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Germplasm screening of Musa spp. for resistance to Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4)

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Abstract

Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4) has seriously threatened global banana production. Recently, identification of Foc TR4 in the Caribbean and Mozambique has aroused widespread concern because it may be directly related to food security for millions of people in Africa and Latin America. To identify a resistant or highly resistant germplasm, 129 accessions were evaluated for Foc TR4 resistance in greenhouses and 100 of them were also assessed in the field. In total, 10 accessions were highly resistant (HR) to Foc TR4; these include four from the AA group, two from the BB group, one of the East African highland bananas (EAHBs), two plantains, and one from wild relatives of banana. Of these, Pahang (AA), Calcutta 4 (AA), and Musa itinerans exhibited the highest degree of resistance with an index of disease (ID2) less than 10, and Foc TR4 could not infect any of them in the field. Furthermore, 31 accessions from AA, AB, AAB, AAAB, and ABB groups and wild relatives were identified as a resistant (R) germplasms. All tested EAHBs and plantains exhibited resistance or high resistance to Foc TR4 infection. Our results provide valuable genetic resources for banana breeding and for studying the mechanisms underlying Fusarium wilt resistance. Furthermore, evaluation of EAHBs and plantains provided a rational for local banana producers and researchers to grow EAHBS and plantains.

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Abbreviations

CARBAP:

Ctr Africain Rech Bananiers & Plantains

EAHBs:

East African Highland Bananas

Foc :

Fusarium oxysporum f. sp. cubense

Foc TR4:

Foc tropical race 4

Foc STR4:

Foc subtropical race 4

HR:

Highly resistant

HS:

Highly susceptible

I:

Immune

ID1:

Index of disease

ID2:

Incidence of disease

IITA:

International institute of tropical agriculture

ITC:

International Transit Center

MR:

Moderately resistant

PDA:

Potato dextrose agar

PDB:

Potato dextrose broth

R:

Resistant

S:

Susceptible

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Acknowledgements

We thank Dr. Zhongjian Chen (Argo-biological Gene Research Center, Guangdong Academy of Agricultural Sciences, Guangzhou, China) to revise and edit the final version of the manuscript.

Funding

This work was supported by the Natural Science Foundation of China No. 31501728 and 31,601,720, Global Crop Diversity Trust No. LOA 2010/50, International cooperation project No. 2014B050502007, and 948 Project from Ministry of Agriculture of China No. 2011-G16.

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Author notes

  1. Cunwu Zuo and Guiming Deng contributed equally to this work.

Authors and Affiliations

  1. Institute of Fruit Tree Research, Guangdong Academy of Agricultural Sciences (IFTR-GDAAS), Guangzhou, 510640, China

    Cunwu Zuo, Guiming Deng, Chunyu Li, Chunhua Hu, Ruibin Kuang, Qiaosong Yang, Tao Dong, Ou Sheng & Ganjun Yi

  2. College of Horticulture, Gansu Agricultural University, Lanzhou, 730070, China

    Cunwu Zuo

  3. Key Laboratory of South Subtropical Fruit Biology and Genetic Resource Utilization, Ministry of Agriculture, Guangzhou, 510640, China

    Guiming Deng, Chunyu Li, Chunhua Hu, Ruibin Kuang, Qiaosong Yang, Tao Dong, Ou Sheng & Ganjun Yi

  4. Collage of Life Science, South China Agricultural University, Guangzhou, 510640, China

    Bin Li

  5. Mid-Research and Education Center, University of Florida, Apopka, FL, 32703, USA

    Heqiang Huo

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  1. Cunwu Zuo
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Correspondence to Ou Sheng or Ganjun Yi.

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Fig. S1

Foc TR4 detection using specific primers. For each sample, different genomic DNA was used as a substrate for PCR amplification. C-: non-infected banana pseudostem; C+: Foc TR4 strain GD-13; 1 to 10: Foc strains isolated from infected banana pseudostem after inoculation. (GIF 603 kb)

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Zuo, C., Deng, G., Li, B. et al. Germplasm screening of Musa spp. for resistance to Fusarium oxysporum f. sp. cubense tropical race 4 (Foc TR4). Eur J Plant Pathol 151, 723–734 (2018). https://doi.org/10.1007/s10658-017-1406-3

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