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Sequence analysis of the whole genome of a recombinant Marek’s disease virus strain, GX0101, with a reticuloendotheliosis virus LTR insert

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Abstract

Marek’s disease virus Chinese strain GX0101, isolated in 2001, is the first reported recombinant gallid herpesvirus type 2 (GaHV-2) field strain with one reticuloendotheliosis virus (REV) long terminal repeat (LTR) insert. We constructed an infectious bacterial artificial chromosome (BAC) clone of GX0101, which showed characteristics very similar to those of the parental virus in replication and pathogenicity. Using the GX0101 BAC clone, the complete genome of GX0101 was sequenced and analyzed. The length of the GX0101 genome is 178,101 bp, and it contains only one REV-LTR insert at a site 267 bp upstream of the sorf2 gene.

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References

  1. Lee LF, Wu P, Sui D, Ren D, Kamil J, Kung HJ, Witter RL (2000) The complete unique long sequence and the overall genomic organization of the GA strain of Marek’s disease virus. Proc Natl Acad Sci USA 97:6091–6096

    Article  PubMed  CAS  Google Scholar 

  2. Tulman ER, Afonso CL, Lu Z, Zsak L, Rock DL, Kutish GF (2000) The genome of a very virulent Marek’s disease virus. J Virol 74:7980–7988

    Article  PubMed  CAS  Google Scholar 

  3. Zhang Z, Cui Z (2005) Isolation of recombinant field strains of Marek’s disease virus integration with reticuloendotheliosis virus genome fragments. Sci China C Life Sci 48:81–88

    Article  PubMed  CAS  Google Scholar 

  4. Su S, Cui N, Cui Z, Zhao P, Li Y, Ding J, Dong X (2012) Complete genome sequence of a recombinant Marek’s disease virus field strain with one reticuloendotheliosis virus long terminal repeat insert. J Virol 86:13818–13819

    Article  PubMed  CAS  Google Scholar 

  5. Cui Z, Zhuang G, Xu X, Sun A, Su S (2010) Molecular and biological characterization of a Marek’s disease virus field strain with reticuloendotheliosis virus LTR insert. Virus Genes 40:236–243

    Article  PubMed  CAS  Google Scholar 

  6. Kost R, Jones D, Isfort R, Witter R, Kung HJ (1993) Retrovirus insertion into herpesvirus: characterization of an attenuated Marek’s disease virus harboring a solo LTR. Virology 192:161–169

    Article  PubMed  CAS  Google Scholar 

  7. Isfort RJ, Jones D, Kost R, Witter R, Kung HJ (1992) Retrovirus insertion into herpesvirus in vitro and in vivo. Proc Natl Acad Sci USA 89:991–995

    Article  PubMed  CAS  Google Scholar 

  8. Jones D, Brunovskis P, Witter R, Kung HJ (1996) Retroviral insertional activation in a herpesvirus: transcriptional activation of US genes by an integrated long terminal repeat in a Marek’s disease virus clone. J Virol 70:2460–2467

    PubMed  CAS  Google Scholar 

  9. Sun A, Xu X, Petherbridge L, Zhao Y, Nair V, Cui Z (2010) Functional evaluation of the role of reticuloendotheliosis virus long terminal repeat (LTR) integrated into the genome of a field strain of Marek’s disease virus. Virology 397:270–276

    Article  PubMed  CAS  Google Scholar 

  10. Sun A, Petherbridge L, Zhao Y, Li Y, Nair V, Cui Z (2009) A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity. Chinese Science Bulletin 54:2641–2647

    Article  CAS  Google Scholar 

  11. Zhang F, Liu C, Zhang Y, Li Z, Liu A, Yan F, Cong F, Cheng Y (2012) Comparative full-length sequence analysis of Marek’s disease virus vaccine strain 814. Arch Virol 157:177–183

    Article  PubMed  CAS  Google Scholar 

  12. Spatz SJ, Smith LP, Baigent SJ, Petherbridge L, Nair V (2011) Genotypic characterization of two bacterial artificial chromosome clones derived from a single DNA source of the very virulent gallid herpesvirus-2 strain C12/130. J Gen Virol 92:1500–1507

    Article  PubMed  CAS  Google Scholar 

  13. Spatz SJ, Petherbridge L, Zhao Y, Nair V (2007) Comparative full-length sequence analysis of oncogenic and vaccine (Rispens) strains of Marek’s disease virus. J Gen Virol 88:1080–1096

    Article  PubMed  CAS  Google Scholar 

  14. Spatz SJ, Zhao Y, Petherbridge L, Smith LP, Baigent SJ, Nair V (2007) Comparative sequence analysis of a highly oncogenic but horizontal spread-defective clone of Marek’s disease virus. Virus Genes 35:753–766

    Article  PubMed  CAS  Google Scholar 

  15. Parcells MS, Anderson AS, Morgan TW (1995) Retention of oncogenicity by a Marek’s disease virus mutant lacking six unique short region genes. J Virol 69:7888–7898

    PubMed  CAS  Google Scholar 

  16. Kim T, Mays J, Fadly A, Silva RF (2011) Artificially inserting a reticuloendotheliosis virus long terminal repeat into a bacterial artificial chromosome clone of Marek’s disease virus (MDV) alters expression of nearby MDV genes. Virus Genes 42:3693–3676

    Google Scholar 

  17. Mays JK, Silva RF, Kim T, Fadly A (2012) Insertion of reticuloendotheliosis virus long terminal repeat into a bacterial artificial chromosome clone of a very virulent Marek’s disease virus alters its pathogenicity. Avian Pathol 41:259–265

    Article  PubMed  CAS  Google Scholar 

  18. Barrow A, Venugopal K (1999) Molecular characteristics of very virulent European MDV isolates. Acta Virol 43:90–93

    PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by grants from Guangdong Joint Funds from the National Natural Science Foundation of China (U1131005).

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Authors and Affiliations

  1. College of Veterinary Medicine, Shandong Agricultural University, Tai’an, Shandong, 271018, China

    Shuai Su, Ning Cui, Zimeng Chen, Peng Zhao & Zhizhong Cui

  2. Department of Veterinary Pathobiology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, 77843, United States

    Aijun Sun

  3. Biotechnology research institute, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Yanpeng Li

  4. China Institute of Veterinary Drug Control, Beijing, 100081, China

    Jiabo Ding

Authors
  1. Shuai Su
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  2. Ning Cui
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  3. Aijun Sun
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  4. Yanpeng Li
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  5. Jiabo Ding
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  6. Zimeng Chen
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  7. Peng Zhao
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  8. Zhizhong Cui
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Corresponding author

Correspondence to Zhizhong Cui.

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S. Su and N. Cui contributed equally to this work.

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Su, S., Cui, N., Sun, A. et al. Sequence analysis of the whole genome of a recombinant Marek’s disease virus strain, GX0101, with a reticuloendotheliosis virus LTR insert. Arch Virol 158, 2007–2014 (2013). https://doi.org/10.1007/s00705-013-1671-1

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  • DOI: https://doi.org/10.1007/s00705-013-1671-1

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