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Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling

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Abstract

Fructose-bisphosphate aldolase is a glycolytic enzyme whose activity increases in rice roots treated with gibberellin (GA). To investigate the relationship between aldolase and root growth, GA-induced root aldolase was characterized. GA3 promoted an increase in aldolase accumulation when 0.1 μ M GA3 was added exogenously to rice roots. Aldolase accumulated abundantly in roots, especially in the apical region. To examine the effect of aldolase function on root growth, transgenic rice plants expressing antisense aldolase were constructed. Root growth of aldolase-antisense transgenic rice was repressed compared with that of the vector control transgenic rice. Although aldolase activity increased by 25% in vector control rice roots treated with 0.1 μ M GA3, FBPA activity increased very little by 0.1 μ M GA3 treatment in the root of aldolase-antisense transgenic rice. Furthermore, aldolase co-immunoprecipitated with antibodies against vacuolar H+-ATPase in rice roots. In the root of OsCDPK13-antisense transgenic rice, aldolase did not accumulate even after treatment with GA3. These results suggest that the activation of glycolytic pathway function accelerates root growth and that GA3-induced root aldolase may be modulated through OsCDPK13. Aldolase physically associates with vacuolar H-ATPase in roots and may regulate the vacuolar H-ATPase mediated control of cell elongation that determines root length.

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Abbreviations

CBB:

Comassie brilliant blue

CDPK:

Ca2+-dependent protein kinase

GA:

gibberellin

V-ATPase:

vacuolar H2+-ATPase

References

  • F. Abbasi H. Onodera S. Toki H. Tanaka S. Komatsu (2004) ArticleTitleOsCDPK13, a calcium-dependent protein kinase gene from riceis induced by cold and gibberellin in rice leaf sheath Plant Mol. Biol. 55 541–552

    Google Scholar 

  • G.S. Bailey, J.M. Walker, (1985) The production of antisera Methods in Molecular Biology Humana Press TotowaNJ 295--300

  • M.M. Burrell P.J. Mooney M. Blundy D. Carter F. Wilson J. Green K.S. Blundy T. ap Rees (1994) ArticleTitleGenetic manipulation of 6-phosphofructokinase in potato tubers Planta 194 95–101

    Google Scholar 

  • F. Chaumont F. Barrieu E.M. Herman M.J. Chrispeels (1998) ArticleTitleCharacterization of a maize tonoplast aquaporin expressed in zones of cell division and elongation Plant Physiol. 117 1143–1152

    Google Scholar 

  • N.H. Cheng J.K. Pitman Barkla B.J. Shigaki T. Hirchi K.D. (2003) ArticleTitleThe Arabidopsis cax1 mutant exhibits impaired ion homeostasis, development and hormonal responses and reveals interplay among vacuolar transporters Plant Cell 15 347–364 Occurrence Handle10.1105/tpc.007385 Occurrence Handle1:CAS:528:DC%2BD3sXhtlOmtb4%3D Occurrence Handle12566577

    Article  CAS  PubMed  Google Scholar 

  • P. Chomczynski N. Sacchi (1987) ArticleTitleSingle-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction Anal. Biochem. 162 156–159 Occurrence Handle10.1006/abio.1987.9999 Occurrence Handle1:CAS:528:DyaL2sXitFSns7Y%3D Occurrence Handle2440339

    Article  CAS  PubMed  Google Scholar 

  • L. Dolan J. Davies (2004) ArticleTitleCell expansion in roots Curr. Opin. Plant Biol. 7 33–39

    Google Scholar 

  • A.R. Fernie F. Carrari L.J. Sweetlove (2004) ArticleTitleRespiratory metabolism:glycolysis, the TCA cycle and mitochondrial electron transport Curr. Opin. Plant Biol. 7 254–261

    Google Scholar 

  • S. Gilroy R.L. Jones (1992) ArticleTitleGibberellic acid and abscisic acid coordinately regulate cytoplasmic calcium and secretory activity in barley aleurone protoplasts Proc. Natl. Acad. Sci. USA 89 3591–3595

    Google Scholar 

  • J.P. Gogarten J. Fichmann Y. Braun L. Morgan P. Styles S.L. Taiz K. de Lapp L. Taiz (1992) ArticleTitleThe use of antisense mRNA to inhibit the tonoplast H-ATPase in carrot Plant Cell 4 851–864 Occurrence Handle10.1105/tpc.4.7.851 Occurrence Handle1:CAS:528:DyaK38Xlt1yhtrs%3D Occurrence Handle1392599

    Article  CAS  PubMed  Google Scholar 

  • V. Haake R. Zrenner U. Sonnewald M. Stitt (1998) ArticleTitleA moderate decrease of plastid aldolase activity inhibits photosynthesis, alters the levels of sugars and starchand inhibits growth of potato plants Plant J. 14 147–157

    Google Scholar 

  • P. Hedden W.M. Proebsting (1999) ArticleTitleGenetic analysis of gibberellin biosynthesis Plant Physiol. 119 365–370

    Google Scholar 

  • Y. Hiei S. Ohta T. Komari T. Kumashiro (1994) ArticleTitleEfficient transformation of rice (Oryza sativa L.) mediated by Agrobacterium and sequence analysis of the boundaries of the T-DNA Plant J. 6 271–282

    Google Scholar 

  • B.L. Horecker (1975) ArticleTitleFructose bisphosphate aldolase from spinach Methods Enzymol. 42 234–239

    Google Scholar 

  • T. Jacobs (1997) ArticleTitleWhy do plant cells divide? Plant Cell 9 1021–1029

    Google Scholar 

  • H. Kende J.A.D. Zeevaart (1997) ArticleTitleThe five “classical” plant hormones Plant Cell 9 1197–1210 Occurrence Handle10.1105/tpc.9.7.1197 Occurrence Handle1:CAS:528:DyaK2sXlt1Shtbk%3D Occurrence Handle12237383

    Article  CAS  PubMed  Google Scholar 

  • H. Konishi, H. Kitano, S. Komatsu (1999) Identification of rice root proteins regulated by gibberellin using proteome analysis Plant Cell Environ.

  • H.G. Lebherz M.M. Leadbetter R.A. Bradshaw (1984) ArticleTitleIsolation and characterization of cytosolic and chloroplastic forms of spinach leaf fructose diphosphate aldolase J. Biol. Chem. 259 1011–1017

    Google Scholar 

  • M. Lu Y.Y. Sautin L.S. Holliday S.L. Gluck (2004) ArticleTitleThe glycolytic enzyme aldolase mediates assembly, expression, and activity of vacuolar H-ATPase J. Biol. Chem. 279 8732–8739

    Google Scholar 

  • J. Lynch (1995) ArticleTitleRoot architecture and plant productivity Plant Physiol. 109 7–13

    Google Scholar 

  • A.G. McCubbin S.M. Ritchie S.J. Swanson S. Gilroy (2004) ArticleTitleThe calcium-dependent protein kinase HvCDPK1 mediates the gibberellic acid response of the barley aleurone through regulation of vacuolar function Plant J. 39 206–218

    Google Scholar 

  • M. McCully (1995) ArticleTitleHow do real roots work? Plant Physiol. 109 1–6

    Google Scholar 

  • S. Ohta S. Mita T. Hattori K. Nakamura (1990) ArticleTitleConstruction and expression in tobacco of a β-glucuronidase (GUS) reporter gene containing an intron within the coding sequence Plant Cell Physiol. 31 805–813

    Google Scholar 

  • K. Ranathunge L. Kotula E. Steudle R. Lafitte (2004) ArticleTitleWater permeability and reflection coefficient of the outer part of young rice roots are differently affected by closure of water channels (aquaporins) or blockage of apoplastic pores J. Exp. Bot. 55 433–447

    Google Scholar 

  • F. Rolland J. Winderickx J.M. Thevelein (2001) ArticleTitleGlucose-sensing mechanisms in eukaryotic cells Trends Biochem. Sci. 26 310–317

    Google Scholar 

  • W.J. Rutter (1964) ArticleTitleEvolution of aldolase Fed. Proc. Fed. Amer. Soc. Exp. Biol. 23 1248–1257

    Google Scholar 

  • T. Sakamoto K. Miura H. Itoh T. Tatsumi M. Ueguchi-Tanaka K. Ishiyama M. Kobayashi G.K. Agrawal S. Takeda K. Abe A. Miyao H. Hirochika H. Kitano M. Ashikari M. Matsuoka (2004) ArticleTitleAn overview of gibberellin metabolism enzyme genes and their related mutants in rice Plant Physiol. 134 1642–1653

    Google Scholar 

  • G.W. Schaeffer F.T. Sharpe R.C. Sicher (1997) ArticleTitleFructose 1,6-bisphosphate aldolase activity in leaves of a rice mutant selected for enhanced lysine Phytochemistry 46 1335–1338

    Google Scholar 

  • J.W. Schiefelbein P.N. Benfey (1991) ArticleTitleThe development of plant roots:new approaches to underground problems Plant Cell 3 1147–1154

    Google Scholar 

  • K. Schumacher D. Vafeados M. McCarthy H. Sze T. Wilkins J. Chory (1999) ArticleTitleThe Arabidopsis det3 mutant reveals a central role for the vacuolar H -ATPase in plant growth and development Genes. Dev. 13 3259–3270

    Google Scholar 

  • N. Tanaka H. Konishi M.M.K. Khan S. Komatsu (2004a) ArticleTitleProteome analysis of rice tissues by two-dimensional electrophoresis:an approach to the investigation of gibberellin regulated proteins Mol. Gen. Genomics 270 485–496

    Google Scholar 

  • N. Tanaka M. Fujita H. Handa S. Murayama M. Uemura Y. Kawamura T. Mitsui S. Mikami Y. Tozawa T. Yoshinaga S. Komatsu (2004b) ArticleTitleProteomics of the rice cell:systematic identification of the protein populations in subcellular compartments Mol. Gen. Genomics 271 566–576

    Google Scholar 

  • E. Tanimoto (1994) ArticleTitleInteraction of gibberellin A3ancymidol in the growth and cell-wall extensibility of dwarf pea roots Plant Cell Physiol. 35 1019–1028

    Google Scholar 

  • S. Yamaguchi Y. Kamiya (2000) ArticleTitleGibberellin biosynthesis:Its regulation by endogenous and environmental signals Plant Cell Physiol. 41 251–257

    Google Scholar 

  • R.N. Trethewey A.R. Fernie A. Bachmann H. Fleischer-Notter P. Geigenberger L. Willmitzer (2001) ArticleTitleExpression of a bacterial sucrose phosphorylase in potato tubers results in a glucose-independent induction of glycolysis Plant Cell Environ. 24 357–365

    Google Scholar 

  • G. Yang S. Shen S. Yang S. Komatsu (2003) ArticleTitleOsCDPK13, a calcium-dependent protein kinase gene from riceis induced in response to cold and gibberellin Plant Physiol. Biochem. 41 369–374

    Google Scholar 

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Authors and Affiliations

  1. Department of Molecular Genetics, National Institute of Agrobiological Sciences, Tsukuba, Ibaraki, 305-8602, Japan

    Hirosato Konishi & Setsuko Komatsu

  2. Biotechnology Research Center, The University of Tokyo, Bunkyo-ku, Tokyo, 113-8657, Japan

    Hisakazu Yamane

  3. Laboratory of Cell Dynamics, Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya, Aichi, 464-8601, Japan

    Masayoshi Maeshima

Authors
  1. Hirosato Konishi
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  2. Hisakazu Yamane
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  3. Masayoshi Maeshima
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  4. Setsuko Komatsu
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Correspondence to Setsuko Komatsu.

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Konishi, H., Yamane, H., Maeshima, M. et al. Characterization of fructose-bisphosphate aldolase regulated by gibberellin in roots of rice seedling. Plant Mol Biol 56, 839–848 (2004). https://doi.org/10.1007/s11103-004-5920-2

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  • DOI: https://doi.org/10.1007/s11103-004-5920-2

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