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AtSAP18, An Orthologue of Human SAP18, is Involved in the Regulation of Salt Stress and Mediates Transcriptional Repression in Arabidopsis

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Abstract

In yeast and mammalian systems, it is well established that transcriptional down-regulation by DNA-binding repressors involves core histone deacetylation, mediated by their interaction within a complex containing histone deacetylase (e.g. HDA1), as well as various other proteins (e.g. SIN3, SAP18, SAP30, and RbAp46). Here we identify that a Arabidopsis thaliana gene related in sequence to SAP18, designated AtSAP18, functions in transcription regulation in plants subjected to salt stress. The AtSAP18 loss- of-function mutant is more sensitive to NaCl, and is impaired in chlorophyll synthesis as compared to the wild-type. Using GST pull-down, two-hybrid, and transient transcription assays, we have characterized SAP18 and HDA1 orthologues and provide evidence that SAP18 and HDA1 function as transcriptional repressors. We further demonstrate that they associate with Ethylene-Responsive Element binding Factors (ERFs) to create a hormone-sensitive multimeric repressor complex under conditions of environmental stress. Our results indicate that AtSAP18 functions to link the HDA complex to transcriptional repressors that are bound to chromatin in a sequence-specific manner, thereby providing the specificity of signal transduction accompanying transcriptional repression under stress conditions.

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Abbreviations

HDA:

histone deacetylase

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  1. Department of Plant Sciences, University of Arizona, 303 Forbes Building, Tucson, AZ, 85721, USA

    Chun-Peng Song & David W. Galbraith

  2. Laboratory of Plant Stress Biology, College of Life Sciences, Henan University, 475001, Kaifeng, China

    Chun-Peng Song

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  1. Chun-Peng Song
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Song, CP., Galbraith, D.W. AtSAP18, An Orthologue of Human SAP18, is Involved in the Regulation of Salt Stress and Mediates Transcriptional Repression in Arabidopsis. Plant Mol Biol 60, 241–257 (2006). https://doi.org/10.1007/s11103-005-3880-9

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