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Plants of Brazilian restingas with tripanocide activity against Trypanosoma cruzi strains

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Abstract

Chagas disease is caused by the Trypanosoma cruzi affecting millions of people, and widespread throughout Latin America. This disease exhibits a problematic chemotherapy. Benznidazole, which is the drug currently used as standard treatment, lamentably evokes several adverse reactions. Among other options, natural products have been tested to discover a novel therapeutic drug for this disease. A lot of plants from the Brazilian flora did not contain studies about their biological effects. Restinga de Jurubatiba from Brazil is a sandbank ecosystem poorly studied in relation to plant biological activity. Thus, three plant species from Restinga de Jurubatiba were tested against in vitro antiprotozoal activity. Among six extracts obtained from leaves and stem parts and 2 essential oils derived from leave parts, only 3 extracts inhibited epimastigote proliferation. Substances present in the extracts with activity were isolated (quercetin, myricetin, and ursolic acid), and evaluated in relation to antiprotozoal activity against epimastigote Y and Dm28 Trypanosoma cruzi strains. All isolated substances were effective to reduce protozoal proliferation. Essentially, quercetin and myricetin did not cause mammalian cell toxicity. In summary, myricetin and quercetin molecule can be used as a scaffold to develop new effective drugs against Chagas’s disease.

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Acknowledgements

We are greatful to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), Fundação de Auxílio à Pesquisa do Estado do Rio de Janeiro (FAPERJ), Programa Institucional de Indução a Ciência, Tecnologia e Inovação em Saúde (PAPES VI) for the financial support.

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Authors and Affiliations

  1. Laboratório de Toxoplasmose e outras Protozooses– Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil

    Robson Xavier Faria

  2. Laboratório de Bioquímica de Peptídeos– Instituto Oswaldo Cruz, Fundação Oswaldo Cruz, Rio de Janeiro, Brazil

    André Luis Almeida Souza

  3. Laboratório de Tecnologia de Produtos Naturais - Departamento de Tecnologia Farmacêutica, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil

    Barbara Lima, Luis Armando Candido Tietbohl & Leandro Rocha

  4. Laboratório de Pesquisa em Fármacos – Departamento de Ciências Biológicas e da Saúde, Universidade Federal do Amapá, Macapá, Brazil

    Caio Pinho Fernandes

  5. Laboratório de Nanobiotecnologia Fitofarmacêutica – Curso de Ciências Farmacêuticas, Universidade Federal do Amapá, Macapá, Brazil

    Caio Pinho Fernandes & Raquel Rodrigues Amaral

  6. Laboratório Universitário Rodolpho Albino – Pró-Reitoria de Extensão, Universidade Federal Fluminense, Niterói, Rio de Janeiro, Brazil

    Bettina Monika Ruppelt

  7. Departamento de Ciências, Faculdade de Formação de Professores, Universidade do Estado do Rio de Janeiro, São Gonçalo, RJ, Brazil

    Marcelo Guerra Santos

Authors
  1. Robson Xavier Faria
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  2. André Luis Almeida Souza
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  3. Barbara Lima
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  4. Luis Armando Candido Tietbohl
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  5. Caio Pinho Fernandes
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  6. Raquel Rodrigues Amaral
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  7. Bettina Monika Ruppelt
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  8. Marcelo Guerra Santos
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  9. Leandro Rocha
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Contributions

Robson Xavier Faria and André Luis Souza designed the research; Leandro Machado Rocha e Robson Xavier Faria provided the space and the reagents; Robson Xavier Faria and André Luis Souza performed the experiments; Barbara Lima, Luis Armando CandidoTietbohl, Caio Pinho Fernandes, Raquel Rodrigues Amaral, Bettina Monika Ruppelt, Marcelo Guerra Santos collected the plants and prepared the extracts, Leandro Machado Rocha and Robson Xavier Faria wrote the manuscript. All authors have read and approved the final version of the manuscript.

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Correspondence to Robson Xavier Faria.

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Faria, R.X., Souza, A.L.A., Lima, B. et al. Plants of Brazilian restingas with tripanocide activity against Trypanosoma cruzi strains. J Bioenerg Biomembr 49, 473–483 (2017). https://doi.org/10.1007/s10863-017-9733-9

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  • DOI: https://doi.org/10.1007/s10863-017-9733-9

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