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TCP family genes control leaf development and its responses to hormonal stimuli in tea plant [Camellia sinensis (L.) O. Kuntze]

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Abstract

Tea plant [Camellia sinensis (L.) O. Kuntze] is a perennial evergreen woody crop that is cultivated worldwide. Tea leaf is an important resource for producing natural non-alcoholic beverages. TCP (TEOSINTE BRANCHED1/CYCLOIDEA/PROLIFERATING CELL FACTORS) is a transcription factor family that controls the cell growth and proliferation of leaf tissues. In this study, 17 homologous TCP family transcription factors were identified and characterized in the leaf transcriptome of C. sinensis. The structural features, phylogenetic tree, and interaction networks of C. sinensis TCP (CsTCP) proteins were analyzed. Prediction of miRNA target sites suggests that miR319 may be involved in the post-transcriptional regulation of the CsTCP15 transcript. The expression profiles of all identified CsTCP genes were investigated in five tea leaf developmental stages (i.e., 1st, 2nd, 3rd, 4th, and older leaves) and normal growth tea leaves subjected to five hormonal stimuli (i.e., ABA, GA3, IAA, MeJA, and SA). Several CsTCP genes presented functional redundancies in leaf development and response to hormones. This study establishes an extensive overview of the TCP family genes and provides insights into the molecular mechanism of leaf development and hormonal stimuli in C. sinensis.

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Abbreviations

ABA:

Abscisic acid

GA3 :

Gibberellic acid

IAA:

3-indoleacetic acid

MeJA:

Methyl jasmonate

qRT-PCR:

Quantitative real-time PCR

SA:

Salicylic acid

SMS:

Sequence Manipulation Suite

TBP:

TATA-box binding protein

TCP:

TEOSINTE BRANCHED 1/CYCLOIDEA/PROLIFERATING CELL FACTORS

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Acknowledgements

The work is supported by the National Natural Science Foundation of China (31570691).

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Authors and Affiliations

  1. Tea Science Research Institute, College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Zhi-Jun Wu, Wen-Li Wang & Jing Zhuang

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  1. Zhi-Jun Wu
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  2. Wen-Li Wang
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  3. Jing Zhuang
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Correspondence to Jing Zhuang.

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Wu, ZJ., Wang, WL. & Zhuang, J. TCP family genes control leaf development and its responses to hormonal stimuli in tea plant [Camellia sinensis (L.) O. Kuntze]. Plant Growth Regul 83, 43–53 (2017). https://doi.org/10.1007/s10725-017-0282-3

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