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Phosphorylation of alfalfa mosaic virus movement protein in vivo

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Abstract

The 32-kDa movement protein, P3, of alfalfa mosaic virus (AMV) is essential for cell-to-cell spread of the virus in plants. P3 shares many properties with other virus movement proteins (MPs); however, it is not known if P3 is posttranslationally modified by phosphorylation, which is important for the function of other MPs. When expressed in Nicotiana tabacum, P3 accumulated primarily in the cell walls of older leaves or in the cytosol of younger leaves. When expressed in Pischia pastoris, P3 accumulated primarily in a soluble form. Metabolic labeling indicated that a portion of P3 was phosphorylated in both tobacco and yeast, suggesting that phosphorylation regulates the function of this protein as it does for other virus MPs.

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Acknowledgements

Karen J. Krahn (d. 2013) substantially contributed to this project by developing and analyzing the transgenic tobacco lines producing AMV P3 protein at Agrigenetics. We thank Nancy P. Jarvis for Southern and northern analyses of the transgenic lines and Prof. Lous van Vloting-Doting for providing the C-terminal peptide of AMV P3 for antibody production. BSK acknowledges support from Purdue Research Foundation, Purdue University.

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Author notes

  1. Edward L. Halk

    Present address: Bristol-Myers Squibb, Redwood City, CA, USA

  2. Donald J. Merlo

    Present address: , 11845 Durbin Drive, Carmel, IN, 46032, USA

  3. Steven E. Nelson

    Present address: , 4397 Gils Way, Cross Plains, WI, 53528, USA

Authors and Affiliations

  1. Department of Botany and Plant Pathology, Purdue University, West Lafayette, IN, 47907, USA

    Bong-Suk Kim & L. Sue Loesch-Fries

  2. Agrigenetics Advanced Science Company, Madison, WI, USA

    Edward L. Halk, Donald J. Merlo, Steven E. Nelson & L. Sue Loesch-Fries

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  1. Bong-Suk Kim
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  2. Edward L. Halk
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  5. L. Sue Loesch-Fries
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Correspondence to L. Sue Loesch-Fries.

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Kim, BS., Halk, E.L., Merlo, D.J. et al. Phosphorylation of alfalfa mosaic virus movement protein in vivo . Arch Virol 159, 1787–1791 (2014). https://doi.org/10.1007/s00705-013-1945-7

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