 133, CH-8600, Duebendorf, Switzerland

    Sabine Flury

  4. Institut de Biologie Moléculaire des Plantes, 12 rue du Général Zimmer, 67084, Strasbourg, Cedex, France

    Jean Molinier

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  1. Lu Liang
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  2. Sabine Flury
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  3. Véronique Kalck
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  4. Barbara Hohn
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  5. Jean Molinier
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Correspondence to Jean Molinier.

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Liang, L., Flury, S., Kalck, V. et al. CENTRIN2 Interacts with the Arabidopsis Homolog of the Human XPC Protein (AtRAD4) and Contributes to Efficient Synthesis-dependent Repair of Bulky DNA Lesions. Plant Mol Biol 61, 345–356 (2006). https://doi.org/10.1007/s11103-006-0016-9

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